Ribozyme-mediated compensatory induction of menin-oncosuppressor function in primary fibroblasts from MEN1 patients

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Origini e sviluppo di alcune infrastrutture di rilievo nell\u2019area a nord-ovest di Firenze dall\u2019epoca granducale al secondo dopoguerra

Nella periferia fiorentina sono ancora presenti anche in forma di archeologia industriale una serie di infrastrutture realizzate fra Ottocento e Novecent

Premessa all'Inventario della corrispondenza di Gaetano Salvemini

Prersentazione della corrispondenza di Gaetano Salvemini, presente nell'Archivio dello storico e uomo politico e conservata a Firenze presso l'Istituto per la storia della resistenza in Toscana e, in microfilm, presso gli Archivi dell'Unione Europe

Inhibition of In Vitro Growth and Arrest in the G0/G1 Phase of HCT8 Line Human Colon Cancer Cells by Kaempferide Triglycoside from Dianthus caryophyllus

The effects of phytoestrogens have been studied in the hypothalamic-pituitary-gonadal axis and in various non-gonadal targets. Epidemiologic and experimental evidence indicates a protective effect of phytoestrogens also in colorectal cancer. The mechanism through which estrogenic molecules control colorectal cancer tumorigenesis could possibly involve estrogen receptor beta, the predominantly expressed estrogen receptor subtype in colon mucosa. To validate this hypothesis, we therefore used an engineered human colon cancer cell line induced to overexpress estrogen receptor beta, beside its native cell line, expressing very low levels of ER beta and not expressing ER alpha; as a phytoestrogenic molecule, we used kaempferide triglycoside, a glycosylated flavonol from a Dianthus caryophyllus cultivar. The inhibitory properties of this molecule toward vegetal cell growth have been previously demonstrated: however, no data on its activity on animal cell or information about the mechanism of this activity are available. Kaempferide triglycoside proved to inhibit the proliferation of native and estrogen receptor beta overexpressing colon cancer cells through a mechanism not mediated by ligand binding dependent estrogen receptor activation. It affected HCT8 cell cycle progression by increasing the G(0)/G(1) cell fraction and in estrogen receptor beta overexpressing cells increased two antioxidant enzymes. Interestingly, the biological effects of this kaempferide triglycoside were strengthened by the presence of high levels of estrogen receptor beta. Pleiotropic molecular effects of phytoestrogens may explain their protective activity against colorectal cancer and may represent an interesting area for future investigation with potential clinical applications. Copyright (C) 2010 John Wiley & Sons, Ltd