Personal, Social and Emotional Development

Desarrolla el cumplimiento de los requisitos exigidos por la Autoridad Nacional (AUNA) para que junto a otros 9 capítulos justifiquen la modificación del núcleo debido al cambio del tipo de elementos combustibles de U3O8 por elementos combustibles del tipo U3Si2. Estas modificaciones cambian las condiciones del diseño original y afecta solo a algunos parámetros de seguridad alrededor del núcleo, sin embargo los informes denominados IAS (Informe de Análisis de Seguridad) que acompañan a todo diseño de un reactor nuclear deberán ser revaluados y actualizados como medida para incrementar la confiabilidad del uso de la instalación. Este trabajo concluye con resultados que cumplen tanto con los requerimientos de AUNA y con los requerimientos actuales del Organismo Internacional de Energía Atómica, la cual lo hace aceptable para los trámites de modificación del núcleo considerando el cambio de elementos combustibles de U3O8 a U3Si2

Biochemical and biophysical characterization of four EphB kinase domains reveals contrasting thermodynamic, kinetic and inhibition profiles

The Eph (erythropoietin-producing hepatocellular carcinoma) B receptors are important in a variety of cellular processes through their roles in cell-to-cell contact and signalling; their up-regulation and down-regulation has been shown to have implications in a variety of cancers. A greater understanding of the similarities and differences within this small, highly conserved family of tyrosine kinases will be essential to the identification of effective therapeutic opportunities for disease intervention. In this study, we have developed a route to production of multi-milligram quantities of highly purified, homogeneous, recombinant protein for the kinase domain of these human receptors in Escherichia coli. Analyses of these isolated catalytic fragments have revealed stark contrasts in their amenability to recombinant expression and their physical properties: e.g., a >16°C variance in thermal stability, a 3-fold difference in catalytic activity and disparities in their inhibitor binding profiles. We find EphB3 to be an outlier in terms of both its intrinsic stability, and more importantly its ligand-binding properties. Our findings have led us to speculate about both their biological significance and potential routes for generating EphB isozyme-selective small-molecule inhibitors. Our comprehensive methodologies provide a template for similar in-depth studies of other kinase superfamily members

Challenging the problem of 'fit': advancing the regenerative medicine industries in the United States, Britain and Japan

This article follows the evolution of biopharmaceutical firms as they bore great uncertainty and risks in their endeavours to commercialise new therapies – through the regenerative medicine industry in the United States, Britain and Japan. Despite its beginnings in the 1970s, regenerative medicines have yet to become a widely accepted form of medicine. A large part of the problem lay with the lack of ‘fit’ with the broader health context. This article illustrates how the trajectory of the sector was shaped, not only by the nature of the technology, but also by the complex contexts in which firms were embedded