The influence of ethanol as a solvent on the gibberellic acid-induced germination of Brachyscome and Allittia (Asteraceae) seeds

Published online 16 February 2018Gibberellic acid (GA₃) is routinely used as a germination stimulant for seeds. However, the methods used to dissolve GA₃ powder – particularly if using organic solvents – have the potential to affect germination outcomes. In this study we examined the influence of the solvent ethanol, used to dissolve GA₃, on the seed germination of 14 species of Brachyscome and two species of Allittia. These species are important Australian native composites with potential for use in habitat restoration. Seeds of 11 of these species were found to be particularly responsive to GA₃. However, the use of a low concentration of ethanol (0.5%) to dissolve GA₃ affected subsequent germination outcomes, with four species responding positively and eight negatively to this solvent. The pure effect of GA₃ was therefore masked by the presence of small concentrations of ethanol, whose effects varied between the species examined. Because of these potentially confounding effects of ethanol, we recommend the use of pure water for dissolution of GA₃ when testing seed germination responses in these genera.Rina Aleman, Manfred Jusaitis, Joan Gibbs, Phil Ainsley and Fleur Tive

Influence of seed dimorphism and provenance on seed morphology, dispersal, germination and seedling growth of Brachyscome ciliaris (Asteraceae)

Brachyscome ciliaris is a floriferous Australian native daisy, with potential for use as a horticultural species. The species is hardy and seeds are relatively easy to germinate, but it is unique within the Brachyscome genus in that seeds are distinctly dimorphic. Within a fruiting capitulum, ray seeds are smooth and narrow with a minute pappus, whereas disc seeds have broad flat wings with curled hairs and a longer pappus than that of ray seeds. Both seed morphs, collected from five populations of the species, were tested to determine differences in their morphology, germination speed and percentage, seedling growth and wind-dispersal characteristics. Ray seeds were generally lighter and smaller than disc seeds and their length varied significantly with provenance. Dormancy levels of the two seed morphs and growth of ray- and disc-derived seedlings did not differ significantly, but differences were significant among the five populations tested. Seeds germinated readily, and germination was optimal under winter or summer conditions and lower in spring or autumn. Seed production by plants raised from ray or disc seeds was identical, but Noora-sourced plants yielded more seed than did plants sourced from the other provenances tested. Seed size, germination and plant growth of B. ciliaris varied significantly among populations. Winged disc seeds were dispersed slightly further by wind than were wingless ray seeds. We concluded that dormancy, germination and seed-yield characteristics of B. ciliaris were all influenced more by seed provenance than by seed morph (ray or disc).Rina Aleman, Manfred Jusaitis, Joan Gibbs, Phillip Ainsley, Fleur Tiver and Sophie Peti

The inspection paradox: an important consideration in the evaluation of rotor lifetimes in cardiac fibrillation

Background and Objective: Renewal theory is a statistical approach to model the formation and destruction of phase singularities (PS), which occur at the pivots of spiral waves. A common issue arising during observation of renewal processes is an inspection paradox, due to oversampling of longer events. The objective of this study was to characterise the effect of a potential inspection paradox on the perception of PS lifetimes in cardiac fibrillation. Methods: A multisystem, multi-modality study was performed, examining computational simulations (Aliev-Panfilov (APV) model, Courtmanche-Nattel model), experimentally acquired optical mapping Atrial and Ventricular Fibrillation (AF/VF) data, and clinically acquired human AF and VF. Distributions of all PS lifetimes across full epochs of AF, VF, or computational simulations, were compared with distributions formed from lifetimes of PS existing at 10,000 simulated commencement timepoints. Results: In all systems, an inspection paradox led towards oversampling of PS with longer lifetimes. In APV computational simulations there was a mean PS lifetime shift of +84.9% (95% CI, ± 0.3%) (p < 0.001 for observed vs overall), in Courtmanche-Nattel simulations of AF +692.9% (95% CI, ±57.7%) (p < 0.001), in optically mapped rat AF +374.6% (95% CI, ± 88.5%) (p = 0.052), in human AF mapped with basket catheters +129.2% (95% CI, ±4.1%) (p < 0.05), human AF-HD grid catheters 150.8% (95% CI, ± 9.0%) (p < 0.001), in optically mapped rat VF +171.3% (95% CI, ±15.6%) (p < 0.001), in human epicardial VF 153.5% (95% CI, ±15.7%) (p < 0.001). Conclusion: Visual inspection of phase movies has the potential to systematically oversample longer lasting PS, due to an inspection paradox. An inspection paradox is minimised by consideration of the overall distribution of PS lifetimes

Metastatic epidural spinal cord compression: current concepts and treatment

Metastatic epidural spinal cord compression (MESCC) is a medical emergency complicating the course of 5–10% of patients with cancer [1]. When diagnosis and treatment is early with the patient ambulatory prognosis for continued ambulation is good [2]. If the patient is nonambulatory or paraplegic, prognosis for meaningful recovery of motor and bladder function is markedly decreased. In the last decade, significant advances in the understanding, management and treatment of metastatic epidural spinal cord compression have occurred.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45378/1/11060_2005_Article_BF01051052.pd

Parameter esimation for stage-structured projection models using real data

Not availabl

Low density of prickly acacia under sheep grazing in Queensland

Populations of an introduced woody weed, prickly acacia (Acacia nilotica (L.) Delile ssp. indica (Benth.) Brenan syn. Acacia arabica (Lam.) Willd. ssp. indica Benth.), were surveyed at 4 sites in central Queensland. There is a significantly lower frequency of plants of 3 m in height within populations which have been grazed by sheep, indicating that browsing by sheep reduces regeneration. There were higher losses of seedlings at a sheep-grazed site than at cattle-grazed sites. These results support previous assertions that prickly acacia is regenerating more successfully on cattle properties, because cattle both disperse seeds and are less effective herbivores. In regions of low annual rainfall, prickly acacia is capable of forming dense stands (up to 2,700 shrubs ha(-1)) in lowland landscape types. Stands are less dense in upland landscapes (maximum of 718 shrubs ha(-1)). Of most concern is that in regions of high annual rainfall prickly acacia can form extremely dense thickets across most landscape types (up to 3,400 shrubs ha(-1)). We suggest that prickly acacia is most likely to become a management problem on cattle properties, and an extreme problem in high annual rainfall areas. The inclusion of sheep in livestock rotations may be an effective control measure in the Mitchell Grasslands, but this may not always be possible. A high priority is to prevent prickly acacia from expanding its range into equivalent high rainfall areas within Queensland, and also in the Northern Territory, northern New South Wales, and Western Australia. This could be achieved by quarantining livestock which have come from infested properties until seeds have passed through the digestive tract, after about 6 days. Management strategies at the property level should aim to prevent further spread of prickly acacia by controlling cattle movements between paddocks during periods when cattle are ingesting pods and seeds.The Journal of Range Management archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform August 202

THE COMPOSITION AND DISTRIBUTION OF THE VEGETATION OF NORTHWEST EYRE PENINSULA AUSTRALIA

Volume: 113Start Page: 47End Page: 6

Germination requirements of Brachyscome species in South Australia

The genus Brachyscome, in the daisy family, Asteraceae, has approximately 75 Australian species, many of conservation significance and at risk of extinction in the wild (Salkin et al. 1995). Brachyscome plants are annual or perennial herbs or subshrubs, with characteristic, solitary flower heads of a single row of ray florets that are white, blue, pink or mauve. Most notable of the South Australian Brachyscomes are the endangered species: B. decipiens, B. diversifolia, and B. muelleri (National Parks and Wildlife Act 1972).Rina Aleman, Phil Ainsley, Joan Gibbs, Fleur Tiver and Manfred Jusaiti