Magnetic resonance detects metabolic changes associated with chemotherapy-induced apoptosis

Apoptosis was induced by treating L1210 leukaemia cells with mechlorethamine, and SW620 colorectal cells with doxorubicin. The onset and progression of apoptosis were monitored by assessing caspase activation, mitochondrial transmembrane potential, phosphatidylserine externalization, DNA fragmentation and cell morphology. In parallel, 31P magnetic resonance (MR) spectra of cell extracts were recorded. In L1210 cells, caspase activation was detected at 4 h. By 3 h, the MR spectra showed a steady decrease in NTP and NAD, and a significant build-up of fructose 1,6-bisphosphate (F-1,6-P) dihydroxyacetonephosphate and glycerol-3-phosphate, indicating modulation of glycolysis. Treatment with iodoacetate also induced a build-up of F-1,6-P, while preincubation with two poly(ADP-ribose) polymerase inhibitors, 3-aminobenzamide and nicotinamide, prevented the drop in NAD and the build-up of glycolytic intermediates. This suggested that our results were due to inhibition of glyceraldehyde-3-phosphate dehydrogenase, possibly as a consequence of NAD depletion following poly(ADP-ribose) polymerase activation. Doxorubicin treatment of the adherent SW620 cells caused cells committed to apoptosis to detach. F-1,6-P was observed in detached cells, but not in treated cells that remained attached. This indicated that our observations were not cell line- or treatment-specific, but were correlated with the appearance of apoptotic cells following drug treatment. The 31P MR spectrum of tumours responding to chemotherapy could be modulated by similar effects

A 5 ̊C Arctic in a 2 ̊C World

The Columbia Climate Center, in partnership with World Wildlife Fund, Woods Hole Research Center, and Arctic 21, held a workshop titled A 5 C Arctic in a 2 C World on July 20 and 21, 2016. The workshop was co-sponsored by the International Arctic Research Center (University of Alaska Fairbanks), the Arctic Institute of North America (Canada), the MEOPAR Network (Marine Environmental Observation, Prediction, and Response), and the Future Ocean Excellence Cluster. The goal of the workshop was to advance thinking on the science and policy implications of the temperature change in the context of the 1.5 to 2 C warming expected for the globe, as dis- cussed during the 21st session of the Conference of the Parties of the United Nations Framework Convention on Climate Change at Paris in 2015. For the Arctic, such an increase means an antic- ipated increase of roughly 3.5 to 5 C. An international group of 41 experts shared perspectives on the regional and global impacts of an up to +5 C Arctic, examined the feasibility of actively lowering Arctic temperatures, and considered realistic timescales associated with such interventions. The group also discussed the science and the political and governance actions required for alternative Arctic futures

The Anti-Racist Media Manifesto

The rise of the far-right, the impacts of Covid-19, and the mediated evidence of racist police violence have challenged the dominant complacency in liberal democracies that racism was a thing of the past. We are now witnessing the renewed anti-racist commitment of social movements and the rising authoritarianism that seeks to suppress it. This ongoing tension provides important opportunities for reflection and intervention in how institutions deal with the problem of racism, including media institutions. Rather than making media “less racist”, how can media systems, policies and practices be transformed in ways that actively challenge the production of racism? What should a truly anti-racist media look like? Anamik Saha, Francesca Sobande and Gavan Titley address these important and timely questions to outline the essential steps for working towards an anti-racist media future. Revealing how the media are implicated in racism, the authors consider how systems, policies and practices can be transformed to confront and prevent it. Focusing on the problems of impartiality, the limits of diversity and representation, and the contradictions of digital culture, this manifesto embraces anti-racism’s collectivist roots. Ultimately, the book illuminates key strategies and suggestions to move us closer to an anti-racist media future for everyone. The Anti-Racist Media Manifesto is a must-read for students, scholars, media workers and activists in the fields of journalism, media, policy, and sociology, as well as general readers

Using ensembles to analyse predictability links in the tropical cyclone flood forecast chain

Fluvial flooding is a major cause of death and damages from tropical cyclones (TCs), so it is important to understand the predictability of river flooding in TC cases, and the potential of global ensemble flood forecast systems to inform warning and preparedness activities. This paper demonstrates a methodology using ensemble forecasts to follow predictability and uncertainty through the forecast chain in the Global Flood Awareness System (GloFAS), to explore the connections between the skill of the TC track, intensity, precipitation and river discharge forecasts. Using the case of Hurricane Iota, which brought severe flooding to Central America in November 2020, we assess the performance of each ensemble member at each stage of the forecast, along with the overall spread and change between forecast runs, and analyse the connections between each forecast component. Strong relationships are found between track, precipitation and river discharge skill. Changes in TC intensity skill only result in significant improvements in discharge skill in river catchments close to the landfall location that are impacted by the heavy rains around the eye wall. The rainfall from the wider storm circulation is crucial to flood impacts in most of the affected river basins, with a stronger relationship with the post-landfall track error rather than the precise landfall location. We recommend the wider application of this technique in TC cases, to investigate how this cascade of predictability varies with different forecast and geographical contexts, to help inform flood early warning in TCs

Key factors influencing the severity of fluvial flood hazard from tropical cyclones

Knowledge of the key drivers of the severity of river flooding from tropical cyclones (TCs) is vital for emergency preparedness and disaster risk reduction activities. This global study examines landfalling TCs in the decade from 2010 to 2019, to identify those characteristics that influence whether a storm has an increased flood hazard. The highest positive correlations are found between flood severity and the total precipitation associated with the TC. Significant negative correlations are found between flood severity and the translation speed of the TC, indicating that slower moving storms, that rain over an area for longer, tend to have higher flood severity. Larger and more intense TCs increase the likelihood of having a larger area affected by severe flooding but not its duration or magnitude, and it is found that the fluvial flood hazard can be severe in all intensity categories of TC, including those of tropical storm strength. Catchment characteristics such as antecedent soil moisture and slope also play a role in modulating flood severity, and severe flooding is more likely in cases where multiple drivers are present. The improved knowledge of the key drivers of fluvial flooding in TCs can help to inform research priorities to help with flood early warning, such as increasing the focus on translation speed in model evaluation and impact-based forecasting

Is bovine dentine an appropriate substitute in abrasion studies

The study aimed to compare the wear behaviour of human and bovine dentine due to toothbrushing with different relative dentin abrasivity (RDA) toothpastes. Forty human and 40 bovine dentine samples were prepared from bovine lower incisors or human premolars roots, and baseline surface profiles were recorded. The samples were distributed to four groups (each group n = 10 human and 10 bovine samples) and brushed with fluoridated experimental toothpastes with different RDAs (group A: RDA 10, B: RDA 20, C: RDA 50, and D: RDA 100). Toothbrushing was performed in an automatic brushing machine with a brushing frequency of 60 strokes per minute and a brushing force of 2.5 N. After 2, 5, 10, and 25 min of toothbrushing, new surface profiles were recorded, and the dentine wear was calculated with a customised computer programme. The dentine wear of human and bovine dentine within the four groups was compared with unpaired t tests. No statistically significant difference was recorded for the dentine wear of human and bovine samples within the different groups