Transgenic Plants That Express the Phytoplasma Effector SAP11 Show Altered Phosphate Starvation and Defense Responses

Phytoplasmas have the smallest genome among bacteria and lack many essential genes required for biosynthetic and metabolic functions, making them unculturable, phloem-limited plant pathogens. In this study, we observed that transgenic Arabidopsis (Arabidopsis thaliana) expressing the secreted Aster Yellows phytoplasma strain Witches' Broom protein11 shows an altered root architecture, similarly to the disease symptoms of phytoplasma-infected plants, by forming hairy roots. This morphological change is paralleled by an accumulation of cellular phosphate (Pi) and an increase in the expression levels of Pi starvation-induced genes and microRNAs. In addition to the Pi starvation responses, we found that secreted Aster Yellows phytoplasma strain Witches' Broom protein11 suppresses salicylic acid-mediated defense responses and enhances the growth of a bacterial pathogen. These results contribute to an improved understanding of the role of phytoplasma effector SAP11 and provide new insights for understanding the molecular basis of plant-pathogen interactions

Phytoplasma SAP11 alters 3-isobutyl-2-methoxypyrazine biosynthesis in Nicotiana benthamiana by suppressing NbOMT

Phytoplasma effector SAP11 modulates plant volatile organic compound emissions by suppressing the expression of NbOMT1, which encodes an O-methyltransferase required for the biosynthesis of 3-isobutyl-2-methoxypyrazine.Phytoplasmas are bacterial phytopathogens that release virulence effectors into sieve cells and act systemically to affect the physiological and morphological state of host plants to promote successful pathogenesis. We show here that transgenic Nicotiana benthamiana lines expressing the secreted effector SAP11 from Candidatus Phytoplasma mali exhibit an altered aroma phenotype. This phenomenon is correlated with defects in the development of glandular trichomes and the biosynthesis of 3-isobutyl-2-methoxypyrazine (IBMP). IBMP is a volatile organic compound (VOC) synthesized by an O-methyltransferase, via a methylation step, from a non-volatile precursor, 3-isobutyl-2-hydroxypyrazine (IBHP). Based on comparative and functional genomics analyses, NbOMT1, which encodes an O-methyltransferase, was found to be highly suppressed in SAP11-transgenic plants. We further silenced NbOMT1 through virus-induced gene silencing and demonstrated that this enzyme influenced the accumulation of IBMP in N. benthamiana. In vitro biochemical analyses also showed that NbOMT1 can catalyse IBHP O-methylation in the presence of S-adenosyl-L-methionine. Our study suggests that the phytoplasma effector SAP11 has the ability to modulate host VOC emissions. In addition, we also demonstrated that SAP11 destabilized TCP transcription factors and suppressed jasmonic acid responses in N. benthamiana. These findings provide valuable insights into understanding how phytoplasma effectors influence plant volatiles

Recapitalization of Amphibious Operation and Lift

Includes supplementary material.CAPSTONE PROJECT REPORTSystem Engineering and Analysis Cohort 18A and Temasek Defense Systems InstituteThe aging Whidbey Island and Harpers Ferry class ships, LSD-41 and 49 respectively, comprise just over one third of the amphibious navy. However, a solution to the capability gap created by the loss of these ships is needed to maintain the effectiveness of the amphibious fleet across a broad spectrum of mission areas. This research effort considers future ship designs and fleet architectures to meet the capability gaps left by the decommissioning of the LSD-41 and 49 class ships. With respect to lift capacity, performance capability, cost and a risk assessment, the analysis showed the LPD-17 or a LSD(X) approximately 30% larger than the existing classes to be acceptable replacement classes. This analysis also supports further research to determine the most robust fleet architecture apart from the current eleven LHA or LHD, eleven LPD and eleven LSD paradigm.Approved for public release; distribution is unlimited

Pharmacogenomic Prediction of Bleomycin-Induced Pneumonitis in South East Asian Hodgkin Lymphoma Patients

10.1182/blood-2018-99-11395260th Annual Meeting of the American-Society-of-Hematology (ASH)132Supplement