Conversion of the Kunitz-type module of collagen VI into a highly active trypsin inhibitor by site-directed mutagenesis.

The recombinant Kunitz protease inhibitor module (domain C5) of human collagen α3(VI) chain was previously shown to lack inhibitory activity for proteases with trypsin-like specificity and some other proteases. We have now prepared mutants in the binding loop region including the P1′ site (D2889 → A), the P2′ site (F2890 → R) and the P3 site (T2886 → P) and in a more remote region (W2907 → V) either as individual substitutions or combinations of them. These mutants were analyzed for their kinetics of binding to trypsin by surface plasmon resonance and for their capacity to inhibit various proteases. Single substitutions (D → A, T → P, W → V) showed an effect only for D → A which bound to trypsin with Kd= 0.25 μM. A 25–100-fold increase in affinity was observed for the double mutants T → P/D → A and F → R/D → A and approached the affinity of aprotinin (Kd≈0.01 nM) in two different triple mutants. These affinities correlated well with the inhibitory capacities of the mutants for trypsin in the cleavage of a large protein and a small peptide substrate. A similar but not completely identical improvement in inhibitory capacity was also observed for leucocyte elastase but not for thrombin. These data could be interpreted in terms of steric interferences or lack of hydrogen bonding of a few critical residues based on three-dimensional structures available for the C5 domain

Theory of Incompressible States in a Narrow Channel

We report on the properties of a system of interacting electrons in a narrow channel in the quantum Hall effect regime. It is shown that an increase in the strength of the Coulomb interaction causes abrupt changes in the width of the charge-density profile of translationally invariant states. We derive a phase diagram which includes many of the stable odd-denominator states as well as a novel fractional quantum Hall state at lowest half-filled Landau level. The collective mode evaluated at the half-filled case is strikingly similar to that for an odd-denominator fractional quantum Hall state.Comment: 4 pages, REVTEX, and 4 .ps file

Large transconductance oscillations in a single-well vertical Aharonov-Bohm interferometer

Aharonov-Bohm (AB) interference is reported for the first time in the conductance of a vertical nanostructure based on a single GaAs/AlGaAs quantum well (QW). The two lowest subbands of the well are spatially separated by the Hartree barrier originating from electronic repulsion in the modulation-doped QW and provide AB two-path geometry. Split-gates control the in-plane electronic momentum dispersion. In our system, we have clearly demonstrated AB interference in both electrostatic and magnetic modes. In the latter case the magnetic field was applied parallel to the QW plane, and perpendicular to the 0.02 um^2 AB loop. In the electrostatic mode of operation the single-QW scheme adopted led to large transconductance oscillations with relative amplitudes exceeding 30 %. The relevance of the present design strategy for the implementation of coherent nanoelectronic devices is underlined.Comment: Accepted for publication on Physical Review B Rapid Communication

Finite Size Analysis of Luttinger Liquids with a source of 2k_f Scattering

Numerical analysis of the spectrum of large finite size Luttinger liquids (g<1) in the presence of a single source of 2k_f scattering has been made possible thanks to an effective integration of high degrees of freedom. Presence of irrelevant operators and their manifestation in transport are issues treated independently. We confirm the existence of two irrelevant operators: particle hopping and charge oscillations, with regions of dominance separated by g=1/2. Temperature dependence of conductance is shown to be dominated by hopping alone. Frequency dependence is affected by both irrelevant operators.Comment: 4 pages, LaTex (RevTex), 3 PostScript figures appende

Renormalization group study of the conductances of interacting quantum wire systems with different geometries

We examine the effect of interactions between the electrons on the conductances of some systems of quantum wires with different geometries. The systems include a wire with a stub in the middle, a wire containing a ring which can enclose a magnetic flux, and a system of four wires which are connected in the middle through a fifth wire. Each of the wires is taken to be a weakly interacting Tomonaga-Luttinger liquid, and scattering matrices are introduced at all the junctions. Using a renormalization group method developed recently for studying the flow of scattering matrices for interacting systems in one dimension, we compute the conductances of these systems as functions of the temperature and the wire lengths. We present results for all three regimes of interest, namely, high, intermediate and low temperature. These correspond respectively to the thermal coherence length being smaller than, comparable to and larger than the smallest wire length in the different systems, i.e., the length of the stub or each arm of the ring or the fifth wire. The renormalization group procedure and the formulae used to compute the conductances are different in the three regimes. We present a phenomenologically motivated formalism for studying the conductances in the intermediate regime where there is only partial coherence. At low temperatures, we study the line shapes of the conductances versus the electron energy near some of the resonances; the widths of the resonances go to zero with decreasing temperature. Our results show that the conductances of various systems of experimental interest depend on the temperature and lengths in a non-trivial way when interactions are taken into account.Comment: Revtex, 17 pages including 15 figure

Characterization of one-dimensional quantum channels in InAs/AlSb

We report the magnetoresistance characteristics of one-dimensional electrons confined in a single InAs quantum well sandwiched between AlSb barriers. As a result of a novel nanofabrication scheme that utilizes a 3nm-shallow wet chemical etching to define the electrostatic lateral confinement, the system is found to possess three important properties: specular boundary scattering, a strong lateral confinement potential, and a conducting channel width that is approximately the lithography width. Ballistic transport phenomena, including the quenching of the Hall resistance, the last Hall plateau, and a strong negative bend resistance, are observed at 4K in cross junctions with sharp corners. In a ring geometry, we have observed Aharonov-Bohm interference that exhibits characteristics different from those of the GaAs counterpart due to the ballistic nature of electron transport and the narrowness of the conducting channel width.Comment: pdf-file, 8 figures, to be published in Phys. Rev.

Observation of Quantum Asymmetry in an Aharonov-Bohm Ring

We have investigated the Aharonov-Bohm effect in a one-dimensional GaAs/GaAlAs ring at low magnetic fields. The oscillatory magnetoconductance of these systems are for the first time systematically studied as a function of density. We observe phase-shifts of $\pi$ in the magnetoconductance oscillations, and halving of the fundamental $h/e$ period, as the density is varied. Theoretically we find agreement with the experiment, by introducing an asymmetry between the two arms of the ring.Comment: 4 pages RevTex including 3 figures, submitted to Phys. Rev.

Electron transport in coupled chains of interacting fermions with impurities

We study the low-temperature transport of a doped two-chain ladder system of interacting fermions in the presence of a barrier or of a low concentration of impurities. Above a certain value of the interaction, the conductance is suppressed, like for a single chain, despite the presence of dominant superconducting correlations. There is, however, a region of repulsive interaction where perfect transmission across the barrier occurs unlike the single-chain case. We provide a possible explanation for the temperature maximum of the resistivity in the normal state of \srca.Comment: 4 pages, 2 figures, to be published in Phys. Rev. Let

Diffraction of complex molecules by structures made of light

We demonstrate that structures made of light can be used to coherently control the motion of complex molecules. In particular, we show diffraction of the fullerenes C60 and C70 at a thin grating based on a standing light wave. We prove experimentally that the principles of this effect, well known from atom optics, can be successfully extended to massive and large molecules which are internally in a thermodynamic mixed state and which do not exhibit narrow optical resonances. Our results will be important for the observation of quantum interference with even larger and more complex objects.Comment: 4 pages, 3 figure