Management of threats and errors in normal operations of assistant controllers : a thesis presented in partial fulfilment of the requirements for the degree of Master in Aviation at Massey University

"To err is indeed human, so to err is normal" Human errors are usually pronounced in accident or incident reports. Seldom does one pay enough attention to these errors during daily normal operations as these either go unnoticed or unreported for whatsoever the reasons may be. Therefore, the causes of these errors and also the system threats prevalent in the daily operations may not be fully contained. On the other hand, problematic situations that are successfully tackled by human skills are quite often treated as less important than they really are. The job of an assistant controller (AC) is one of the important domains in air traffic management (ATM). The AC work together with air traffic controllers as team members and they do have direct and indirect contributions to the safe, orderly and efficient flow of air traffic. In this study, the threats, errors and potential undesired states occurring with AC during normal operations will be recorded by a methodology, which is new to Hong Kong Air Traffic Control (ATC). This methodology, called Normal Operations Safety Observation (NOSO), is built on the Threat and Error Management (TEM) framework. The results will generate a broad outline on what sorts of threats, errors and undesired states an AC can be facing during normal operations. The relative frequencies of occurrence of these conditions will be presented separately in tables and figures. The AC's potential vulnerabilities and capabilities to cope with these threats, errors and undesired states will be discussed together with a suggested ranking. It is envisaged that an analysis of the data collected will aid the development and evaluation of safety defence measures in ATM and further support the applicability of this data collection methodology in other ATM operations and subsequent researches. KEYWORDS:- Normal Operations Safety Observation, Threat and Error Management, Safety Management, Air Traffic Control

REAL TIME RAMAN QUANTIFICATION WITHOUT A SPECTROMETER

Department of Biomedical EngineeringRaman spectroscopy is gaining its popularity in biological fields due to its ability to obtain data without altering the sample in anyway. However, there was no method of measuring the concentration of a substance of interest in real time, which can be useful in many biological applications. The conventional spectrometer was taken out and instead I used a lock-in amplifier, which is a device that amplifies signals obtained at a given frequency. This way we manipulated a method to obtain data at 1000 Hz. Moreover, noise at frequencies not equal to 1000 Hz will naturally die out due to no amplification. However, noise obtained from the laser frequencies (auto-fluorescence from sample, random scattering etc.) need to be flattened to avoid amplifying these noises. We have used HPLC grade ethanol and obtained a quantified graph to prove this idea.ope

Relative Hypoglycemia in Diabetic Patients With Critical Illness

Objectives: Relative hypoglycemia is a decrease in glucose greater than or equal to 30% below prehospital admission levels (estimated by hemoglobin A1C) but not to absolute hypoglycemia levels. It is a recognized pathophysiologic phenomenon in ambulant poorly controlled diabetic patients but remains unexamined during critical illness. We examined the frequency, characteristics, and outcome associations of relative hypoglycemia in diabetic patients with critical illness. Design: Retrospective cohort study. Setting: ICU of a tertiary hospital. Patients: One-thousand five-hundred ninety-two critically ill diabetic patients between January 2013 and December 2017. Interventions: None. Measurements and Main Results: The median age of patients was 67 years (interquartile range, 60-75 yr). The median Acute Physiology and Chronic Health Evaluation III score was 53 (interquartile range, 40-68). Thirty-four percent of patients with diabetes experienced relative hypoglycemia (exposure) during their ICU admission. Such patients had higher glycemic lability, hemoglobin A1C levels, and Acute Physiology and Chronic Health Evaluation III scores. The hazard ratio for 28-day mortality of diabetic patients, censored at hospital discharge, for patients with relative hypoglycemia was 1.9 (95% CI, 1.3-2.8) and was essentially unchanged after adjustment for episodes of absolute hypoglycemia. After an episode of relative hypoglycemia, the hazard ratio for subsequent absolute hypoglycemia in the ICU was 3.5 (95% CI, 2.3-5.3). Conclusions: In ICU patients with diabetes, relative hypoglycemia is common, increases with higher hemoglobin A1C levels, and is a modifiable risk factor for both mortality and subsequent absolute hypoglycemia. These findings provide the rationale for future interventional studies to explore new blood glucose management strategies and to substantiate the clinical relevance of relative hypoglycemia

Regulation of ADAMTS-1, -4 and -5 expression in human macrophages: differential regulation by key cytokines implicated in atherosclerosis and novel synergism between TL1A and IL-17

Atherosclerosis is an inflammatory disease of the vasculature regulated by cytokines. Macrophages play a crucial role at all stages of this disease, including regulation of foam cell formation, the inflammatory response and stability of atherosclerotic plaques. For example, matrix metalloproteinases produced by macrophages play an important role in modulating plaque stability. More recently, the ADAMTS proteases, which are known to play a key role in the control of cartilage degradation during arthritis, have been found to be expressed in atherosclerotic lesions and suggested to have potentially important functions in the control of plaque stability. Unfortunately, the action of cytokines on the expression of ADAMTS family in macrophages is poorly understood. We have investigated the effect of classical cytokines (IFN-γ and TGF-β) and those that have been recently identified (TL1A and IL-17) on the expression of ADAMTS-1, -4 and -5 in human macrophages. The expression of all three ADAMTS members was induced during differentiation of monocytes into macrophages. TGF-β had a differential action with induction of ADAMTS-1 and -5 expression and attenuation in the levels of ADAMTS-4. In contrast, IFN-γ suppressed the expression of ADAMTS-1 without having an effect on ADAMTS-4 and -5. Although TL-1A or IL-17A alone had little effect on the expression of all the members, they induced their expression synergistically when present together. These studies provide new insight into the regulation of key ADAMTS family members in human macrophages by major cytokines in relation to atherosclerosis

Effect of Indocyanine Green and Illumination on Gene Expression in Human Retinal Pigment Epithelial Cells

PURPOSE. To investigate the biological effects of indocyanine green (ICG) and acute illumination on human retinal pigment epithelial (RPE) cells. METHODS. Three concentrations (0, 0.25, and 2.5 mg/mL) of ICG were applied to ARPE19 cells for 1 minute. After isotonic rinsing, the cells were irradiated with a light beam with a wavelength spectrum from 400 to 800 nm and an output of 1850 lumens for 15 minutes. The cells were collected at timed intervals for the investigation of cell death and expression of stress-response genes by reverse transcription-polymerase chain reaction, immunofluorescence, and Western blot analysis. RESULTS. After ICG incubation, photoreactive changes were observed in the RPE cells. A reduction in cellular viability and considerable shrinkage of the cells were observed. The expressions of the apoptosis-related genes p53 and bax and the cell cycle arrest protein p21 were upregulated in cells treated with both ICG and light. Of the early-response genes, the expression of c-fos was specifically enhanced by light, with additive effects from the presence of ICG. Such stimulatory effects on these gene expressions were greater at 2.5 mg/mL than at 0.25 mg/mL ICG. CONCLUSIONS. ICG in the presence of acute illumination can elicit cell-cycle arrest and even apoptosis in RPE cells. The establishment of a safety level in the application of ICG in the region of 0.25 mg/mL is recommended. (Invest Ophthalmol Vis Sci

Identification of genes expressed by immune cells of the colon that are regulated by colorectal cancer-associated variants.

A locus on human chromosome 11q23 tagged by marker rs3802842 was associated with colorectal cancer (CRC) in a genome-wide association study; this finding has been replicated in case-control studies worldwide. In order to identify biologic factors at this locus that are related to the etiopathology of CRC, we used microarray-based target selection methods, coupled to next-generation sequencing, to study 103 kb at the 11q23 locus. We genotyped 369 putative variants from 1,030 patients with CRC (cases) and 1,061 individuals without CRC (controls) from the Ontario Familial Colorectal Cancer Registry. Two previously uncharacterized genes, COLCA1 and COLCA2, were found to be co-regulated genes that are transcribed from opposite strands. Expression levels of COLCA1 and COLCA2 transcripts correlate with rs3802842 genotypes. In colon tissues, COLCA1 co-localizes with crystalloid granules of eosinophils and granular organelles of mast cells, neutrophils, macrophages, dendritic cells and differentiated myeloid-derived cell lines. COLCA2 is present in the cytoplasm of normal epithelial, immune and other cell lineages, as well as tumor cells. Tissue microarray analysis demonstrates the association of rs3802842 with lymphocyte density in the lamina propria (p = 0.014) and levels of COLCA1 in the lamina propria (p = 0.00016) and COLCA2 (tumor cells, p = 0.0041 and lamina propria, p = 6 × 10(-5)). In conclusion, genetic, expression and immunohistochemical data implicate COLCA1 and COLCA2 in the pathogenesis of colon cancer. Histologic analyses indicate the involvement of immune pathways

Emergence of off-axis equilibria in a quantum vortex gas

We experimentally study the emergence of high-energy equilibrium states in a chiral vortex gas of like-circulation vortices realized within a disk-shaped atomic Bose-Einstein condensate. In contrast to the familiar triangular Abrikosov lattice, the lowest-energy state of the superfluid in a rotating frame, we observe the formation of rotating vortex equilibria that are highly disordered and have significant energy per vortex. Experimental stirring protocols realize equilibrium states at both positive and negative absolute temperatures of the vortex gas. At sufficiently high energies the system exhibits a symmetry breaking transition, resulting in an off-axis equilibrium phase that no longer shares the symmetry of the container. By initializing vortices in a non-equilibrium distribution with sufficient energy, relaxation to equilibrium is observed within experimental timescales and an off-axis equilibrium state emerges at negative absolute temperature. The observed equilibria are in close agreement with mean field theory of the microcanonical ensemble of the vortex gas.Comment: V1: 13 pages, 6 figures, 5 in main tex