Sea ice melt and meteoric water distributions in Nares Strait, Baffin Bay, and the Canadian Arctic Archipelago

Sea ice melt (SIM), meteoric water (river runoff net precipitation), and Pacific seawater contributions to the upper waters of the Canadian Arctic Archipelago (CAA), Nares Strait, and Baffin Bay during late summer 1997 and 2003 are estimated from salinity, δ18O, and nutrient data. Salinity-δ18O relationships within the study area suggest that the CAA inherits a net sea-ice formation (brine) signal from the Arctic Ocean. Inherited brine complicates the estimation of local contributions from sea ice melt and glacial runoff, especially where a significant component of the surface water derives from Arctic outflow. Our data are characterized by two linear relationships between salinity and δ18O, reflecting: (1) the mixing of deeper Atlantic seawater with brine-enriched halocline water of shelf origin and (2) mixing of halocline water with shallower waters freshened by meteoric water and local SIM. Inventories of Pacific water, meteoric water, net SIM, and local SIM were computed over the upper 150 m of the water column. Positive local SIM fractions were ubiquitous during late summer, with the largest inventories (\u3e1 m) found on the eastern sides of Baffin Bay, Kennedy Channel, and Davis Strait. In the CAA and Baffin Bay, freshwater inventories were dominated by contributions from meteoric and Pacific water, with little input from local SIM. In Smith Sound, where comparable data were collected in 1997 and 2003, meteoric water inventories of 8–10 m were similar for both years, whereas the Pacific water inventory was substantially lower in 2003 (\u3c80 m) than in 1997 (\u3e100 m), implying that the export of meteoric water from the Arctic Ocean is decoupled from Pacific water outflow

HST/WFC3 Observations of an Off-Nuclear Superbubble in Arp 220

We present a high spatial resolution optical and infrared study of the circumnuclear region in Arp 220, a late-stage galaxy merger. Narrowband imaging using HST/WFC3 has resolved the previously observed peak in H$\alpha$+[NII] emission into a bubble-shaped feature. This feature measures 1.6" in diameter, or 600 pc, and is only 1" northwest of the western nucleus. The bubble is aligned with the western nucleus and the large-scale outflow axis seen in X-rays. We explore several possibilities for the bubble origin, including a jet or outflow from a hidden active galactic nucleus (AGN), outflows from high levels of star formation within the few hundred pc nuclear gas disk, or an ultraluminous X-ray source. An obscured AGN or high levels of star formation within the inner $\sim$100 pc of the nuclei are favored based on the alignment of the bubble and energetics arguments.Comment: Accepted for publication in ApJ. 12 pages, 10 figure

Total versus superficial parotidectomy for stage III melanoma

BackgroundThe primary purpose of this study was to describe the parotid recurrence rates after superficial and total parotidectomy.MethodsA retrospective cohort study was performed on patients with cutaneous melanoma metastatic to the parotid gland who underwent parotidectomy from 1998 through 2014. Primary outcome was parotid bed recurrence. Secondary outcomes were facial nerve function postoperatively and at last follow‐up.ResultsOne hundred twenty‐nine patients were included in the study. Thirty‐four patients (26%) underwent a total parotidectomy and 95 patients underwent superficial parotidectomy. Twelve patients (13%) developed parotid bed recurrence after superficial parotidectomy alone versus zero after total parotidectomy (P = .035). Facial nerve function, clinically detected disease, stage, and adjuvant treatment were not statistically different between the groups (P = .32, .32, .13, and 0.99, respectively).ConclusionParotid bed melanoma recurrence was more common after superficial parotidectomy compared to total parotidectomy, and recurrence resulted in significant facial nerve functional deficit. Our results support total parotidectomy when metastatic melanoma involves the parotid nodal basin.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137735/1/hed24810_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137735/2/hed24810.pd

Neuroimmune mechanisms of opioid-mediated conditioned immunomodulation

Morphine administration elicits pronounced effects on the immune system, including decreases in natural killer (NK) cell activity and lymphocyte mitogenic responsiveness. These immune alterations can become conditioned to environmental stimuli that predict morphine as a result of Pavlovian conditioning processes. Prior work in our laboratory has shown that acute morphine exposure produces dopamine-dependent reductions of NK cell activity that are mediated peripherally by neuropeptide Y Y1 receptors. The present study examined the involvement of dopamine D1 and neuropeptide Y Y1 receptors in the conditioned immunomodulatory effects of morphine. Rats received two conditioning sessions during which an injection of morphine was paired with a distinctive environment which served as the conditioned stimulus (CS). The results show that systemic administration of the D1 antagonist SCH-23390 prior to CS re-exposure prevented the conditioned suppression of splenic NK activity but did not alter conditioned decreases in mitogen-induced lymphocyte proliferation. Furthermore, bilateral microinjections of SCH-23390 directly into the nucleus accumbens shell fully blocked conditioned changes in NK activity. In a subsequent manipulation, subcutaneous injection of the Y1 receptor antagonist BIBP3226 prior to CS re-exposure was also shown to prevent conditioned effects on NK activity. Collectively, these findings provide evidence that the nucleus accumbens shell plays an important role in conditioned immunomodulation and further suggest that the conditioned and unconditioned immunomodulatory effects of opioids involve similar receptor mechanisms

Oxidation state governs structural transitions in peroxiredoxin II that correlate with cell cycle arrest and recovery

Inactivation of eukaryotic 2-Cys peroxiredoxins (Prxs) by hyperoxidation has been proposed to promote accumulation of hydrogen peroxide (H2O2) for redox-dependent signaling events. We examined the oxidation and oligomeric states of PrxI and -II in epithelial cells during mitogenic signaling and in response to fluxes of H2O2. During normal mitogenic signaling, hyperoxidation of PrxI and -II was not detected. In contrast, H2O2-dependent cell cycle arrest was correlated with hyperoxidation of PrxII, which resulted in quantitative recruitment of ∼66- and ∼140-kD PrxII complexes into large filamentous oligomers. Expression of cyclin D1 and cell proliferation did not resume until PrxII-SO2H was reduced and native PrxII complexes were regenerated. Ectopic expression of PrxI or -II increased Prx-SO2H levels in response to oxidant exposure and failed to protect cells from arrest. We propose a model in which Prxs function as peroxide dosimeters in subcellular processes that involve redox cycling, with hyperoxidation controlling structural transitions that alert cells of perturbations in peroxide homeostasis

Sagittal Bone Saw With Orbital Blade Motion for Improved Cutting Efficiency

Sagittal bone saws are used by orthopedic surgeons for resection of bone; for example in total joint arthroplasty of the hip and knee. In order to prevent damage to surrounding tissue, sagittal saw blades typically oscillate through a small angle, resulting in reduced cutting rates due to short stroke lengths. To improve bone cutting efficiency, sagittal saws oscillate at high speeds, but this creates frictional heating that can harm bone cells. The focus of this research was to design a new sagittal sawing device for improved cutting efficiency. It was hypothesized that the addition of an impulsive thrust force during the cutting stroke would increase cutting rates in cortical bone. A cam-driven device was developed and tested in bovine cortical bone. The impulsive thrust force was achieved by creating a component of blade motion perpendicular to the cutting direction, i.e., orbital blade motion. At the start of each cutting stroke, the mechanism drove the saw blade into the surface of the bone, increasing the thrust force with the intention of increasing the depth of cut per tooth. As each cutting stroke was completed, the blade was retracted from the surface for the purpose of clearing bone chips. The design parameters investigated were cutting stroke length, thrust stroke length, and blade oscillation frequency. A three-factor, two-level design of experiments approach was used to simultaneously test for the effect of design parameters and their interactions on volumetric cutting rate (n ¼ 32). The addition of orbital blade motion to the sagittal saw improved bone cutting rates over traditional oscillatory motion, especially at lower cutting stroke lengths and higher oscillation frequencies (p < 0.05). However, the magnitude of orbital blade motion based on thrust stroke length was limited by a threshold value of approximately 0.10 mm that when exceeded caused the sagittal saw to rebound from the surface of the bone causing erratic cutting conditions. The factor with the greatest positive effect on cutting rate was oscillation frequency. Cutting rates in cortical bone can be improved with the proposed orbital action sagittal saw