Validation of a novel instrumentation (FlexOmega system) measuring oar bending moments on-water in rowing

Quantifying rowing performance can facilitate control of training load or assessment of skill level. Accordingly, the FlexOmega system was developed, which records the bending moment of the oar. This work aimed to validate this new instrumentation during a dynamic load case. Two force profiles were first derived from bending moments acquired during on-water rowing (one at race pace, one at training pace). These force profiles were then used to repeatedly load the instrumented oar on a newly developed test bench. To ultimately elaborate how precision and accuracy determined on the test bench affects everyday training, i.e., whether practitioners can reasonably use the FlexOmega system, the measurement variability observed on the test bench was related to the measurement variability seen for on-water measurements. On the test bench (featuring a mean precision of 99% and mean accuracy of 95%), a mean error of 3 Nm (mean precision: 98%, mean accuracy: 97%) was determined for the FlexOmega system for the force profile A characterised by bending moments of up to 300 Nm (racing simulated, 37 strokes per minute). For the force profile B with lower stroke rate and less force (21 strokes per minute, up to 150 Nm), the mean error was 2 Nm (mean precision: 98%, mean accuracy: 97%). The measurement variability observed on the test bench was on average for the two force profiles 30% (profile A) and 15% (profile B) of the measurement variability that occurred during on-water rowing. We conclude that improving the measurement characteristics of the instrumentation would hardly result in any practical benefit as on-water measurements seem mainly to be influenced by the rower’s skill level and environmental condition. Thus, the FlexOmega system can be used to control training intensity or to evaluate rowing performance. In addition, the presented approach for elaborating measurement characteristics could contribute to

Influence of Mechanical Unloading on Articular Chondrocyte Dedifferentiation.

Due to the limited self-repair capacity of articular cartilage, the surgical restoration of defective cartilage remains a major clinical challenge. The cell-based approach, which is known as autologous chondrocyte transplantation (ACT), has limited success, presumably because the chondrocytes acquire a fibroblast-like phenotype in monolayer culture. This unwanted dedifferentiation process is typically addressed by using three-dimensional scaffolds, pellet culture, and/or the application of exogenous factors. Alternative mechanical unloading approaches are suggested to be beneficial in preserving the chondrocyte phenotype. In this study, we examined if the random positioning machine (RPM) could be used to expand chondrocytes in vitro such that they maintain their phenotype. Bovine chondrocytes were exposed to (a) eight days in static monolayer culture; (b) two days in static monolayer culture, followed by six days of RPM exposure; and, (c) eight days of RPM exposure. Furthermore, the experiment was also conducted with the application of 20 mM gadolinium, which is a nonspecific ion-channel blocker. The results revealed that the chondrocyte phenotype is preserved when chondrocytes go into suspension and aggregate to cell clusters. Exposure to RPM rotation alone does not preserve the chondrocyte phenotype. Interestingly, the gene expression (mRNA) of the mechanosensitive ion channel decreased with progressing dedifferentiation. In contrast, the gene expression (mRNA) of the mechanosensitive ion channel was reduced around fivefold to 10-fold in all of the conditions. The application of gadolinium had only a minor influence on the results. This and previous studies suggest that the chondrocyte phenotype is preserved if cells maintain a round morphology and that the ion channel could play a key role in the dedifferentiation process

A sentinel protein assay for simultaneously quantifying cellular processes

We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput

TRPC6 in simulated microgravity of intervertebral disc cells

Purpose Prolonged bed rest and microgravity in space cause intervertebral disc (IVD) degeneration. However, the underlying molecular mechanisms are not completely understood. Transient receptor potential canonical (TRPC) channels are implicated in mechanosensing of several tissues, but are poorly explored in IVDs. Methods Primary human IVD cells from surgical biopsies composed of both annulus fibrosus and nucleus pulposus (passage 1–2) were exposed to simulated microgravity and to the TRPC channel inhibitor SKF-96365 (SKF) for up to 5 days. Proliferative capacity, cell cycle distribution, senescence and TRPC channel expression were analyzed. Results Both simulated microgravity and TRPC channel antagonism reduced the proliferative capacity of IVD cells and induced senescence. While significant changes in cell cycle distributions (reduction in G1 and accumulation in G2/M) were observed upon SKF treatment, the effect was small upon 3 days of simulated microgravity. Finally, downregulation of TRPC6 was shown under simulated microgravity. Conclusions Simulated microgravity and TRPC channel inhibition both led to reduced proliferation and increased senescence. Furthermore, simulated microgravity reduced TRPC6 expression. IVD cell senescence and mechanotransduction may hence potentially be regulated by TRPC6 expression. This study thus reveals promising targets for future studies.ISSN:0940-6719ISSN:1432-0932ISSN:00199115