The measured relaxation rates in the sham-operated animals.

<p>The figures presented are mean values of areas in both hemispheres and the six time points. No systematic variation across the hemispheres, between the time points or between the animals was observed. These values agree closely with the relaxation data measured in the contralateral hemispheres of the stroke animals as well.</p

Correlation between changes detected by RAFF and other relaxation times.

<p>The data are pooled from all time points from the five brain regions studied. The regression lines (dashed, R² values shown) show high correlations for all parameter pairs; however, the pair T₂-RAFF is the best match to the line of unity (solid line), confirming the similarity of the time courses.</p

Time courses for relative ipsi-contra differences in T₂-filtered ZAPISM MT.

<p>The values of parameter S(n µs)/S(100 µs) are shown at time points one (TP1) to six (TP6) in the different brain regions S1-C3. The results from Student’s t-test are given by: p<0.05 =  † and p<0.01 =  ‡.</p

Representative diffusion (D_av), T₂, T_1ρ and T_RAFF maps and CW-MT and ZAPI MR images.

<p>Data from one animal acquired at 1 and 5 hours post MCAO. Ischemic lesion is visible in the left hemisphere.</p

Progression of the MT changes for conventional MT and ZAPISM.

<p>Conventional MT (marked CW) is shown at offsets of 10 and 5 kHz, and ZAPISM (marked AP) on resonance, with full MT (τ  = 100 µs) and with T₂ filtering (τ  = 80, 60 and 40 µs). The values are (ipsi-contra)/contra changes at brain regions S1-C3, at time points one (TP1) to six (TP6). The results from Student’s t-tests are given by: p<0.05 =  † and p<0.01 =  ‡.</p

The temporal evolution of the relative differences in relaxation rates.

<p>Data are shown from time point one (TP1) to six (TP6) between the hemispheres in the five brain regions (S1-C3) studied. The results from Student’s t-tests are given below the graphs: p<0.05 =  † and p<0.01 =  ‡.</p

MRI relaxation time constant maps

Zipped folder containing MRI relaxation time constant maps. Files are meant to be opened and analyzed using the Aedes toolbox for MATLAB. See the publication in PLOS ONE for more details. Files are titled "MRI_[mouse identifier]_[MRI sequence].t1r [or .t1]". Mice 13021, 13224, 13255, N3, N4, and N5 are mice with MPS I; mice 13257, 13258, N1, N2, and N6 are heterozygotes

MRI analysis_for Dryad

Excel spreadsheet containing analyzed MRI data. The “MRI ROI comparison” tab contains mean relaxation time constants for each ROI in each animal for each sequence, as well as means for each genotype. The “MRI vs histology” tab contains correlation of tissue section-derived normalized optical density with MRI relaxation time constants for each MRI sequence. The “Outliers” tab contains analysis of each animal for outlying values (see the publication in PLOS ONE for details). Mice 13021, 13224, 13255, N3, N4, and N5 are mice with MPS I; mice 13257, 13258, N1, N2, and N6 are heterozygotes

Histology analysis_for Dryad

Excel spreadsheet containing analyzed histological data. The “Optical density” tab contains raw optical density values for each tissue section, normalized values, normalized means for each animal, and normalized means for each genotype. The “Outliers” tab contains analysis of each animal for outlying values (see the publication in PLOS ONE for details). The “cc thickness” tab contains raw data for corpus callosum thickness, comparison with atlas values for each section, animal means, and genotype means. Mice 13021, 13224, 13255, N3, N4, and N5 are mice with MPS I; mice 13257, 13258, N1, N2, and N6 are heterozygotes

Histology images

Zipped folder containing photomicrographs of mouse brain sections. Sections are from MPS I mice and IDUA heterozygotes, and are stained with the black gold stain. Images have been resized and converted to grayscale. Images are scaled to 173 pixels per millimeter unless noted otherwise in the filename (“..._NotToScale”). Files are titled "histology_[mouse identifier]_[tissue section identifier].tif". Mice 13021, 13224, 13255, N3, N4, and N5 are mice with MPS I; mice 13257, 13258, N1, N2, and N6 are heterozygotes