The Giant Branches of Open and Globular Clusters in the Infrared as Metallicity Indicators: A Comparison with Theory

We apply the giant branch slope-[Fe/H] relation derived by Kuchinski et al. [AJ, 109, 1131 (1995)] to a sample of open clusters. We find that the slope of the giant branch in K vs. (J-K) color-magnitude diagrams correlates with [Fe/H] for open clusters as it does for metal-rich globular clusters but that the open cluster data are systematically shifted to less negative values of giant branch slope, at constant [Fe/H]. We use isochrone models to examine the theoretical basis for this relationship and find that for a given value of [Fe/H], the slope of the relationship remains constant with decreasing population age but the relation shifts to less negative values of giant branch slope with decreasing age. Both of these theoretical predictions agree with the trends found in the data. Finally, we derive new coefficients for the giant branch slope-[Fe/H] relation for specific members of 3 populations, metal-rich globular clusters, bulge stars and open clusters.Comment: 16 pages including 3 figures (AASTEX), AJ Accepted, also available at http://www.astronomy.ohio-state.edu/~martini/pubs.htm

Uncertainties of size measurements in electron microscopy characterization of nanomaterials in foods

Electron microscopy is a recognized standard tool for nanomaterial characterization, and recommended by the European Food Safety Authority for the size measurement of nanomaterials in food. Despite this, little data have been published assessing the reliability of the method, especially for size measurement of nanomaterials characterized by a broad size distribution and/or added to food matrices. This study is a thorough investigation of the measurement uncertainty when applying electron microscopy for size measurement of engineered nanomaterials in foods. Our results show that the number of measured particles was only a minor source of measurement uncertainty for nanomaterials in food, compared to the combined influence of sampling, sample preparation prior to imaging and the image analysis. The main conclusion is that to improve the measurement reliability, care should be taken to consider replications and matrix removal prior to sample preparation

Implications of New JHK Photometry and a Deep Infrared Luminosity Function for the Galactic Bulge

We present deep near-IR photometry for Galactic bulge stars in Baade's Window, $(l,b) = (1.0\deg, -3.9\deg),$ and another minor axis field at $(l,b) = (0^\circ,-6^\circ)$. We combine our data with previously published photometry and construct a luminosity function over the range $5.5 \leq K_0 \leq 16.5$, deeper than any previously published. The slope of this luminosity function and the magnitude of the tip of the first ascent giant branch are consistent with theoretical values derived from isochrones with appropriate age and metallicity. We use the relationship between [Fe/H] and the giant branch slope derived from near-IR observations of metal rich globular clusters by Kuchinski {\it et al.} [AJ, 109, 1131 (1995)] to calculate the mean metallicity for several bulge fields along the minor axis. For Baade's Window we derive $\langle {\rm[Fe/H]}\rangle = -0.28 \pm 0.16$, consistent with the recent estimate of McWilliam \& Rich [ApJS, 91, 749 (1994)], but somewhat lower than previous estimates based on CO and TiO absorption bands and the $JHK$ colors of M giants by Frogel {\it et al.} [ApJ, 353, 494 (1990)]. Between $b = -3\deg$ and $-12\deg$ we find a gradient in $\langle {\rm [Fe/H]}\rangle$ of $-0.06 \pm 0.03$ dex/degree or $-0.43 \pm 0.21$ dex/kpc for $R_0 = 8$ kpc, consistent with other independent derivations. We derive a helium abundance for Baade's Window with the $R$ and $R^\prime$ methods and find that $Y = 0.27 \pm 0.03$ implying $\Delta Y / \Delta Z = 3.3 \pm 1.3$. Next, we find that the bolometric corrections for bulge K giants ($V - K \leq 2$) are in excellent agreement with empirical derivations based on observations of globular cluster and local field stars. However, for the redder M giants weComment: Accepted by the Astronomical Journal. 43 pages, uuencoded compressed PostScript, no figures or tables. A complete (text, figs and tables) preprint is also available at ftp://bessel.mps.ohio-state.edu/pub/terndrup/bwphot.tar.Z (compressed tar file with PostScript

Vimentin expression influences flow dependent VASP phosphorylation and regulates cell migration and proliferation

The cytoskeleton plays a central role for the integration of biochemical and biomechanical signals across the cell required for complex cellular functions. Recent studies indicate that the intermediate filament vimentin is necessary for endothelial cell morphogenesis e.g. in the context of leukocyte transmigration. Here, we present evidence, that the scaffold provided by vimentin is essential for VASP localization and PKG mediated VASP phosphorylation and thus controls endothelial cell migration and proliferation. Vimentin suppression using siRNA technique significantly decreased migration velocity by 50% (videomicroscopy), diminished transmigration activity by 42.5% (Boyden chamber) and reduced proliferation by 43% (BrdU-incorporation). In confocal microscopy Vimentin colocalized with VASP and PKG in endothelial cells. Vimentin suppression was accompanied with a translocation of VASP from focal contacts to the perinuclear region. VASP/Vimentin and PKG/Vimentin colocalization appeared to be essential for proper PKG mediated VASP phosphorylation because we detected a diminished expression of PKG and p(Ser239)-VASP in vimentin-suppressed cells, Furthermore, the induction of VASP phosphorylation in perfused arteries was markedly decreased in vimentin knockout mice compared to wildtypes. A link is proposed between vimentin, VASP phosphorylation and actin dynamics that delivers an explanation for the important role of vimentin in controlling endothelial cell morphogenesis

Distances to Populous Clusters in the LMC via the K-Band Luminosity of the Red Clump

We present results from a study of the distances and distribution of a sample of intermediate-age clusters in the Large Magellanic Cloud. Using deep near-infrared photometry obtained with ISPI on the CTIO 4m, we have measured the apparent K-band magnitude of the core helium burning red clump stars in 17 LMC clusters. We combine cluster ages and metallicities with the work of Grocholski & Sarajedini to predict each cluster's absolute K-band red clump magnitude, and thereby calculate absolute cluster distances. An analysis of these data shows that the cluster distribution is in good agreement with the thick, inclined disk geometry of the LMC, as defined by its field stars. We also find that the old globular clusters follow the same distribution, suggesting that the LMC's disk formed at about the same time as the globular clusters, ~ 13 Gyr ago. Finally, we have used our cluster distances in conjunction with the disk geometry to calculate the distance to the LMC center, for which we find (m-M)o = 18.40 +/- 0.04_{ran} +/- 0.08_{sys}, or Do = 47.9 +/- 0.9 +/- 1.8 kpc.Comment: 31 pages including 5 figures and 7 tables. Accepted for publication in the August 2007 issue of A

The Clustering of Extragalactic Extremely Red Objects

We have measured the angular and spatial clustering of 671 K5 Extremely Red Objects (EROs) from a 0.98 square degree sub-region of the NOAO Deep Wide-Field Survey (NDWFS). Our study covers nearly 5 times the area and has twice the sample size of any previous ERO clustering study. The wide field of view and BwRIK passbands of the NDWFS allow us to place improved constraints on the clustering of z=1 EROs. We find the angular clustering of EROs is slightly weaker than in previous measurements, and w(1')=0.25+/-0.05 for K<18.40 EROs. We find no significant correlation of ERO spatial clustering with redshift, apparent color or absolute magnitude, although given the uncertainties, such correlations remain plausible. We find the spatial clustering of K5 EROs is well approximated by a power-law, with r_0=9.7+/-1.1 Mpc/h in comoving coordinates. This is comparable to the clustering of 4L* early-type galaxies at z<1, and is consistent with the brightest EROs being the progenitors of the most massive ellipticals. There is evidence of the angular clustering of EROs decreasing with increasing apparent magnitude, when NDWFS measurements of ERO clustering are combined with those from the literature. Unless the redshift distribution of K>20 EROs is very broad, the spatial clustering of EROs decreases from r_0=9.7+/-1.1 Mpc/h for K20 EROs.Comment: Accepted for publication in the ApJ. 29 pages with 10 figures. The NOAO Deep Wide-Field Survey Bootes data release is available online at http://www.noao.edu/noao/noaodeep