Strigolactone alleviates the salinity-alkalinity stress of Malus hupehensis seedlings

Salinity-alkalinity stress can remarkably affect the growth and yield of apple. Strigolactone (SL) is a class of carotenoid-derived compounds that functions in stress tolerance. However, the effects and mechanism of exogenous SL on the salinity-alkalinity tolerance of apple seedlings remain unclear. Here, we assessed the effect of SL on the salinity-alkalinity stress response of Malus hupehensis seedlings. Results showed that treatment with 100 μM exogenous SL analog (GR24) could effectively alleviate salinity-alkalinity stress with higher chlorophyll content and photosynthetic rate than the apple seedlings without GR24 treatment. The mechanism was also explored: First, exogenous GR24 regulated the expression of Na+/K+ transporter genes and decreased the ratio of Na+/K+ in the cytoplasm to maintain ion homeostasis. Second, exogenous GR24 increased the enzyme activities of superoxide, peroxidase and catalase, thereby eliminating reactive oxygen species production. Third, exogenous GR24 alleviated the high pH stress by regulating the expression of H+-ATPase genes and inducing the production of organic acid. Last, exogenous GR24 application increased endogenous acetic acid, abscisic acid, zeatin riboside, and GA3 contents for co-responding to salinity-alkalinity stress indirectly. This study will provide important theoretical basis for analyzing the mechanism of exogenous GR24 in improving salinity-alkalinity tolerance of apple

Editorial: Advances in Aquaculture Ecology Research

This Special Issue describes the advances in the last decades in the research fields of individual ecology of commercial aquatic organisms, the ecology of aquaculture systems, interactions between aquaculture activities and the environment, the structure and function of the microbial community, principles of environment management in aquaculture ecosystems, etc [...

Effects of the Amplitude and Frequency of Salinity Fluctuations on the Growth Performance of Juvenile Tongue Sole (Cynoglossus emilaevis)

The effects of amplitude (2, 4, 6, 8 ppt) and frequency (2, 4, 8 days) of salinity fluctuations on the growth, body composition, and energy budget in juvenile tongue sole (Cynoglossus semilaevis) were investigated for 64 days. Results showed that the frequency and amplitude, as well as the interaction between them, significantly affected the specific growth rate. The tongue sole had higher specific growth rates at the amplitudes of 4 and 6 ppt and frequencies of 4 and 8 days than tongue sole in other treatments or the unfluctuating control. In these treatments, food consumption, food conversion efficiency, and apparent digestion in terms of energy were also significantly higher than in the control. The growth rate of the juvenile tongue sole was a quadratic function of the salinity amplitude at various frequencies and was described by the equation G = β0 + β1(SA) + β2(SA)2, where G represents the specific growth rate on a 64-day basis, SA is salinity amplitude in ppt, β0 is the intercept on the G axis, and β1 and β2 are the regression coefficients, respectively. The optimal salinity amplitudes for the best growth at salinity fluctuation frequencies of 2, 4, and 8 days were estimated to be 3.54, 4.89, and 4.74 ppt, respectively, suggesting that commercial farmers can rear juvenile tongue sole in moderate salinity fluctuations to achieve better growth performance

Genetic adaptation of microbial populations present in high-intensity catfish production systems with therapeutic oxytetracycline treatment

Microbial communities that are present in aquaculture production systems play significant roles in degrading organic matter, controlling diseases, and formation of antibiotic resistance. It is important to understand the diversity and abundance of microbial communities and their genetic adaptations associated with environmental physical and chemical changes. Here we collected water and sediment samples from a high-intensity catfish production system and its original water reservoir. The metagenomic analysis showed that Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, and Firmicutes were the top five phyla identified from all samples. The aquaculture production system significantly changed the structure of aquatic microbial populations. Substantial changes were also observed in SNP patterns among four sample types. The gene-specific sweep was found to be more common than genome-wide sweep. The selective sweep analysis revealed that 21 antibiotic resistant (AR) genes were under selection, with most belonging to antibiotic efflux pathways. Over 200 AR gene gains and losses were determined by changes in gene frequencies. Most of the AR genes were characterized as ABC efflux pumps, RND efflux pumps, and tetracycline MFS efflux pumps. Results of this study suggested that aquaculture waste, especially waste containing therapeutic antibiotics, has a significant impact on microbial population structures and their genetic structures

Genetic adaptation of microbial populations present in high-intensity catfish production systems with therapeutic oxytetracycline treatment

Abstract Microbial communities that are present in aquaculture production systems play significant roles in degrading organic matter, controlling diseases, and formation of antibiotic resistance. It is important to understand the diversity and abundance of microbial communities and their genetic adaptations associated with environmental physical and chemical changes. Here we collected water and sediment samples from a high-intensity catfish production system and its original water reservoir. The metagenomic analysis showed that Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, and Firmicutes were the top five phyla identified from all samples. The aquaculture production system significantly changed the structure of aquatic microbial populations. Substantial changes were also observed in SNP patterns among four sample types. The gene-specific sweep was found to be more common than genome-wide sweep. The selective sweep analysis revealed that 21 antibiotic resistant (AR) genes were under selection, with most belonging to antibiotic efflux pathways. Over 200 AR gene gains and losses were determined by changes in gene frequencies. Most of the AR genes were characterized as ABC efflux pumps, RND efflux pumps, and tetracycline MFS efflux pumps. Results of this study suggested that aquaculture waste, especially waste containing therapeutic antibiotics, has a significant impact on microbial population structures and their genetic structures

Effects of Acute and Chronic Heavy Metal (Cu, Cd, and Zn) Exposure on Sea Cucumbers (Apostichopus japonicus)

Acute and chronic toxicity tests were conducted with sea cucumber (Apostichopus japonicus) exposed to heavy metals. Acute toxicity values (96 h LC50) were 2.697, 0.133, and 1.574 mg L−1 for Zn, Cu, and Cd, respectively, and were ranked in order of toxicity: Cu > Cd > Zn. Under chronic metal exposure the specific growth rates of sea cucumbers decreased with the increase of metal concentration for all the three metals. After acute metal exposure, the oxygen consumption rate (OCR) decreased. The OCRs in all groups were significantly different than control (P<0.05) except in the group treated with 1.00 mg L−1 Zn (P<0.05), where the increase of OCR was observed. The OCRs in groups chronically exposed to metals were significantly lower than that in the control group (P<0.05). The activity of both pyruvate kinase (PK) and hexokinase (HK) in sea cucumbers followed: respiratory tree > muscle > intestine in natural sea water. After chronic Zn, Cu, and Cd exposure, the change pattern of HK and PK in respiratory tree, muscle, and intestine varied slightly. However, the activity of the enzyme showed a general trend of increase and then decrease and the higher the exposure concentration was, the earlier the highest point of enzyme activity was obtained

The Boundary Proportion Differential Control Method of Micro-Deformable Manipulator with Compensator Based on Partial Differential Equation Dynamic Model

It is challenging to accurately judge the actual end position of the manipulator—regarded as a rigid body—due to the influence of micro-deformation. Its precise and efficient control is a crucial problem. To solve the problem, the Hamilton principle was used to establish the partial differential equation (PDE) dynamic model of the manipulator system based on the infinite dimension of the working environment interference and the manipulator space. Hence, it resolves the common overflow instability problem in the micro-deformable manipulator system modeling. Furthermore, an infinite-dimensional radial basis function neural network compensator suitable for the dynamic model was proposed to compensate for boundary and uncertain external interference. Based on this compensation method, a distributed boundary proportional differential control method was designed to improve control accuracy and speed. The effectiveness of the proposed model and method was verified by theoretical analysis, numerical simulation, and experimental verification. The results show that the proposed method can effectively improve the response speed while ensuring accuracy

The Effects of Different Carbon Sources on the Production Environment and Breeding Parameters of Litopenaeus vannamei

This study investigated the effect of different carbon sources on water quality, ammonia removal pathways, the bacterial community, and the production of Litopenaeus vannamei in outdoor culture tanks. Three systems were established: a clear water system (CW) and biofloc technology (BFT) systems with added molasses (M-BF) or poly (3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) (P-BF). The average pH, total alkalinity, total organic carbon, biofloc volume, chlorophyll a, nitrite, nitrate, total nitrogen, and nitrification rate were significantly different among the treatments. Microbial composition varied and different dominant taxa were identified in the treatments by linear discriminant analysis effect size. Redundancy analysis indicated that the water quality parameters affected the distribution of the microbial community. Moreover, the genus Leucothrix was closely related to the M-BF treatment. Chemoheterotrophy and aerobic chemoheterotrophy were the most abundant functions in all treatments. A comparison of functions using BugBase indicated that the relative abundance of several functions such as biofilm formation, stress tolerance and functions related to anaerobic processes increased in the M-BF treatment. The specific growth rate, growth rate, and survival rate of shrimp were significantly higher in the P-BF system than in the CW system and the feed conversion ratio in the BFT treatments was significantly lower than that in the CW system. Overall, adding carbon sources affected water quality, microbial community, and shrimp performance. The results show that PHBV is a good alternative to carbon sources

Experimental Study of Indoor Air Distribution and Thermal Environment in a Ceiling Cooling Room with Mixing Ventilation, Underfloor Air Distribution and Stratum Ventilation

A ceiling cooling system integrated with a mechanical ventilation system has been widely used in modern buildings with large sensible cooling loads due to the high thermal comfort level and large energy efficiency. However, there is a lack of systematic research on the influence factors such as ceiling surface temperature and cooling load on the indoor air distribution and thermal environment, and the impact of ventilation system type in the ceiling cooling room is still unclear. Therefore, this paper presented an experimental study of indoor air distribution and thermal environment in a ceiling cooling (CC) room with mixing ventilation (MV), underfloor air distribution (UFAD) and stratum ventilation (SV); the ceiling surface temperature was 17 °C–26 °C and the internal or external cooling load was 41.5 W/m2–69.5 W/m2. The results showed that the vertical air temperature difference and contaminant removal effectiveness were 0.2 °C–0.4 °C and 0.53–0.85 with CC + MV, 0 °C–1.2 °C and 0.68–1.25 with CC + UFAD and 0.3 °C–0.9 °C and 0.50–0.83 with CC + SV, and the corresponding heat removal effectiveness and air diffusion performance index were 0.96–1.11 and 96–100%, 0.9–1.5 and 57–100% and 1.11–1.34 and 71–100%, respectively. Moreover, the difference between mean radiant temperature and air temperature and the predicted mean vote of thermal sensation were from 0 °C to 0.9 °C and between 0 and 0.26 with CC + MV, from −0.1 °C to 2.2 °C and between −0.1 and 0.42 with CC + UFAD and from −0.1 °C to 0.9 °C and between −0.2 and 0.13 with CC + SV. Hence, the ventilation system type clearly affected the indoor air distribution and thermal environment in the ceiling cooling room, and the experimental results would be beneficial for the design and control of a ceiling cooling system combined with a mechanical ventilation system in practice