First report of Mesocriconema sphaerocephalum (Taylor, 1936) Loof, 1989 associated with carrot (Daucus carota subsp. Stativus) in Vietnam

Our study recorded the presence of Mesocriconema sphaeroce phalum on carrot in Hanoi city and Hai Duong province in Vietnam. This species was identified by morphometric, morphological characterizations, and molecular characterization of D2D3 of 28S rDNA sequence. To our knowledge, this is the first report of M. sphaerocephalum on carrot in Vietnam

ADSORPTION OF MERCURIC ION FROM AQUEOUS SOLUTIONS USING MODIFIED FLY ASH

In this study, fly ash was treated with NaOH solution (FAN) before modifying with (3-mercaptopropyl) triethoxysilane - MPTMS (FAMPS). By using FTIR, FESEM, XRD, EDX, and BET techniques, the change in structure, composition and morphology of FAN and FAMPS was evaluated. The FTIR spectra of FAN and FAMPS showed that there is no chemical reaction between the MPTMS and FAN. After modification, the FAMPS has a rough surface with composition difference from the FAN. Mercuric ion adsorption behavior as well as adsorptionisotherm models (Langmuir and Freundlich) of the FAN and FAMPS were also investigated and discussed. Thanks to FAN modification, the mercuric ion removal percent of the FAMPS was higher than that of the FAN. Owing to the adsorption data, Freundlich isotherm modelwas fitted for the mercuric ion adsorption process

Preliminary establishment of a multiplex PCR method for the identification of pork and beef meat based on cytochrome-B Gene

Molecular species detection in food has become common in the last years. In this study, multiplex PCR (mPCR) technique was applied to discriminate between the pork and beef with the aim of detecting the pork in food products made from beef. We developed a m-PCR protocol detected the presence of pork in food products made from beef based on Cytochrome-b gene by a general primer pair F and primer pair (RP, RC), specific two private with pig and cow. The m-PCR method was successfully designed with technical parameters, such as the annealing temperature of 590C and the final concentration of each primer in a reaction of 0,4µM. The minimum DNA concentration of pig and cow could be detected by m-PCR, which was 0,1ng/µl. This process was tested on 24 different beef sausage samples tagged no pork, resulting in 11/24 (46%) of samples, were found the presence of pork and 54% (13/24) of samples no beef and pork. According to this sequencing result that are completely accordant, we affirm primer-specific amplification in this study can be applied to experiment on large number of sample and the ton other types food made from beef

ĐIỀU TRA TÌNH HÌNH CỎ LỒNG VỰC PHÁT SINH TRỞ LẠI TRÊN RUỘNG LÚA GIEO SẠ SAU KHI SỬ DỤNG THUỐC TRỪ CỎ Ở THỪA THIÊN HUẾ

Studying herbicide resistance in Vietnam is limited, and there are very few reports on the regrowth of weed after applying herbicides. A survey to investigate the cultivating habit and the barnyard grass regrowing in the direct-seeded rice fields after applying herbicides in Thua Thien Hue shows that the main labourers of rice cultivation are male with a low educational level to understand scientific and technical knowledge. The most common grass regrowing in direct-seeded rice fields after applying herbicides includes Barnyard grass (Echinochloa crus-galli), Grass-like fimbry (Fimbristylis miliacea), Bermuda grass (Cynodon dactylon), and Sprangletop (Leptochloa chinensis). The yield decreases from 12.1 to 14.0% because of regrowing of barnyard grass. The herbicides commonly used in Thua Thien Hue are Sofic 300EC (Pretilachlor) and Sunrice 15WDG (Ethoxysulfuron), but their effectiveness has decreased in recent time owing to farmers’ inappropriate spraying skills and the decrease in the effectiveness of herbicides. A more thorough assessment of the factors that cause grass regrowing after spraying herbicides is required for better weed management.Nghiên cứu sự kháng thuốc trừ cỏ ở Việt Nam còn hạn chế và có rất ít báo cáo về sự phát sinh của cỏ dại sau khi sử dụng thuốc trừ cỏ. Kết quả điều tra về tập quán canh tác và tình hình cỏ lồng vực phát sinh trở lại trên ruộng lúa sau khi sử dụng thuốc trừ cỏ ở Thừa Thiên Huế cho thấy lực lượng lao động chính canh tác lúa là nam giới, trình độ dân trí không cao nên khả năng tiếp thu kiến thức khoa học – kỹ thuật hạn chế. Các loại cỏ gây hại phổ biến trên ruộng lúa gieo sạ là cỏ lồng vực (Echinochloa crus-galli), cỏ chác (Fimbristylis miliacea), cỏ chỉ (Cynodon dactylon), cỏ đuôi phụng (Leptochloa chinensis) là các loại cỏ xuất hiện trở lại sau khi đã sử dụng thuốc trừ cỏ. Cỏ lồng vực mọc trở lại có thể gây giảm năng suất cây lúa 12,1–14,0%. Các loại thuốc trừ cỏ được sử dụng phổ biến là Sofic 300EC (Pretilachlor) và Sunrice 15WDG (Ethoxysulfuron). Hiệu quả thuốc trừ cỏ lúa có xu hướng giảm trong thời gian gần đây do kỹ thuật sử dụng và sự giảm hiệu quả của các sản phẩm thuốc trừ cỏ. Chúng tôi kiến nghị cần đánh giá đầy đủ hơn về các yếu tố gây nên tình trạng cỏ dại mọc trở lại sau khi phun thuốc để có định hướng quản lý cỏ dại tốt hơn

Determination of the Administration Routes, Doses and Appropriate Age to Vaccinate With Ornitin Triple Vaccine For Cross-breed Colored Broilers in Vietnam

The study was to determine the appropriate dose and administration route of Ornitin Triple vaccine in cross-breed coloured broilers in Vietnam by evaluating the antibody titer against Ornithobacterium rhinotracheale (ORT) and local reactions at injection sites on chickens after vaccination. The study was divided into 2 trials. Both trials were designed with 3 vaccine dose groups: 0.0ml (control group), 0.25ml and 0.5ml and 2 different administration routes: subcutaneous at neck (SC) and intramuscular at breast (IM) injection. The result showed that, no statistically significant difference was found between antibody titer of two administration routes as well as 2 vaccine doses until 13-week-old. Local reactions at the injection sites of IM route was less severe than SC at neck and in higher dose would produce a more severe swelling reaction. Daily weight gain was found to have a slight decrease in the vaccinated groups within 2 weeks after vaccination, however, no statistically significant difference was found in later stage (P > 0.05). In conclusion, Ornitin Triple can be used to vaccinate by IM with the dose of 0.25ml for coloured broilers at early age (3-week-old), or 0.5ml for older birds and should be careful for some reactions at the injection sites

Synthesis of new simplified hemiasterlin derivatives with α,β-unsaturated carbonyl moiety.

International audienceIn this Letter, we report a convenient and efficient method for the synthesis of new simplified derivatives of hemiasterlin in which the α,α-dimethylbenzylic moiety A is replaced by α,β-unsaturated aryl groups as Michael acceptor. Most of these derivatives have a strong cytotoxic activity on three human tumor cell lines (KB, Hep-G2 and MCF7). Analogs 17b and 17f showed a high cytotoxicity against KB and Hep-G2 cancer cell lines comparable to paclitaxel and ellipticine

Effects of oil and grape seed tannin extract on intakes, digestibility, milk yield and composition of Saanen goats

An experiment was conducted as a 4×4 Latin square design using 4 lactating Saanen goats, 19 months old and 47.9±1.04 kg of body weight, to evaluate the effect of oil and grape seed tannin extract (GSTE) supplementation on feed intake, digestibility, milk yield and milk composition. Each experimental period lasted for 21 days including 16 days for adjustment and 5 days for sampling. Goats were fed a control diet (Ctrl) consisting of 60% concentrate and 40% fresh Para grass (dry matter, DM, basis) while other 3 treatments were supplementation of 2.5% soybean oil (SO); 2.5% soybean oil + tuna fish oil at 3:2 w:w (SFO); 2.5% soybean oil + tuna fish oil at 3:2 w:w + 0.8% GSTE (OCT). The results showed that oil and GSTE did not affect feed intake, digestibility, milk yield and composition of goats (P > 0.05). However, digestibility of EE was higher (P < 0.05) in SFO and OCT diets (85.4% and 84.7%, respectively) compared with Ctrl (76.2%). Combined data suggested that feeding 2.5% oil blend with or without 0.8% GSTE increased EE digestibility in goats without affecting intake, animal performance and milk composition