8,694 research outputs found
The application of the scanning electron microscope to studies of current multiplication, avalanche breakdown and thermal runaway. Part 2 - General studies, mainly non-thermal
Scanning electron microscope applications in study of current multiplication, avalanche breakdown, and thermal runaway - Nonthermal effects in Read diode
Time Domain Simulations of Arm Locking in LISA
Arm locking is a technique that has been proposed for reducing laser
frequency fluctuations in the Laser Interferometer Space Antenna (LISA), a
gravitational-wave observatory sensitive in the milliHertz frequency band. Arm
locking takes advantage of the geometric stability of the triangular
constellation of three spacecraft that comprise LISA to provide a frequency
reference with a stability in the LISA measurement band that exceeds that
available from a standard reference such as an optical cavity or molecular
absorption line. We have implemented a time-domain simulation of arm locking
including the expected limiting noise sources (shot noise, clock noise,
spacecraft jitter noise, and residual laser frequency noise). The effect of
imperfect a priori knowledge of the LISA heterodyne frequencies and the
associated 'pulling' of an arm locked laser is included. We find that our
implementation meets requirements both on the noise and dynamic range of the
laser frequency.Comment: Revised to address reviewer comments. Accepted by Phys. Rev.
Implicating Receptor Activator of NF-κB (RANK)/RANK Ligand Signalling in Microglial Responses to Toll-Like Receptor Stimuli
Inflammation in the perinatal brain caused by maternal or intrauterine fetal infection is now well established as an important contributor to the development of perinatal brain injury. Exposure to inflammatory products can impair perinatal brain development and act as a risk factor for neurological dysfunction, cognitive disorders, cerebral palsy, or preterm birth. Pre-exposure to inflammation significantly exacerbates brain injury caused by hypoxic/ischaemic insult. Tumour necrosis factor (TNF) is a family of cytokines largely involved in inflammation signalling. In our previous study, we identified the importance of TNF-related apoptosis-inducing ligand (TRAIL) signalling in the development of perinatal brain injury. We observed a significant increase in the expression levels of a soluble decoy receptor for TRAIL, osteoprotegerin (OPG). Besides TRAIL, OPG is able to bind the receptor activator of the NF-&#x03BA;B (RANK) ligand (RANKL) and inhibit its signalling. The function of the RANK/RANKL/OPG system in the brain has not come under much scrutiny. The aim of this research study was to elucidate the role of RANK, RANKL, and OPG in microglial responses to the proinflammatory stimuli lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (Poly I:C). Here, we show that RANK signalling is important for regulating the activation of the BV2 microglial cell line. We found that LPS treatment causes a significant decrease in the expression of RANK in the BV2 cell line while significantly increasing the expression of OPG, Toll-like receptor (TLR)3, and the adaptor proteins MyD88 and TRIF. We found that pretreatment of BV2 cells with RANKL for 24 h before the LPS or Poly I:C exposure decreases the expression of inflammatory markers such as inducible nitric oxide synthase and cyclooxygenase. This is accompanied by a decreased expression of the TLR adaptor proteins MyD88 and TRIF, which we observed after RANKL treatment. Similar results were obtained in our experiments with primary mouse microglia. Using recently developed CRISPR/Cas9 technology, we generated a BV2 cell line lacking RANK (RANK<sup>-/-</sup> BV2). We showed that most effects of RANKL pretreatment were abolished, thereby proving the specificity of this effect. Taken together, these findings suggest that RANK signalling is important for modulating the inflammatory activation of microglial cells to a moderate level, and that RANK attenuates TLR3/TLR4 signalling.</jats:p
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Factors influencing the utilisation of free-standing and alongside midwifery units in England: a qualitative research study
OBJECTIVE: To identify factors influencing the provision, utilisation and sustainability of midwifery units (MUs) in England.
DESIGN: Case studies, using individual interviews and focus groups, in six National Health Service (NHS) Trust maternity services in England.
SETTING AND PARTICIPANTS: NHS maternity services in different geographical areas of England Maternity care staff and service users from six NHS Trusts: two Trusts where more than 20% of all women gave birth in MUs, two Trusts where less than 10% of all women gave birth in MUs and two Trusts without MUs. Obstetric, midwifery and neonatal clinical leaders, managers, service user representatives and commissioners were individually interviewed (n=57). Twenty-six focus groups were undertaken with midwives (n=60) and service users (n=52).
MAIN OUTCOME MEASURES: Factors influencing MU use.
FINDINGS: The study findings identify several barriers to the uptake of MUs. Within a context of a history of obstetric-led provision and lack of decision-maker awareness of the clinical and economic evidence, most Trust managers and clinicians do not regard their MU provision as being as important as their obstetric unit (OU) provision. Therefore, it does not get embedded as an equal and parallel component in the Trust's overall maternity package of care. The analysis illuminates how implementation of complex interventions in health services is influenced by a range of factors including the medicalisation of childbirth, perceived financial constraints, adequate leadership and institutional norms protecting the status quo.
CONCLUSIONS: There are significant obstacles to MUs reaching their full potential, especially free-standing midwifery units. These include the lack of commitment by providers to embed MUs as an essential service provision alongside their OUs, an absence of leadership to drive through these changes and the capacity and willingness of providers to address women's information needs. If these remain unaddressed, childbearing women's access to MUs will continue to be restricted
Stress in frictionless granular material: Adaptive Network Simulations
We present a minimalistic approach to simulations of force transmission
through granular systems. We start from a configuration containing cohesive
(tensile) contact forces and use an adaptive procedure to find the stable
configuration with no tensile contact forces. The procedure works by
sequentially removing and adding individual contacts between adjacent beads,
while the bead positions are not modified. In a series of two-dimensional
realizations, the resulting force networks are shown to satisfy a linear
constraint among the three components of average stress, as anticipated by
recent theories. The coefficients in the linear constraint remain nearly
constant for a range of shear loadings up to about .6 of the normal loading.
The spatial distribution of contact forces shows strong concentration along
``force chains". The probability of contact forces of magnitude f shows an
exponential falloff with f. The response to a local perturbing force is
concentrated along two characteristic rays directed downward and laterally.Comment: 8 pages, 8 figure
Force distributions in 3D granular assemblies: Effects of packing order and inter-particle friction
We present a systematic investigation of the distribution of normal forces at
the boundaries of static packings of spheres. A new method for the efficient
construction of large hexagonal-close-packed crystals is introduced and used to
study the effect of spatial ordering on the distribution of forces. Under
uniaxial compression we find that the form for the probability distribution of
normal forces between particles does not depend strongly on crystallinity or
inter-particle friction. In all cases the distribution decays exponentially at
large forces and shows a plateau or possibly a small peak near the average
force but does not tend to zero at small forces.Comment: 9 pages including 8 figure
Origin of broad luminescence from site‐controlled InGaN nanodots fabricated by selective‐area epitaxy
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106777/1/pssa201330362.pd
Stresses in isostatic granular systems and emergence of force chains
Progress is reported on several questions that bedevil understanding of
granular systems: (i) are the stress equations elliptic, parabolic or
hyperbolic? (ii) how can the often-observed force chains be predicted from a
first-principles continuous theory? (iii) How to relate insight from isostatic
systems to general packings? Explicit equations are derived for the stress
components in two dimensions including the dependence on the local structure.
The equations are shown to be hyperbolic and their general solutions, as well
as the Green function, are found. It is shown that the solutions give rise to
force chains and the explicit dependence of the force chains trajectories and
magnitudes on the local geometry is predicted. Direct experimental tests of the
predictions are proposed. Finally, a framework is proposed to relate the
analysis to non-isostatic and more realistic granular assemblies.Comment: 4 pages, 2 figures, Corrected typos and clkearer text, submitted to
Phys. Rev. Let
Chemical and Thermal Analysis
Work has included significant research in several areas aimed at further clarification of the aging and chemical failure mechanism of thermoplastics (PVDF or Tefzel) for pipes. Among the areas investigated were the crystallinity changes associated with both the Coflon and Tefzel after various simulated environmental exposures using X-Ray diffraction analysis. We have found that significant changes in polymer crystallinity levels occur as a function of the exposures. These crystallinity changes may have important consequences on the fracture, fatigue, tensile, and chemical resistance of the materials. We have also noted changes in the molecular weight distribution of the Coflon material using a dual detector Gel Permeation Analysis. Again these changes may result in variation in the mechanical and chemical properties in the material. We conducted numerous analytical studies with methods including X-Ray Diffraction, Gel Permeation Chromatography, Fourier Transform Infrared Spectroscopy, Thermogravimetric Analysis, and Differential Scanning Calorimetry. We investigated a number of aged samples of both Tefzel and Coflon that were forwarded from MERL. Pressurized tests were performed in a modified Fluid G, which we will call G2. In this case the ethylene diamine concentration was increased to 3 percent in methanol. Coflon pipe sections and powdered Coflon were exposed in pressure cells at 1700 psi at three separate test temperatures, 70 C, 110 C, and 130 C. The primary purpose of the pressure tests in Fluid G2 was to further elucidate the aging mechanism of PVDF degradation
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