Deliverable D5.6 Final LinkedTV End-to-End Platform

This Deliverable describes the final LinkedTV End-to-End Platform, which integrates a whole workflow from video ingestion over video analysis, annotated media fragment generation, content enrichment to personalized playout by a dedicated media player

Deliverable D5.3 First LinkedTV End-to-end Platform

This Deliverable describes the first version of the LinkedTV end-to-end platform, which intergrates a whole workflow from video ingestion over video analysis, annotated media fragment generation, content enrichment to personalized playout by a dedicated media player

Deliverable D5.7 Validation of the LinkedTV Architecture

The LinkedTV architecture lays the foundation for the LinkedTV system. It consists of the integrating platform for the end-to-end functionality, the backend components and the supporting client components. Since the architecture of a software system has a fundamental impact on quality attributes, it is important to evaluate its design. The document at hand reports on the validation of the LinkedTV architecture

Deliverable D5.1 LinkedTV Platform and Architecture

The objective of Linked TV is the integration of hyperlinks in videos to open up new possibilities for an interactive, seamless usage of video on the Web. LinkedTV provides a platform for the automatic identification of media fragments, their metadata annotations and connection with the Linked Open Data Cloud, which enables to develop applications for the search for objects, persons or events in videos and retrieval of more detailed related information. The objective of D5.1 is the design of the platform architecture for the server and client side based on the requirements derived from the scenarios defined in WP6 and technical needs from WPs 1-4. The document defines workflows, components, data structures and tools. Flexible interfaces and an efficient communications infrastructure allow for a seamless deployment of the system in heterogeneous, distributed environments. The resulting design builds the basis for the distributed development of all components in WP1-4 and their integration into a platform enabling for the efficient development of Hypervideo applications

Combined local and systemic immunization is essential for durable T-cell mediated heterosubtypic immunity against influenza A virus

The threat from unpredictable influenza virus pandemics necessitates the development of a new type of influenza vaccine. Since the internal proteins are highly conserved, induction of T cells targeting these antigens may provide the solution. Indeed, adenoviral (Ad) vectors expressing flu nucleoprotein have previously been found to induce short-term protection in mice. In this study we confirm that systemic (subcutaneous (s.c.) immunization rapidly induced heterosubtypic protection predominantly mediated by CD8 T cells, but within three months clinical protection completely disappeared. Local (intranasal (i.n.)) immunization elicited delayed, but more lasting protection despite relatively inefficient immunization. However, by far, the most robust protection was induced by simultaneous, combined (i.n. + s.c.) vaccination, and, notably, in this case clinical protection lasted at least 8 months without showing any evidence of fading. Interestingly, the superior ability of the latter group to resist reinfection correlated with a higher number of antigen-specific CD8 T cells in the spleen. Thus, detailed analysis of the underlying CD8 T cell responses highlights the importance of T cells already positioned in the lungs prior to challenge, but at the same time underscores an important back-up role for circulating antigen-specific cells with the capacity to expand and infiltrate the infected lungs

Towards an improvement of optically stimulated luminescence (OSL) age uncertainties:Modelling OSL ages with systematic errors, stratigraphic constraints and radiocarbon ages using the R package BayLum

International audienceAbstract. Statistical analysis has become increasingly important in optically stimulated luminescence (OSL) dating since it has become possible to measure signals at the single-grain scale. The accuracy of large chronological datasets can benefit from the inclusion, in chronological modelling, of stratigraphic constraints and shared systematic errors. Recently, a number of Bayesian models have been developed for OSL age calculation; the R package “BayLum” presented herein allows different models of this type to be implemented, particularly for samples in stratigraphic order which share systematic errors. We first show how to introduce stratigraphic constraints in BayLum; then, we focus on the construction, based on measurement uncertainties, of dose covariance matrices to account for systematic errors specific to OSL dating. The nature (systematic versus random) of errors affecting OSL ages is discussed, based – as an example – on the dose rate determination procedure at the IRAMAT-CRP2A laboratory (Bordeaux). The effects of the stratigraphic constraints and dose covariance matrices are illustrated on example datasets. In particular, the benefit of combining the modelling of systematic errors with independent ages, unaffected by these errors, is demonstrated. Finally, we discuss other common ways of estimating dose rates and how they may be taken into account in the covariance matrix by other potential users and laboratories. Test datasets are provided as a Supplement to the reader, together with an R markdown tutorial allowing the reproduction of all calculations and figures presented in this study

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags.

RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.BACKGROUND: Knowledge of the structure of gene expression is essential for mammalian transcriptomics research. We analyzed a collection of more than one million porcine expressed sequence tags (ESTs), of which two-thirds were generated in the Sino-Danish Pig Genome Project and one-third are from public databases. The Sino-Danish ESTs were generated from one normalized and 97 non-normalized cDNA libraries representing 35 different tissues and three developmental stages. RESULTS: Using the Distiller package, the ESTs were assembled to roughly 48,000 contigs and 73,000 singletons, of which approximately 25% have a high confidence match to UniProt. Approximately 6,000 new porcine gene clusters were identified. Expression analysis based on the non-normalized libraries resulted in the following findings. The distribution of cluster sizes is scaling invariant. Brain and testes are among the tissues with the greatest number of different expressed genes, whereas tissues with more specialized function, such as developing liver, have fewer expressed genes. There are at least 65 high confidence housekeeping gene candidates and 876 cDNA library-specific gene candidates. We identified differential expression of genes between different tissues, in particular brain/spinal cord, and found patterns of correlation between genes that share expression in pairs of libraries. Finally, there was remarkable agreement in expression between specialized tissues according to Gene Ontology categories. CONCLUSION: This EST collection, the largest to date in pig, represents an essential resource for annotation, comparative genomics, assembly of the pig genome sequence, and further porcine transcription studies.Published versio