CORTWNVMS_forDryad_Gervasi_6_9_17

Individual sheets for analyses of survival, viremia, cytokine levels, host performance, tolerance, and infectiousnes

Map of the endemic area and locations of the entomological and serological surveys carried out between 1995 and 2008.

<p>1- Playa de Oro, 2 - Angostura, 3 -Guayabal, 4 -Agua Salada, 5 -Pichiyacu, 6 - Playa Grande, 7- Herradura, 8 -San Miguel, 9- El Tigre, 10-Agua Blanca, 11- Callemansa, 12 - Corriente Grande, 13 – Colón de Onzole, 14 - Calpulí, 15 - Las Pavas, 16- Naranjal.</p

Entomological assessment 1995, 2000, 2004 and 2008, in the major collection sites located in three major river systems in the onchocerciasis endemic zone of Ecuador.

<p>No. =  Number of black flies analyzed; IR =  infectivity rate in fly heads (per 2000); CI =  Confidence Interval; ND =  Not done;</p><p>* =  treatment was stopped in this particular river system prior to this date.</p

The prevalence of anti-<i>Onchocerca volvulus</i> antibodies (Ov-16 antigen) in 1–15 year old children resident in the community collection sites in the onchocerciasis endemic area of Ecuador.

<p>Samples taken in 2001 and 2008.</p><p>Rounds  =  The number of rounds of treatment received; Total number present  =  Total number of children within the tested age range present in the community; NA =  not available</p

Drug distribution coverage for the Onchocerciasis Program.

<p>Percentage (%) of eligible people treated every 6 months from 1991–2009. Line indicates a 85% coverage level.</p

The prevalence of anti-<i>Onchocerca volvulus</i> antibodies (Ov-16 antigen) in 1-16 year old children resident in various communities within the onchocerciasis endemic area of Ecuador.

<p>Samples taken in 2006 and 2007.</p><p>Rounds  =  The number of rounds of treatment received; Total number present  =  Total number of children within the tested age range present in the community; NA =  not available.</p

Additional file 1: of Evidence of suppression of onchocerciasis transmission in the Venezuelan Amazonian focus

Detailed description of study area, criteria for elimination, calculation of infection indices and additional entomological data. Text S1. Geographical and environmental characteristics of the Venezuelan part of the Amazonian onchocerciasis focus. Text S2. Endemic communities, mapping and geographical information system. Text S3. World Health Organization (WHO) criteria for onchocerciasis elimination. Text S4. Calculation of parasitological indices. Text S5. Calculation of transmission indices. Table S1. Number of ivermectin rounds for twice yearly (6-monthly) and quarterly (3-monthly) treatments that reached a therapeutic coverage ≥85 %, by geographical sub-area in the Venezuelan part of the Amazonian focus. Figure S1. Monthly biting rates of Simulium guianense s.l. recorded in Orinoquito and Parima B during baseline (1982–2000) entomological evaluations. Figure S2. Infectivity rates and mean nos. of L3 larvae per fly for S. guianense s.l. in Orinoquito and Parima B during baseline (1982–2000) entomological evaluations. Figure S3. Monthly infective biting rates and monthly transmission potentials for S. guianense s.l. in Orinoquito and Parima B during baseline (1982–2000) entomological evaluations. Figure S4. Relationship between the proportion of infective flies and the mean number of infective larvae per fly in S. guianense s.l. at baseline. Table S2. Comparison of manual dissection and PCR (using PoolScreen™) for estimation of infectivity rates in S. guianense s.l., Koyowë, May 2000. (DOCX 129 kb