11 research outputs found
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Overcoming EGFR T790M and C797S resistance with mutant-selective allosteric inhibitors
EGFR tyrosine kinase inhibitors (TKIs) gefitinib, erlotinib and afatinib are approved treatments for non-small cell lung cancers harboring activating mutations in the EGFR kinase1,2, but resistance arises rapidly, most frequently due to the secondary T790M mutation within the ATP-site of the receptor.3,4 Recently developed mutant-selective irreversible inhibitors are highly active against the T790M mutant5,6, but their efficacy can be compromised by acquired mutation of C797, the cysteine residue with which they form a key covalent bond7. All current EGFR TKIs target the ATP-site of the kinase, highlighting the need for therapeutic agents with alternate mechanisms of action. Here we describe rational discovery of EAI045, an allosteric inhibitor that targets selected drug-resistant EGFR mutants but spares the wild type receptor. A crystal structure shows that the compound binds an allosteric site created by the displacement of the regulatory C-helix in an inactive conformation of the kinase. The compound inhibits L858R/T790M-mutant EGFR with low-nanomolar potency in biochemical assays, but as a single agent is not effective in blocking EGFR-driven proliferation in cells due to differential potency on the two subunits of the dimeric receptor, which interact in an asymmetric manner in the active state8. We observe dramatic synergy of EAI045 with cetuximab, an antibody therapeutic that blocks EGFR dimerization9,10, rendering the kinase uniformly susceptible to the allosteric agent. EAI045 in combination with cetuximab is effective in mouse models of lung cancer driven by L858R/T790M EGFR and by L858R/T790M/C797S EGFR, a mutant that is resistant to all currently available EGFR TKIs. More generally, our findings illustrate the utility of purposefully targeting allosteric sites to obtain mutant-selective inhibitors
Design, synthesis, and biological evaluation of simplified alpha-keto heterocycle, trifluoromethyl ketone, and formyl substituted folate analogues as potential inhibitors of GAR transformylase and AICAR transformylase
A series of simplified alpha-keto heterocycle, trifluoromethyl ketone, and formyl substituted folate analogues lacking the benzoylglutamate subunit were prepared and examined as potential inhibitors of glycinamide ribonucleotide transformylase (GAR Tfase) and aminoimidazole carboxamide transformylase (AICAR Tfase)
Identification and Characterization of Small Molecule Modulators of the Epstein–Barr Virus-Induced Gene 2 (EBI2) Receptor
Oxysterols have recently been identified
as natural ligands for
a G protein-coupled receptor called EBI2 (aka GPR183) (Nature 2011, 475, 524; 519). EBI2 is highly
expressed in immune cells (J. Biol. Chem. 2006, 281, 13199), and its activation has been shown to be
critical for the adaptive immune response and has been genetically
linked to autoimmune diseases such as type I diabetes (Nature 2010, 467, 460). Here we describe the
isolation of a potent small molecule antagonist for the EBI2 receptor.
First, we identified a small molecule agonist NIBR51 (<b>1</b>), which enabled identification of inhibitors of receptor activation.
One antagonist called NIBR127 (<b>2</b>) was used as a starting
point for a medicinal chemistry campaign, which yielded NIBR189 (<b>4m</b>). This compound was extensively characterized in binding
and various functional signaling assays. Furthermore, we have used <b>4m</b> to block migration of a monocyte cell line called U937,
suggesting a functional role of the oxysterol/EBI2 pathway in these
immune cells
Discovery of (<i>R</i>,<i>E</i>)‑<i>N</i>‑(7-Chloro-1-(1-[4-(dimethylamino)but-2-enoyl]azepan-3-yl)‑1<i>H</i>‑benzo[<i>d</i>]imidazol-2-yl)-2-methylisonicotinamide (EGF816), a Novel, Potent, and WT Sparing Covalent Inhibitor of Oncogenic (L858R, ex19del) and Resistant (T790M) EGFR Mutants for the Treatment of EGFR Mutant Non-Small-Cell Lung Cancers
Over the past decade, first and second
generation EGFR inhibitors have significantly improved outcomes for
lung cancer patients with activating mutations in EGFR. However, both
resistance through a secondary T790M mutation at the gatekeeper residue
and dose-limiting toxicities from wild-type (WT) EGFR inhibition ultimately
limit the full potential of these therapies to control mutant EGFR-driven
tumors and new therapies are urgently needed. Herein, we describe
our approach toward the discovery of <b>47</b> (EGF816, nazartinib), a novel,
covalent mutant-selective EGFR inhibitor with equipotent activity
on both oncogenic and T790M-resistant EGFR mutations. Through molecular
docking studies we converted a mutant-selective high-throughput screening
hit (<b>7</b>) into a number of targeted covalent EGFR inhibitors
with equipotent activity across mutants EGFR and good WT-EGFR selectivity.
We used an abbreviated in vivo efficacy study for prioritizing compounds with good tolerability
and efficacy that ultimately led to the selection of <b>47</b> as the clinical candidate
Synthesis, Structure–Activity Relationships, and in Vivo Efficacy of the Novel Potent and Selective Anaplastic Lymphoma Kinase (ALK) Inhibitor 5‑Chloro‑<i>N</i>2‑(2-isopropoxy-5-methyl-4-(piperidin-4-yl)phenyl)‑<i>N</i>4‑(2-(isopropylsulfonyl)phenyl)pyrimidine-2,4-diamine (LDK378) Currently in Phase 1 and Phase 2 Clinical Trials
The synthesis, preclinical profile,
and in vivo efficacy in rat xenograft models of the novel and selective
anaplastic lymphoma kinase inhibitor <b>15b</b> (LDK378) are
described. In this initial report, preliminary structure–activity
relationships (SARs) are described as well as the rational design
strategy employed to overcome the development deficiencies of the
first generation ALK inhibitor <b>4</b> (TAE684). Compound <b>15b</b> is currently in phase 1 and phase 2 clinical trials with
substantial antitumor activity being observed in ALK-positive cancer
patients