Comparative evaluation of the QIAsymphony RGQ system with the easyMAG/R-gene combination for the quantitation of cytomegalovirus DNA load in whole blood

Abstract Background The detection of cytomegalovirus (CMV) DNA in blood is a key feature of the virological surveillance of immunocompromised patients. Methods The QIAsymphony RGQ system (QIAGEN S.A.S., France) combines the extraction/distribution steps on QIAsymphony SP/AS instruments with amplification on a Rotor-Gene Q RT-PCR machine. This system was compared to a strategy combining an extraction step on the NUCLISENS easyMAG platform (bioMérieux) with the CMV R-gene kit (Argene) on 100 whole blood specimens collected from immunocompromised patients of the University Hospital of Saint-Etienne, France. Results The overall agreement between the two strategies was 86% (kappa coefficient of 0.67); the 14 discrepant results corresponded to low DNA loads. The 62 samples found positive with both tests were correlated (Pearson r coefficient of 0.70, P 10 copies/ml with the easyMAG/Argene strategy (P 10 copies/ml. The inter-run and intra-run variability was consistently lower with the QIAGEN platform. Conclusions These results validate the performance of the QIAsymphony RGQ system for the routine quantitation of CMV DNA. This fully-automated platform reduces the hands-on time and improves standardization, traceability and quality control assessment.</p

Modulation of oxidative stress and microinflammatory status by colloids in refractory dialytic hypotension

<p>Abstract</p> <p>Background</p> <p>Intradialytic hypotension may adversely affect the outcome of chronic hemodialysis. Therapeutic albumin has powerful anti-oxidant and anti-inflammatory properties. We have recently shown that systematic colloid infusion during hemodialysis sessions improves hemodynamic parameters in most dialysis hypotension-prone patients unresponsive to usual of preventive measures.</p> <p>We postulated that frequent hypotensive episodes may lead to a noxious inflammatory response mediated by oxidative stress induced by ischemia-reperfusion. The aim of this study was therefore to analyze the effect of 20% albumin and 4% gelatin infusions on oxidative stress and microinflammatory status in hypotension-prone patients unresponsive to usual preventive measures.</p> <p>Methods</p> <p>Prospective cross-over study (lasting 20 weeks) of routine infusion of 200 ml of 20% albumin versus 200 ml of 4% gelatin in 10 patients with refractory intradialytic hypotension. We analyzed the effect of 20% albumin and 4% gelatin on microinflammatory status, oxidative stress, serum nitrite and nitrate levels by analysis of variance.</p> <p>Results</p> <p>A significant decrease in serum ceruloplasmin and serum C3 was observed during the albumin period (p < 0.05, repeated measure ANOVA). A significant decrease in serum hydrogen peroxide was seen during albumin and gelatin administration (p < 0.01, repeated measure ANOVA) and a very large decrease in serum lipid peroxides was observed during the albumin period only (p < 0.01, Friedman test). Serum lactoferrin, serum proinflammatory cytokines and serum nitrite and nitrate levels remained stable during the different periods of this pilot trial.</p> <p>Conclusions</p> <p>We conclude that the improvement in microinflammatory status observed during colloid infusion in hypotension-prone dialysis patients may be related to a decrease in ischemia-reperfusion of noble organs, together with a specific reduction in oxidative stress by albumin.</p> <p>Trial registration</p> <p><a href="http://www.controlled-trials.com/ISRCTN20957055">ISRCTN 20957055</a></p

Les articles de Paris sous l’œil de l’expert : le problème des alliages à base de cuivre et d’étain au Moyen Âge (XIIIe-XIVe s.)

International audienc

Les métiers du cuivre à Paris vers 1300 : Topographie et étude sociale

International audienc

Etude de la diversité génétique du gène de la protéine GP41 de souches HIV-1 de sous-types B et de CRF02_AG isolées de patients n'ayant jamais été traités par enfuvirtide

LYON1-BU Santé (693882101) / SudocSudocFranceF

La ville neuve du Temple, Paris au Moyen Âge : recherches récentes

National audienc

Comparative Evaluation of a Commercially Available Automated System for Extraction of Viral DNA from Whole Blood: Application to Monitoring of Epstein-Barr Virus and Cytomegalovirus Load ▿

The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% for EBV and 87.2% for CMV

Infection par le virus de l’immunodéficience humaine

Le GALT (gut associated lymphoid tissue) est à l’origine de nombreuses anomalies immunologiques au cours de l’infection par le virus de l’immunodéficience humaine de type 1 (VIH-1). Il constitue un réservoir majeur pour ce virus, dont la persistance, malgré le traitement antirétroviral, constitue un obstacle majeur à la guérison des patients. Il est également impliqué dans l’état d’hyperactivation immunitaire systémique observé chez les sujets infectés. Le GALT est le siège d’une déplétion précoce et massive de lymphocytes T CD4+ et d’une réplication virale qui conduit à une altération de la muqueuse intestinale favorisant les phénomènes de translocation microbienne et donc, à terme, à une progression de la maladie. Cette revue se propose de faire le point sur le rôle du GALT au cours de l’infection par le VIH ainsi que sur les différentes options thérapeutiques actuelles visant à réduire le réservoir viral digestif et préserver une immunité muqueuse compétente au niveau intestinal. Limiter la taille du réservoir viral du GALT est en effet devenu primordial chez les patients infectés par le VIH, dans l’objectif de pouvoir, un jour, arrêter le traitement antirétroviral sans risquer une reprise de la réplication virale