Dataset of seed infestation rates sampled on 32 sites from 1977 to 2010.

These data were entirely used for the descriptive analysis and partially used for parameter estimation. We indicated the year and the site of record, the number of cones sampled, the total number of seeds in cones, the number of parasitized seeds in cones, the global seed infestation rate, the total number of M. pinsapinis emerged, the total number of M. schimitscheki emerged, the contribution of M. pinsapinis and M. schimitscheki to the global SIR. The total number of adults emerged was observed from 1999 to 2007 and was estimated from diapause pattern for cohorts from 2008 to 2010

Microsatellite-data

This file corresponds to the genotypes of the individual analysed in the paper. All were collected in the field

Sample locations of the 16 <i>M. schimitscheki</i> populations used in this study.

<p>Informations associated with the site codes below are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070818#pone-0070818-t001" target="_blank">Table 1</a>.</p

Genetic clustering of ten successive cohorts of <i>M. schimitscheki</i> (1999–2008).

<p>This Bayesian analysis implemented in STRUCTURE <b>used</b> a model allowing admixture and assumed two population clusters (K = 2). A: Graphical representation of the two genetic clusters, where each vertical line represents an individual and each color represents a cluster. Individuals are grouped by year of cohort production and diapause phenotype (SD = 2-year diapause, PD = 4-year prolonged diapause). B: Curve of Evanno's DeltaK corresponding to the STRUCTURE simulations. C: Assignment frequencies of individuals to each cluster in each group (in green and red according to the colors of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070818#pone-0070818-g003" target="_blank">Figure 3a</a>). Frequencies of admixed individuals are in blue.</p

Pairwise Fst divergence between successive cohorts of a natural invasive population of <i>M. schimitscheki</i> in southeastern France.

<p>Sample size for each genotyped cohort is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070818#pone-0070818-t002" target="_blank">Table 2</a>. The 2000 cohort was excluded due to low sample size (N = 5). Pairwise Fst matrix was obtained using all microsatellite loci after applying the correction for null alleles implemented in GENEPOP. Fst values in bold were significantly different from 0 (P<0.05).</p

Propensity to prolonged diapause and indices of population genetics of ten successive cohorts (1999–20008) in a natural invasive population of <i>M. schimitscheki</i> collected at Mont Ventoux, France.

<p>SD: individuals (males and females) emerging after the obligatory 2-year diapause; 4-year PD: individuals (males and females) emerging after a 4-year prolonged diapause; N: number of genotyped females in the cohort; Na: average number of alleles; He: expected heterozygosity; Ho: observed heterozygosity; AR: allelic richness (minimum sample size of 5); PAR: private allelic richness estimated after a rarefaction procedure; St (P): probabilities associated with the rejection of the mutation–drift equilibrium using a sign test at the 0.05 threshold (probabilities lower than 0.05 are in bold).</p

Adult emergences from seeds of <i>C. atlantica</i> in southern French cohorts of <i>M. schimitscheki</i> over the five consecutive years following their productions (1999–2007).

<p>Emergences occurring beyond the 2-year obligatory diapause due to host cone maturation reflect propensities to prolonged diapause. Nc: number of cohorts surveyed per year of production on which mean emergence percentages and their standard errors (bars) were estimated. In 2008, only one population (Mont Ventoux) was surveyed for genetic purposes (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070818#pone-0070818-t002" target="_blank">Table 2</a>).</p

Pairwise Fst divergence between 12 groups of <i>M. schimitscheki</i> produced between 1999 and 2008, expressing either a short (2 years: SD) or a prolonged diapause (4 years: PD) phenotype.

<p>Individuals of the n-SD group were produced at a year n and emerged at year n+2. Individuals of the n-PD group were produced at a year n and emerged at year n+4. Both 2000-PD and 2002-PD groups were excluded from this analysis due to low sample sizes (N<10).This pairwise Fst matrix was obtained using eight microsatellite loci after applying the correction for null alleles implemented in GENEPOP. Fst values in bold were significantly different from 0 (P<0.05). The numbers of genotyped individuals in each group are in subscript.</p

mtDNA-Sequences-data

This file corresponds to the sequence and corresponding information on individuals analyzed in the documen

Comparison of the ontogeny of <i>Cupressus sempervirens</i> female reproductive structures with seasonal timing of the oviposition activity of the seed wasp <i>Megastigmus wachtli</i> (MW) in southeastern France.

<p><b>a</b>, Longitudinal section of an ovule collected on June 22, 2011 showing a megagametophyte at prefertilization stage with archegonial complex (star). Note the presence of pollen tubes within the nucellus. <b>b</b>, Post-fertilisation megagametophyte from an ovule collected on July 10, 2011. The early proembryo (arrow) is just emerging from the fertilized archegonium. <b>c</b>, and <b>d</b>, Sections from ovules collected on July 27 and August 24, 2011, respectively showing proembryos (arrows) within the megagametophyte tissues. CC, corrosion cavity; M, megagametophyte; N, nucellus; PT, pollen tubes. All scale bars 200 = μm. <b>e</b>, Frequencies of oviposition activity in MW; oviposition begins at female emergence. Grey bars: periods of maximal oviposition activity. <b>f</b>, Section of a parasitized <i>C</i>. <i>sempervirens</i> young seed collected on July 22, 2011 and showing a larva (arrow) within the megagametophyte tissue. M, megagametophyte; N, nucellus. Bar 200 = μm.</p