A uvbyCaHbeta CCD Analysis of the Open Cluster Standard, NGC 752

Precision uvbyCaHbeta photometry of the nearby old open cluster, NGC 752, is presented. The mosaic of CCD fields covers an area ~42' on a side with internal precision at the 0.005 to 0.010 mag level for the majority of stars down to V~15. The CCD photometry is tied to the standard system using an extensive set of published photoelectric observations adopted as secondary standards within the cluster. Multicolor indices are used to eliminate as nonmembers a large fraction of the low probability proper-motion members near the faint end of the main sequence, while identifying 24 potential dwarf members between V=15.0 and 16.5, eight of which have been noted before from Vilnius photometry. From 68 highly probable F dwarf members, we derive a reddening estimate of E(b-y)= 0.025 +/- 0.003 (E(B-V) = 0.034 +/- 0.004), where the error includes the internal photometric uncertainty and the systematic error arising from the choice of the standard (b-y), Hbeta relation. With reddening fixed, [Fe/H] is derived from the F dwarf members using both m_1 and hk, leading to [Fe/H] = -0.071 +/-0.014 (sem) and -0.017 +/- 0.008 (sem), respectively. Taking the internal precision and possible systematics in the standard relations into account, [Fe/H] for NGC 752 becomes -0.03 +/-0.02. With the reddening and metallicity defined, we use the Victoria-Regina isochrones on the Stromgren system and find an excellent match for (m-M) = 8.30 +/- 0.05 and an age of 1.45 +/- 0.05 Gyr at the appropriate metallicity.Comment: 37 pages, 11 figures, 4 tables. Accepted to Astronomical Journa

Photodynamic Therapy of Necrobiosis Lipoidica - A Multicenter Study of 18 Patients

Background: Necrobiosis lipoidica (NL) is a granulomatous skin disease of unknown origin, and no reliably effective treatment option exists to handle this often disfiguring disease. Recently, a patient with long-lasting NL was reported to be cured by topical photodynamic therapy (PDT). Objective: To evaluate the overall potential of PDT in the treatment of NL on the lower legs. Methods: Retrospective study of 18 patients (aged 16 - 62 years) from 3 European university departments of dermatology treated with PDT for NL. Methyl aminolevulinate or 5-aminolevulinic acid were used as topically applied photosensitizers. Illumination followed with red light-emitting diode light. Results: Complete response was seen in 1/18 patients after 9 PDT cycles, and partial response in 6/18 patients (2 - 14 PDT cycles) giving an overall response rate of 39% (7/18). Conclusion: Although almost 40% of the cases showed some degree of response, PDT cannot currently be recommended as first-line therapy of NL. Subpopulations of therapy-resistant NL patients may, however, benefit from PDT. Copyright (C) 2008 S. Karger AG, Base

Chronic Progressive External Ophthalmoplegia Is Associated with a Novel Mutation in the Mitochondrial tRNA(Asn) Gene

Chronic progressive external ophthalmoplegia (CPEO) is caused by a decreased oxidative phosphorylation (OXPHOS) activity due to large-scale deletions of the mitochondrial genome in 50 % of the patients. The deletions encompass structural OXPHOS genes as well as tRNA genes, required for their expression so that the pathogenesis could be due to the deleted OXPHOS subunits or to an impaired mitochondrial translation. We have analyzed the mitochondrial genome of a patient presenting with CPEO for single base substitutions and discovered a novel heteroplasmic mutation in the tRNAAsn gene at position 5692 that converts a highly conserved adenine into a guanine. This mutation is unique because it is located at the transition of the anticodon loop to the anticodon stem and it leads to an additional base pair, thus reducing the number of loop-forming nucleotides from seven to five. Our findings suggest that CPEO can be caused by a single base substition in a mitochondrial tRNA gene so that the mitochondrial protein synthesis becomes the rate limiting step in OXPHOS fidelity

Numerical solution of flow fields surrounding Saturn type vehicles

Numerical solution of flow fields surrounding Saturn type vehicle

Feed-Forward Propagation of Temporal and Rate Information between Cortical Populations during Coherent Activation in Engineered In Vitro Networks.

Transient propagation of information across neuronal assembles is thought to underlie many cognitive processes. However, the nature of the neural code that is embedded within these transmissions remains uncertain. Much of our understanding of how information is transmitted among these assemblies has been derived from computational models. While these models have been instrumental in understanding these processes they often make simplifying assumptions about the biophysical properties of neurons that may influence the nature and properties expressed. To address this issue we created an in vitro analog of a feed-forward network composed of two small populations (also referred to as assemblies or layers) of living dissociated rat cortical neurons. The populations were separated by, and communicated through, a microelectromechanical systems (MEMS) device containing a strip of microscale tunnels. Delayed culturing of one population in the first layer followed by the second a few days later induced the unidirectional growth of axons through the microtunnels resulting in a primarily feed-forward communication between these two small neural populations. In this study we systematically manipulated the number of tunnels that connected each layer and hence, the number of axons providing communication between those populations. We then assess the effect of reducing the number of tunnels has upon the properties of between-layer communication capacity and fidelity of neural transmission among spike trains transmitted across and within layers. We show evidence based on Victor-Purpura's and van Rossum's spike train similarity metrics supporting the presence of both rate and temporal information embedded within these transmissions whose fidelity increased during communication both between and within layers when the number of tunnels are increased. We also provide evidence reinforcing the role of synchronized activity upon transmission fidelity during the spontaneous synchronized network burst events that propagated between layers and highlight the potential applications of these MEMs devices as a tool for further investigation of structure and functional dynamics among neural populations

Repeating Spatial-Temporal Motifs of CA3 Activity Dependent on Engineered Inputs from Dentate Gyrus Neurons in Live Hippocampal Networks.

Anatomical and behavioral studies, and in vivo and slice electrophysiology of the hippocampus suggest specific functions of the dentate gyrus (DG) and the CA3 subregions, but the underlying activity dynamics and repeatability of information processing remains poorly understood. To approach this problem, we engineered separate living networks of the DG and CA3 neurons that develop connections through 51 tunnels for axonal communication. Growing these networks on top of an electrode array enabled us to determine whether the subregion dynamics were separable and repeatable. We found spontaneous development of polarized propagation of 80% of the activity in the native direction from DG to CA3 and different spike and burst dynamics for these subregions. Spatial-temporal differences emerged when the relationships of target CA3 activity were categorized with to the number and timing of inputs from the apposing network. Compared to times of CA3 activity when there was no recorded tunnel input, DG input led to CA3 activity bursts that were 7× more frequent, increased in amplitude and extended in temporal envelope. Logistic regression indicated that a high number of tunnel inputs predict CA3 activity with 90% sensitivity and 70% specificity. Compared to no tunnel input, patterns of >80% tunnel inputs from DG specified different patterns of first-to-fire neurons in the CA3 target well. Clustering dendrograms revealed repeating motifs of three or more patterns at up to 17 sites in CA3 that were importantly associated with specific spatial-temporal patterns of tunnel activity. The number of these motifs recorded in 3 min was significantly higher than shuffled spike activity and not seen above chance in control networks in which CA3 was apposed to CA3 or DG to DG. Together, these results demonstrate spontaneous input-dependent repeatable coding of distributed activity in CA3 networks driven by engineered inputs from DG networks. These functional configurations at measured times of activation (motifs) emerge from anatomically accurate feed-forward connections from DG through tunnels to CA3

Moist turbulent Rayleigh-Benard convection with Neumann and Dirichlet boundary conditions

Turbulent Rayleigh-Benard convection with phase changes in an extended layer between two parallel impermeable planes is studied by means of three-dimensional direct numerical simulations for Rayleigh numbers between 10^4 and 1.5\times 10^7 and for Prandtl number Pr=0.7. Two different sets of boundary conditions of temperature and total water content are compared: imposed constant amplitudes which translate into Dirichlet boundary conditions for the scalar field fluctuations about the quiescent diffusive equilibrium and constant imposed flux boundary conditions that result in Neumann boundary conditions. Moist turbulent convection is in the conditionally unstable regime throughout this study for which unsaturated air parcels are stably and saturated air parcels unstably stratified. A direct comparison of both sets of boundary conditions with the same parameters requires to start the turbulence simulations out of differently saturated equilibrium states. Similar to dry Rayleigh-Benard convection the differences in the turbulent velocity fluctuations, the cloud cover and the convective buoyancy flux decrease across the layer with increasing Rayleigh number. At the highest Rayleigh numbers the system is found in a two-layer regime, a dry cloudless and stably stratified layer with low turbulence level below a fully saturated and cloudy turbulent one which equals classical Rayleigh-Benard convection layer. Both are separated by a strong inversion that gets increasingly narrower for growing Rayleigh number.Comment: 19 pages, 13 Postscript figures, Figures 10,11,12,13, in reduced qualit