8 research outputs found
Average percent remaining activity of the mAb binding to the biotinylated synthetic peptides.
<p>The ability of human serum antibodies to bind to the mimotopic peptides selected with anti-VSG monoclonal antibodies was assessed by means of an inhibition ELISA. *: <i>p</i><0.05 and **: <i>p</i><0.01. Compared to the ten HAT negative sera, the nine HAT positive sera significantly inhibited the binding of anti-LiTat 1.5 mAb H12H3 to peptide 21, 22, 23, 28, C59 and C60 and the binding of anti-LiTat 1.3 mAb H13F7 to peptides 25, 60 and 61.</p
Amino acid sequences of peptides obtained by phage display with anti- VSG LiTat 1.5 mAb H12H3.
<p>The first sequence displayed is amino acid 265 to 288 of the protein sequence of VSG LiTat 1.5. Homologous amino acids are indicated in grey and amino acids that are identical to those of the VSG protein sequence are in bold and grey. All peptide sequences include the GGGS-spacer (connecting phage protein pIII and the random peptide in the original library) at the C-terminal. When expressed on the phage, the first cysteine of the C7C-peptides is preceded by alanine. AAA: eluted via antigen competition. PPP: eluted via acidification. Freq: number of times this sequence was found amongst seventy-nine selected phage clones. Name: name of the synthesised peptide.*: peptide selected for biotinylation. NW: not withheld. % identity: percentage identity of the peptide sequence with a stretch of 14 AA (AA 268 to 281) within the protein sequence of VSG LiTat 1.5. % RA mAb-VSG: percentage remaining of the mAb binding to its corresponding native VSG after inhibition by the synthetic peptide at 67 µg/ml.</p
SpeciesTree concatenated alignment dataset
The alignment used to generate the species tree corresponds to the concatenation of twenty-nine conserved proteins as described in the material and method section of the paper
Phylogeny of bacterial ketoacyl synthase domains
Maximum likelihood phylogenetic tree of bacterial KS based on gene clusters encoding secondary lipids biosynthesis and enediynes
C domains alignement
Alignement of the 939 C domains detected in the 452 gene clusters
Maximum Likelihood phylogeny tree of all cyanobacteria included in the study
The Maximum-Likelihood phylogenetic species tree was generated using PhyML 3.1.0.2 with the alignment of a concatenation of twenty-nine conserved proteins
KS PUFA alignment dataset
The alignment used to generate the phylogeny of bacterial ketoacyl synthase domains
Maximum-likelihood phylogenetic tree of the 472 KS domains
Maximum-likelihood phylogenetic tree of the 472 KS domain