MINIMIZING HEAVY METAL IN CRAFT-SETTLEMENT WASTEWATER BY SULFATE-REDUCING BACTERIA-DESULFOVIBRIO DESSULFURICANS

Joint Research on Environmental Science and Technology for the Eart

DISTRIBUTION OF USEFUL AND HARMFUL MICROORGANISMS IN SHRIMP AQUACULTURE WATER IN TIEN HAI COASTAL OF THAI BINH PROVINCE

Joint Research on Environmental Science and Technology for the Eart

Replacing Face-To-Face Classes by Synchronous Online Technologies: The HOU Experience

AbstractSince 2009, HOU has been providing live virtual classes for various distance learningprograms.This paper will provide an opportunity to look at the issues involved in the use of thesemultimedia-enabled delivery approaches, the technology behind them, the logistics involved,and to provide an HOU perspective of the experiences encountered.The goal of research was to provide a systematic methods to implement the highlyinteractive live session. The additional goals was to design the portable hardware and easy touse software toolset as well as easy to follow guidelines on how to propel the lectures fromthe conventional dull chalk and talk and to minimise the number of staff required to give thelectures.Through a combination of surveys and feedback from lecturers and students, we are ableto better understand the obstacles and to continuously improve on the effectiveness of theseinteractive delivery approaches

DIVERSITY OF MICROORGANISMS IN SOME FAMOUS BAYS OF VIETNAM AND THEIR ABILITY TO USE IN AQUACULTURE

Joint Research on Environmental Science and Technology for the Eart

Crude oil-utilizing strain Desulfovibrio vulgaris D107G3, a mesophilic sulfate-reducing bacterium isolated from Bach Ho gas-oil field in Vung Tau, Vietnam

Some of anaerobic, mesophilic sulfate-reducing bacteria that produce H₂S and cause microbial metal corrosion can degrade crude oil in anaerobic conditions. In this study, a mesophilic sulfate-reducing bacterial strain D107G3 isolated from Bach Ho gas-oil field in Vung Tau, Vietnam that is able to utilize crude oil in the anaerobic condition is reported. The strain D107G3 was classified as a Gram-negative bacterium by using Gram staining method. Basing on scanning microscopy observation, the cell of a strain D107G3 had a curved rod shape. The 16S rRNA gene sequence analysis showed that the strain D107G3 was identified as Desulfovibrio vulgaris with 99.7% identity. The suitable conditions for its growth that was determined via estimating its H₂S production was the modified Postgate B medium containing 1% (v/v) crude oil, 1% NaCl (w/v), pH 7 and 300C incubation. In these conditions, the strain D107G3 can consume 11.4 % of crude oil total and oxidize heavy crude oil (≥ C45) for one month at anoxic condition. These obtained results not only contribute to the science but also continue to warn about the dangers of mesophilic sulfate reducing bacteria to the process of crude oil exploitation, use, and storage in Vung Tau, Vietnam.Trong bài báo này, chủng vi khuẩn khử sunphat (KSF) ưa ấm D107G3 phân lập từ giếng khoan dầu khí mỏ Bạch Hổ, Vũng Tàu, Việt Nam có khả năng sử dụng dầu thô trong điều kiện kị khí được công bố. Chủng D107G3 được xác định là vi khuẩn Gram âm nhờ phương pháp nhuộm Gram. Quan sát trên kính hiển vi điện tử quét cho thấy tế bào chủng D107G3 có hình que cong. Kết quả phân tích trình tự gen 16S rRNA đã xác định được chủng D107G3 thuộc loài Desulfovibrio vulgaris với độ tương đồng 99.7%. Thông qua đánh giá lượng H₂S tạo thành đã khám phá được điều kiện thích hợp cho sinh trưởng của chủng D107G3: môi trường Postgate B cải tiến chứa 1% (v/v) dầu thô, 1 % NaCl (gL⁻¹), pH 7 và nuôi cấy ở 30°C. Trong điều kiện đó, chủng D107G3 đã sử dụng được 11.4 % hàm lượng dầu tổng số, thành phần dầu bị phân huỷ là các n-parafin có mạch C≥45 sau 1 tháng nuôi cấy kỵ khí. Các kết quả này đóng góp về mặt khoa học và tiếp tục cảnh báo mối nguy hại của KSF ưa ấm đến việc khai thác, sử dụng và bảo quản dầu mỏ ở Vũng Tàu, Việt Nam

Differentiation Effect of Two Alkaloid Fractions from Vietnamese Lycopodiaceae on Mouse Neural Stem Cells

Various Lycopodium alkaloids have been studied for their various biological activities including anti-inflammatory, antioxidant, immunomodulatory, and neuroprotective activities. Moreover, these alkaloid compounds have high potential in the treatment of neuron degenerative disease. This study has been carried out to test the effect of Huperzia serrata (Thunb.) Trevis, and Lycopodium clavatum L alkaloid fractions on the mouse neural stem cells (NSCs). Firstly, the alkaloid fractions were used to verify its toxicity on NSCs. The multiple concentrations of alkaloid fractions from H. serrata (0.044; 0.088; 0.175; 0.35; 0.7; 1.4 mg/ml) and L. clavatum (0.031; 0.063; 0.125; 0.25; 0.50; 1.0; 2.0 mg/ml) have been used for the treatment of NSCs at period of 48h incubation. Results of the study suggested that the IC50 value of H. serrata and L. clavatum was 0.56 mg/ml and 0.50 mg/ml, respectively. Then, the NSCs were differentiated in the presence of 5 and 10 µg/ml of alkaloid fraction from H. serrata; 0.625 and 1.25 µg/ml of alkaloid fraction from L. clavatum for 6 days. Here, we observed the primary NSCs treated with alkaloid fraction extract from H. serrata showed the increased gene expression level of early neuron TUBB3 and neuron-specific cytoskeleton MAP2. On the other hand, the L. clavatum alkaloid fraction increased the expression of neural stem cell marker genes (Nestin and PAX6) and decreased neuron marker genes. In conclusion, these results established that alkaloid fraction from H. serrata promoted differentiation of the mouse NSCs to neuron cells, and L. clavatum extract had a capacity for stemness maintenance

The effect of some factors on expression of gene encoding endoglucanase from dna metagenome of Binh Chau hot spring in Escherichia coli

Expression of microbial target genes in Escherichia coli is broadly used due to its advantages namely: well established system, easy to manipulate, a huge biomass, high level productivity, safe and inexpensive to grow. Metagenomic technique has been applying in Vietnam recently for effective mining of uncultured gene resources, especially in endemic mini-ecologies such as hot springs where the cell densities are low. DNA metagenome of Binh Chau hot spring was isolated and sequenced by Illumia HiseqTM. Based on analyses of databases of cellulase-encoded genes, denovogenes 18736 gene sequence for thermal endoglucanase was selected for expression in E. coli. In this paper, some factors for expression of endoglucanase have been investigated. The results show that appropriate gene expression conditions are:  Expression performed in E. coli C43 (DE3) on TB medium at 30oC with 0.25 mM of IPTG as inducer, the culture volume of 20% compared with the bottle volume and the expression time is 42–48 hours. In this condition, the biomass production and soluble enzyme activity can reached up to 5.54–5.58 g /L and  1.92–1.98 U/mL, respectively. Our results show the prospect of exploiting microbial genes without culture

Student Satisfaction with Quality of Accounting and Auditing Education: The Case of Vietnam

This research is conducted to examine and evaluate determinants influencing the level of student satisfaction in the quality of auditing and accounting training at Vietnamese universities. Data were collected from 213 students of accounting and auditing field in three universities of National Economics University, University of Commerce and Academy of Finance. Exploratory factor analysis (EFA) and linear regression models were employed to determine the determinants influencing student satisfaction about quality of accounting and auditing. The findings show that the strongest impact on student satisfaction was the ability to serve; then training program; infrastructure component, and the last of teaching team. Based on the results, some recommendations are proposed for improving the satisfaction levels of students of accounting and auditing. Keywords: Quality of service; Quality of training; Satisfactio

Improving the efficiency of biodiversity investment - Final Report<br />

The overarching aim of this project was to provide information that would help improve the efficiency of biodiversity investments in northern Australia. We collected social and financial data from land managers across northern Australia, combined it with publically available biophysical data and analysed it using several different techniques. Controlling for a range of factors (e.g. size of property, rainfall), we found little evidence of a trade-off between biodiversity and agriculture, suggesting that conservation need not occur at the expense of agriculture in this region. We have also established that there are significant co-benefits (to agriculture) from programs that mitigate threats to biodiversity by controlling weeds. These programs represent more efficient investments than those which do not generate co-benefits. Our analysis also indicated that programs which promote on-farm diversification, improve land management practices and/or promote conservation-friendly attitudes could generate improvements in biodiversity without imposing costs on the agricultural industry, and that those who seek to promote biodiversity using financial rewards or penalties could increase the effectiveness of their programs by also using social rewards and penalties