Leukemia cell signaling and signaling of non-malignant B-cells

Leukemia cell signaling Acute leukemia (AL) is the most common pediatric cancer. Approximately 90 - 100 children is diagnosed every year in the Czech Republic. Acute leukemia is a complex disease that is pathologically manifested at the DNA, mRNA, protein and cellular level. Leukemic cells aberrantly express molecules that are found in other cell types under physiological conditions and their functional involvement in leukemic cells is unknow. We found that aberrantly expressed CEACAM6 increases the expression and affinity of integrins, increases the phosphorylation of intracellular kinases Akt, p38MAPK and p44/42 MAPK and triggers apoptosis in B- cell precursor acute lymphoblastic leukemia cells. Adaptor molecule NTAL, aberrantly expressed in T-cell acute lymphoblastic leukemia, signals through intracellular kinase p44/42 MAPK and potentiates corticosteroid induced apoptosis. Current leukemia research is focused mainly on monitoring of mutations at the DNA level, however, the functional consequences of these changes on cellular machineries are not straightforward. Since proteome analysis can provide link between gene sequence and cellular physiology, proteomics will contribute to elucidate mechanism of disease, prognosis and response to treatment. Protein microarrays technology is of major..

Phylogenetic analyses.

<p>Phylogenetic tree for the HA gene (AA based) as well as the results on the genetic analyses of the NA gene, antigenic typing results and information on the time of sample collection of each virus; viruses in colour and framed by red rectangles indicate reference viruses; viruses in bold and with * indicate vaccinated influenza positive cases; viruses in red and bold indicate influenza B viruses with HA and NA from distinct influenza B lineages. (4A) detailed results of 59 influenza A(H1N1)pdm09 viruses, (4B) detailed results of 98 A(H3N2) viruses, and (4C) detailed results of 58 influenza B viruses.</p

Participants Profile.

<p>Participants Profile.</p

Inclusion and exclusion criteria.

<p>Inclusion and exclusion criteria applied to the data set used for VE estimates.</p

Vaccine effectiveness estimates.

<p>Vaccine effectiveness estimates.</p

Regions of origin by place of birth of included patients.

<p>(A) Light gray: Western Europe; Dark gray: Eastern Europe. (B) Light gray: Middle East.</p

ROC curve for the multiple logistic regression model for CMV serostatus using a set of selected predictors.

<p>ROC curve for the multiple logistic regression model for CMV serostatus using a set of selected predictors.</p

Nomogram to predict maternal CMV seropositivity in early pregnancy.

<p>Points for each variable are read from the matching lower scale. The sum of the points plotted on the sum score line corresponds with the prediction of maternal CMV seropositivity, which is assigned by drawing a vertical line to the probability scale. Parental Migration Background (MB) is indicated in four classes: 00, both parents have no MB; 01, only the father has MB; 10, only the mother has MB; 11 both parents have MB. Education status indicates maternal Education Status as defined above.</p

The numbers of specimens with a positive RIDT result were determined within four intervals of cycle threshold (Ct) values: 20.3–27.1, 27.2–30.3, 30.5–34.8, 34.9–41.6 (quartiles of Ct values).

<p>The numbers of specimens with a positive RIDT result were determined within four intervals of cycle threshold (Ct) values: 20.3–27.1, 27.2–30.3, 30.5–34.8, 34.9–41.6 (quartiles of Ct values).</p

Performance of the QuickVue Influenza A+B rapid test in comparison with RT-PCR in the diagnosis of pandemic H1N1 (2009) virus infection.^a

a<p>confidence interval (CI).</p