7 research outputs found
Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action
Chemogenomic screens were performed in both budding and fission yeasts, allowing for a cross-species comparison of drugāgene interaction networks.Drugāmodule interactions were more conserved than individual drugāgene interactions.Combination of data from both species can improve drugāmodule predictions and helps identify a compound's mode of action
Gene expression profile of glioblastoma multiforme invasive phenotype points to new therapeutic targets
AbstractThe invasive phenotype of glioblastoma multiforme (GBM) is a hallmark of malignant process, yet molecular mechanisms that dictate this locally invasive behavior remain poorly understood. Gene expression profiles of human glioma cells were assessed from laser capture-microdissected GBM cells collected from paired patient tumor cores and white matterinvading cell populations. Changes in gene expression in invading GBM cells were validated by quantitative reverse transcription polymerase chain reaction (QRTPCR) and immunohistochemistry in an independent sample set. QRT-PCR confirmed the differential expression in 19 of 21 genes tested. Immunohistochemical analyses of autotaxin (ATX), ephrin 133, B-cell lymphoma-w (BCLW), and protein tyrosine kinase 2 beta showed them to be expressed in invasive glioma cells. The known GBM markers, insulin-like growth factor binding protein 2 and vimentin, were robustly expressed in the tumor core. A glioma invasion tissue microarray confirmed the expression of ATX and BCLW in invasive cells of tumors of various grades. GBM phenotypic and genotypic heterogeneity is well documented. In this study, we show an additional layer of complexity: transcriptional differences between cells of tumor core and invasive cells located in the brain parenchyma. Gene products supporting invasion may be novel targets for manipulation of brain tumor behavior with consequences on treatment outcome
Keeping It Together in Times of Stress: Checkpoint Function at Stalled Replication Forks
Gene Expression Profile of Glioblastoma Multiforme Invasive Phenotype Points to New Therapeutic Targets
Colocalization of Mec1 and Mrc1 is sufficient for Rad53 phosphorylation in vivo
In the DNA damage checkpoint, the sensor kinase Mec1 must be activated by Ddc1 or Dpb11. However, Ddc1 and Dpb11 are dispensable for the related replication checkpoint. Instead, colocalization of Mec1 and the replisome component Mrc1 is the minimal signal required to activate the replication checkpoint and allow survival of replication stress