48 research outputs found
EFFECT OF CE3+ METAL IONS ON THE ANTIBACTERIAL AND ANTICANCER ACTIVITY OF ZINC OXIDE NANOPARTICLES PREPARED BY COPRECIPITATION METHOD
ABSTRACTObjective: This study was undertaken to know about the antibacterial and anticancer activity of synthesized zinc oxide (ZnO) nanoparticles (NPs).Methods: The ZnO NPs and different concentration of Ce3+ (0.05M, 0.10M, and 0.15M)-doped ZnO NPs were synthesized by coprecipitation method.The synthesized nanoparticles were analyzed by X-ray diffraction (XRD) and HRSEM. The antibacterial studies were performed against a set ofbacterial strains as Gram-positive bacteria (Streptococcus aureus and Streptococcus pneumonia) and Gram-negative (Escherichia coli, Pseudomonasaeruginosa, Proteus vulgaris, Klebsiella pneumonia, and Shigella dysenteriae) bacteria. The cytotoxic effect of ZnO and Ce-doped ZnO was analyzed incultured (A549) human lung cancer cell line.Result: The XRD studies showed the wurtzite structure of nanoparticles. HRSEM analysis showed the spherical shape of ZnO and Ce-doped ZnO. TheZn0.85Ce0.15O NPs possessed more antibacterial effect as compared to the other ZnO and Ce-doped ZnO NPs. The Zn0.90Ce0.10O NPs created the highestcytotoxicity activity. With respect to cell death, as low a concentration of 68±0.05 μg/ml of Zn0.90Ce0.10O NPs was good enough to cause loss of viabilityof 50% of the cell as compared to ZnO and Zn1-xCexO (x=0.05 and 0.15) NPs.Conclusion: Results from this work concluded that Zn0.85Ce0.15O and Zn0.90Ce0.10O NPs possess antibacterial and anticancer activity, respectively.Keywords: Zinc oxide nanoparticles, Coprecipitation method, Antibacterial activity and anticancer activity, Human lung cancer cell line
Adsorptive Removal of Crystal Violet Dye Using Agricultural Waste Cocoa (theobroma cacao) Shell
Abstract In this study, the ability of Cocoa (Theobroma cacao) Shell Activated Carbon (CSAC
Preparation of porous bio-char and activated carbon from rice husk by leaching ash and chemical activation
Purification and characterization of membrane-bound <em>Borassus flabellifer</em> L. thermostable peroxidase
273-279Generating wealth out of waste is a goal in solid waste management. Borassus flabellifer shells with stone part are discarded as waste after removing its nutritious fruit. An enzyme extracted from the discarded stone part has potential application in molecular diagnosis and it is a wealth. The waste stone part bound Borassus flabellifer peroxidase was purified by salting-out, salting-in and DEAE-Cellulose anion exchange chromatographic technique to apparent homogeneity. Relative molecular weight under denaturating condition was around 40 kDa. The preparation had single isoenzyme as evidenced under nondenaturating condition. It was a glyco- and hemoprotein. It retained 100% activity for 120 h at 70°C. pH optima with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was around 5.0. Kinetic studies showed it had a higher affinity towards tetramethyl benzidine than other three substrates. The thermodynamic parameter of the enzyme with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was 35, 1.3, 35 × 106 KJ/mole and 1007 × 106 KJ/mole respectively. It obeyed Ping-Pong kinetics. The fluorescence intensities of the enzyme increased linearly as hydrogen peroxide concentration increased due to exposure of its hydrophobic moiety to the environment. Peel staining with Guaiacol substantiated it as a membrane-bound protein. Peroxidase was inhibited reversibly by various aromatic alcohols and its IC50 values were determined
Purification and characterization of membrane-bound <em>Borassus flabellifer</em> L. thermostable peroxidase
273-279Generating wealth out of waste is a goal in solid waste management. Borassus flabellifer shells with stone part are discarded as waste after removing its nutritious fruit. An enzyme extracted from the discarded stone part has potential application in molecular diagnosis and it is a wealth. The waste stone part bound Borassus flabellifer peroxidase was purified by salting-out, salting-in and DEAE-Cellulose anion exchange chromatographic technique to apparent homogeneity. Relative molecular weight under denaturating condition was around 40 kDa. The preparation had single isoenzyme as evidenced under nondenaturating condition. It was a glyco- and hemoprotein. It retained 100% activity for 120 h at 70°C. pH optima with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was around 5.0. Kinetic studies showed it had a higher affinity towards tetramethyl benzidine than other three substrates. The thermodynamic parameter of the enzyme with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was 35, 1.3, 35 × 106 KJ/mole and 1007 × 106 KJ/mole respectively. It obeyed Ping-Pong kinetics. The fluorescence intensities of the enzyme increased linearly as hydrogen peroxide concentration increased due to exposure of its hydrophobic moiety to the environment. Peel staining with Guaiacol substantiated it as a membrane-bound protein. Peroxidase was inhibited reversibly by various aromatic alcohols and its IC50 values were determined
Studies on cerium(III) complexes of piperidin-4-ones
1417-1420Ten new cerium
(III) nitrate complexes of the general formula
[Ce(L)2(NO3)2(H2O)2]NO3
with variously substituted 2,6-diphenylpiperidin-4-ones (L1)-(L10)
have been prepared in ethanol and characterized by elemental analysis, molecular
weights, molar conductance, magnetic and thermal studies and infrared and NMR spectra.
Molar conductance data and van't Hoff factor (i = 2) show that these complexes are
1: 1 electrolytes. The IR spectral data reveal the participation of water molecules
and thermal studies confirm the presence of two coordinated water molecules. IR
spectra, elemental analyses and molar conductance data suggest that these complexes
contain one ionic and two bidentate nitrate groups. Although piperidin-4-ones have
two coordinating
sites, namely
the ring nitrogen and carbonyl group, IR and NMR spectral evidences are in favour
of the ring nitrogen as the coordinating group and not the carbonyl group, thereby
indicating that these ligands are monodendate. All the observations lead to the
conclusion that cerium has a coordination number of eight in these complexes
Removal of Malachite Green from Aqueous Solution by Activated Carbon Developed from Cocoa (Theobroma Cacao) Shell - A Kinetic and Equilibrium Studies
The removal of malachite green (MG) by cocoa (Theobroma cacao) shell activated carbon (CSAC) was investigated in present study. Adsorption studies were performed by batch experiments as a function of process parameters such as initial pH, contact time, initial concentration and adsorbent dose. A comparison of kinetic models applied to the adsorption of MG on CSAC was evaluated for the pseudo-first order and pseudo-second order kinetic models. Results showed that the pseudo-second order kinetic model was found to correlate the experimental data well. The experimental equilibrium adsorption data was represented with Langmuir, Freundlich, Tempkin, Dubinin-Radushkevich and Flory-Huggins isotherms. The experimental data obtained in the present study indicated that activated carbon developed from cocoa shell can be attractive options for dye removal from waste water
Conformational Studies of Substituted Chromans: Crystal Structures of 2,2 '-(1E,1 ' E)-(2,3-Dimethylchroman-2,4-diyl)bis(Azan-1-yl-1-ylidene)bis(Methan-1-yl-1-ylidene)Diphenol (I) and 2,2 '-(1E,1 ' E)-(2-Ethyl-3-Methylchroman-2,4-diyl)bis(Azan-1-yl-1-ylidene)bis(Methan-1-yl-1-ylidene)Diphenol (II)
The title compounds C(25)H(24)N(2)O(3) (I) and C(26)H(26)N(2)O(3) (II) crystallize in the triclinic space group P-1 with cell parameters a = 8.981(1), b = 9.933(1), c = 12.369(2) , alpha = 78.537(2), beta = 84.515(2), gamma = 73.561(2)degrees Z = 2 (I) and a = 11.4630(9), b = 12.955(1), c = 16.154(1) , alpha = 70.425(1), beta = 87.403(1), gamma = 71.850(1)A degrees, Z = 4 (II). In both compounds the phenolic groups in the Schiff base substituents form intramolecular hydrogen bonds with the imine nitrogen atoms thereby rendering these substituents nearly planar. A detailed analysis of the amount by which the heterocyclic ring deviates from planarity (extent of puckering) in these and a series of related molecules shows that the extent of the deviation is largely unaffected by the number, size and placement of substituents on this ring