Host Transcription Profile in Nasal Epithelium and Whole Blood of Hospitalized Children Under 2 Years of Age With Respiratory Syncytial Virus Infection.

BACKGROUND: Most insights into the cascade of immune events after acute respiratory syncytial virus (RSV) infection have been obtained from animal experiments or in vitro models. METHODS: In this study, we investigated host gene expression profiles in nasopharyngeal (NP) swabs and whole blood samples during natural RSV and rhinovirus (hRV) infection (acute versus early recovery phase) in 83 hospitalized patients <2 years old with lower respiratory tract infections. RESULTS: Respiratory syncytial virus infection induced strong and persistent innate immune responses including interferon signaling and pathways related to chemokine/cytokine signaling in both compartments. Interferon-α/β, NOTCH1 signaling pathways and potential biomarkers HIST1H4E, IL7R, ISG15 in NP samples, or BCL6, HIST2H2AC, CCNA1 in blood are leading pathways and hub genes that were associated with both RSV load and severity. The observed RSV-induced gene expression patterns did not differ significantly in NP swab and blood specimens. In contrast, hRV infection did not as strongly induce expression of innate immunity pathways, and significant differences were observed between NP swab and blood specimens. CONCLUSIONS: We conclude that RSV induced strong and persistent innate immune responses and that RSV severity may be related to development of T follicular helper cells and antiviral inflammatory sequelae derived from high activation of BCL6

A method to improve protein subcellular localization prediction by integrating various biological data sources

<p>Abstract</p> <p>Background</p> <p>Protein subcellular localization is crucial information to elucidate protein functions. Owing to the need for large-scale genome analysis, computational method for efficiently predicting protein subcellular localization is highly required. Although many previous works have been done for this task, the problem is still challenging due to several reasons: the number of subcellular locations in practice is large; distribution of protein in locations is imbalanced, that is the number of protein in each location remarkably different; and there are many proteins located in multiple locations. Thus it is necessary to explore new features and appropriate classification methods to improve the prediction performance.</p> <p>Results</p> <p>In this paper we propose a new predicting method which combines two key ideas: 1) Information of neighbour proteins in a probabilistic gene network is integrated to enrich the prediction features. 2) Fuzzy k-NN, a classification method based on fuzzy set theory is applied to predict protein locating in multiple sites. Experiment was conducted on a dataset consisting of 22 locations from Budding yeast proteins and significant improvement was observed.</p> <p>Conclusion</p> <p>Our results suggest that the neighbourhood information from functional gene networks is predictive to subcellular localization. The proposed method thus can be integrated and complementary to other available prediction methods.</p

Spatiotemporal evolution of SARS-CoV-2 Alpha and Delta variants during large nationwide outbreak of COVID-19, Vietnam, 2021

We analyzed 1,303 SARS-CoV-2 whole-genome sequences from Vietnam, and found the Alpha and Delta variants were responsible for a large nationwide outbreak of COVID-19 in 2021. The Delta variant was confined to the AY.57 lineage and caused >1.7 million infections and >32,000 deaths. Viral transmission was strongly affected by nonpharmaceutical interventions

EFETIVIDADE DA INTERVENÇÃO EDUCACIONAL DIGICARE NA MELHORIA DAS HABILIDADES DE COACHING CLÍNICO DE ESTUDANTES DE ENFERMAGEM E MEDICINA NO VIETNAME E BANGLADESH: UM PRÉ- E PÓS-ESTUDO EXPLORATÓRIO

Coaching has become an important approach to support self-management of patients with non-communicable diseases (NCDs) in healthcare education. Studies conducted in European countries have emphasized the significance of formal coaching training in enhancing the competencies of healthcare students. However, in Southeast Asia, where NCDs pose a serious public health concern, there is a lack of such training opportunities. To address this issue, an exploratory pre and post study was conducted to evaluate the effectiveness of the DigiCare educational intervention in improving clinical coaching skills. Nursing and medical students from six universities in Vietnam and Bangladesh were invited to participate. The intervention included both theoretical and practical classes with interactive methods and home assignments, with a total duration of over 10 contact hours. Pre- and post-intervention assessments were conducted using the Self-Efficacy and Performance in Self-management Support instrument, which was translated and culturally adapted to both countries. Statistical analysis showed a significant improvement in students’ overall competence scores from before (M = 2.6, SD = .67) to after the intervention (M = 3.05, SD = .55), with a medium effect size (p &lt; .001; d = .73). The DigiCare educational intervention appears to be a low-cost and meaningful addition to the curriculum of both nursing and medical universities across countries, with potential benefits in the development of students’ clinical coaching competencies.O coaching tornou-se uma abordagem importante para apoiar a autogestão de pacientes com doenças não transmissíveis (DNTs) na educação em saúde. Estudos realizados em países europeus têm enfatizado a importância do treinamento formal em coaching para aprimorar as competências dos estudantes de saúde. No entanto, no Sudeste Asiático, onde as DNTs representam uma séria preocupação de saúde pública, há uma falta de oportunidades de treinamento nesse sentido. Para abordar essa questão, foi conduzido um pré- e pós-estudo exploratório para avaliar a eficácia da intervenção educacional DigiCare na melhoria das habilidades de coaching clínico. Estudantes de enfermagem e medicina de seis universidades no Vietname e em Bangladesh foram convidados a participar. A intervenção incluiu aulas teóricas e práticas com métodos interativos e tarefas domiciliares, totalizando mais de 10 horas de contato. Avaliações pré e pós-intervenção foram conduzidas utilizando o instrumento de Autoeficácia e Desempenho no Suporte à Autogestão, que foi traduzido e adaptado culturalmente para ambos os países. Análises estatísticas mostraram uma melhoria significativa nas pontuações gerais de competência dos estudantes, de antes (M = 2,6, DP = 0,67) para depois da intervenção (M = 3,05, DP = 0,55), com um efeito médio (p &lt; 0,001; d = 0,73). A intervenção educacional DigiCare parece ser uma adição de baixo custo e significativa para o currículo de universidades de enfermagem e medicina em diferentes países, com benefícios potenciais no desenvolvimento das competências clínicas de coaching dos estudantes

Republic of Korea

doi:10.1093/bioinformatics/btq44

Assessment of the Role of Ginsenoside RB1 Active Substance in Alginate/Chitosan/Lovastatin Composite Films

This article reports the effect of ginsenoside Rb1 on some properties, morphology, and the drug release process of the chitosan (CS)/alginate (AG)/lovastatin (LOV) composite films prepared by a solution method using different contents of ginsenoside Rb1. The ratio of AG/CS was fixed at 4/1 (wt.%/wt.%), the content of LOV was 10 wt.%, and the content of ginsenoside Rb1 was changed from 1 to 5 wt.%. The results of scanning electron microscopy and Fourier transform infrared spectroscopy analysis showed that the composite films have a heterogeneous structure and the ginsenoside Rb1 content influenced on the structure of composite films. The presence of ginsenoside Rb1 did not influence on the melting temperature of these films but caused a significant difference in the melting enthalpy of the films. The ginsenoside Rb1 was also contributed positively on the LOV release from these films in different pH buffer solutions. The LOV release process from these films included two stages (fast/burst release and slow/control release). It was increased remarkably by the synergic effect of LOV and ginsenoside Rb1 in the drug release process. From the obtained results, we suggested that ginsenoside Rb1 plays an important role not only in the enhancement of health and immunity as general but also as an efficient agent in control of the LOV size as well as LOV drug release from the composite films

The Engineering of Porous Silica and Hollow Silica Nanoparticles to Enhance Drug-loading Capacity

As a promising candidate for expanding the capacity of drug loading in silica nanoplatforms, hollow mesoporous silica nanoparticles (HMSNs) are gaining increasing attention. In this study, porous nanosilica (PNS) and HMSNs were prepared by the sol-gel method and template assisted method, then further used for Rhodamine (RhB) loading. To characterize the as-synthesized nanocarriers, a number of techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), nitrogen absorption-desorption isotherms, dynamic light scattering (DLS), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR) were employed. The size of HMSN nanoparticles in aqueous solution averaged 134.0 &plusmn; 0.3 nm, which could be adjusted by minor changes during synthesis, whereas that of PNS nanoparticles was 63.4 &plusmn; 0.6 nm. In addition, the encapsulation of RhB into HMSN nanoparticles to form RhB-loaded nanocarriers (RhB/HMSN) was successful, achieving high loading efficiency (51.67% &plusmn; 0.11%). This was significantly higher than that of RhB-loaded PNS (RhB/PNS) (12.24% &plusmn; 0.24%). Similarly, RhB/HMSN also possessed a higher RhB loading content (10.44% &plusmn; 0.02%) compared to RhB/PNS (2.90% &plusmn; 0.05%). From those results, it is suggested that prepared HMSN nanocarriers may act as high-capacity carriers in drug delivery applications