The roles of specific glycosylases in determining the mutagenic consequences of clustered DNA base damage

The potential for genetic change arising from specific single types of DNA lesion has been thoroughly explored, but much less is known about the mutagenic effects of DNA lesions present in clustered damage sites. Localized clustering of damage is a hallmark of certain DNA-damaging agents, particularly ionizing radiation. We have investigated the potential of a non-mutagenic DNA base lesion, 5,6-dihydrothymine (DHT), to influence the mutagenicity of 8-oxo-7,8-dihydroguanine (8-oxoG) when the two lesions are closely opposed. Using a bacterial plasmid-based assay we present the first report of a significantly higher mutation frequency for the clustered DHT and 8-oxoG lesions than for single 8-oxoG in wild-type and in glycosylase-deficient strains. We propose that endonuclease III has an important role in the initial stages of processing DHT/8-oxoG clusters, removing DHT to give an intermediate with an abasic site or single-strand break opposing 8-oxoG. We suggest that this mutagenic intermediate is common to several different combinations of base lesions forming clustered DNA damage sites. The MutY glycosylase, acting post-replication, is most important for reducing mutation formation. Recovered plasmids commonly gave rise to both wild-type and mutant progeny, suggesting that there is differential replication of the two DNA strands carrying specific forms of base damage

Modeling of activated carbon and coal gasification char absorbents in single-solute and bisolute systems

A mathematical model of fixed-bed adsorption was used to predict the bed response to a sustained step change in influent concentration. The model was employed to compare the performance of different adsorbents in the removal of organics from water and to analyze factors that affect desorption owing to a decrease in influent concentration and to competitive adsorption. Model equations, which considered that film transfer and surface diffusion controlled the adsorption rate, were solved with the technique of orthogonal collocation. Three species, 3,5-dimethylphenol (DMP), 3,5-dichlorophenol (DCP) and rhodamine 6G (R6G), were the single solutes studied, and the two phenols were also examined as a mixture. Four activated carbons and a coal gasification char were the adsorbents studied. The model was used to compare the adsorbents in the removal of DMP, R6G and the bisolute mixtures and equilibrium capacity was found to have a greater influence than kinetics on fixed-bed performance. It was observed that, under conditions approximating a drinking water plant, the time during which the effluent concentration of a desorbed species was higher than the influent concentration was significant (on the order of weeks) whether a reduced influent concentration or competition was responsible for the desorption.U.S. Department of the InteriorU.S. Geological SurveyOpe

Multiphoton localization and propagating quantum gap solitons in a frequency gap medium

The many-particle spectrum of an isotropic frequency gap medium doped with impurity resonance atoms is studied using the Bethe ansatz technique. The spectrum is shown to contain pairs of quantum correlated ``gap excitations'' and their heavy bound complexes (``gap solitons''), enabling the propagation of quantum information within the classically forbidden gap. In addition, multiparticle localization of the radiation and the medium polarization occurs when such a gap soliton is pinned to the impurity atom.Comment: 8 pages, RevTEX, to appear in Phys. Rev. Let

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http://archive.org/details/fieldvisibilityc00thacNAN

Dehydration and Mortality of Feral Horses and Burros: a Systematic Review of Reported Deaths

Water is a requirement for all organisms, including equids. Dehydration-caused mortality of feral horses (Equus ferus caballus) is often cited as a cause of concern and as justification for management of feral horses, yet a paucity of information exists on the matter. We conducted a systematic review from September 1, 2020 through January 15, 2021 of available news reports of feral horse and burro (E. asinus) dehydration mortalities and public interventions to save horses using a public search engine with a priori defined search term combinations and additional snowball sampling. We found 15 uniquely reported mortality incidents representing 744 horse in the United States and Australia that occurred between 1976 and 2019; no similar reports for burros were found. Mortalities occurred during hotter and drier than normal conditions with occurrences escalating through the summer and fall. The number of horses per dehydration mortality event ranged from 1–191 with a mean of 50 horses. Mortalities occurred on a wide range of land jurisdictions including private lands, tribal lands, national forests, national parks, and Bureau of Land Management lands. Increasing feral horse populations in western North America and Australia, coupled with the drought forecasts over the next century, simply cannot be ignored. This study represents the first global and longitudinal assessment of feral horse dehydration mortalities

Estimation of the specificity of an antibody ELISA for paratuberculosis generated from a sector of the UK cattle population using results from a paratuberculosis control programme

In the United Kingdom (UK) a voluntary programme to control paratuberculosis in cattle based on herd management and serological screening has been operating since 1998. The programme assigns a risk level to each participating herd according to the within herd seroprevalence and the confirmation of the presence of infection with Mycobacterium avium subspecies paratuberculosis (MAP) by faecal culture or polymerase chain reaction (PCR). From the outset a general concern over the specificity of the paratuberculosis antibody enzyme-linked immunosorbent assay (ELISA) resulted in the use of a faecal screen for the causal organism to negate or confirm infection in individual seropositive animals. Progress in improving the diagnostic tests has been gradual throughout the life of the programme and the under-pinning approach to using tests to determine the risk of paratuberculosis for a herd required to be re-examined. This study used a large data set of more than 143,000 test results over five years from the lowest paratuberculosis risk level category of herds to estimate the specificity of a commercially available paratuberculosis antibody ELISA for cattle. In each year of the study the estimated specificity reached or exceeded 0.998. We also examined the apparent impact that annual or more frequent application of the single intradermal comparative cervical tuberculin (SICCT) test for tuberculosis (TB), using purified protein derivatives of Mycobacterium bovis and Mycobacterium avium subspecies avium, had on specificity of the antibody ELISA for paratuberculosis. We found a statistically significant difference in three of the five years with herds that were officially tuberculosis free and not subject to frequent SICCT testing. This difference was small and considered to be of little practical importance for the paratuberculosis assurance programme. We concluded that, in the UK the mandatory TB surveillance programme of cattle herds is not a limiting factor in the use of serological testing to support herd-level assurance schemes for paratuberculosis. Furthermore, in paratuberculosis, where shedding of MAP is intermittent and the sensitivity of the commercially available PCR tests for detection MAP is highly variable, faecal screening of seropositive animals is an unreliable method for negating infection in seropositive cattle

Making waves in education

Making Waves in Education is a book of a collaborative nature, being a collection of chapters written by undergraduates studying B.A. Hons in Education at the Universities of Plymouth and York. Thirteen chapters, each from a different student, cover topics from learning theories to sex education, home education and autism. The chapters are well-organised and written, and they cover key topics in an accessible and thoughtful way. The chapters are generally well - referenced and present critical and balanced arguments. Many use hard statistics in an effective way to back up their points and all include bibliographies as indeed one expects from a serious publication. The collection therefore addresses itself to a wide readership of anyone interested in education, and students and teachers/trainers in HE in particula

Coal Resources of the Upper Elkhorn No. 3A Coal (Lower Bed) in Eastern Kentucky

This map is one of a series that shows the regional characteristics of the Upper Elkhorn No. 3 coal zone. The maps were prepared as part of the U.S. Geological Survey\u27s National Coal Assessment Program, which compiles regional maps and databases that provide a comprehensive assessment of the most important coal beds in the nation. The Upper Elkhorn No. 3 coal zone has been one of the leading producers in the state of Kentucky and, in some areas, is of very high quality. Bed stratigraphy within the Upper Elkhorn No. 3 zone and coal thickness of the No. 3A coal are described in KGS Map and Chart Series 7 (series 12). The map shows mined-out areas and thickness characteristics of remaining coal. Figure 1 shows point-data locations, and Figure 2 shows tonnage calculations for the Upper Elkhorn No. 3A coal (also known as the Kellioka or B seam south of the Pine Mountain Fault)

Coal Resources of the Upper Elkhorn No. 3B Coal (Upper Bed) in Eastern Kentucky

This chart is one of a series that shows the regional characteristics of the Upper Elkhorn No. 3 coal zone. The maps and charts were prepared as part of the U.S. Geological Survey\u27s National Coal Assessment Program, which compiles regional maps and databases that provide a comprehensive assessment of the most important coal beds in the nation. The Upper Elkhorn No. 3 coal zone has been one of the leading producers in the state of Kentucky and, in some areas, is of very high quality. Bed stratigraphy within the Upper Elkhorn No. 3 zone and thickness of the No. 3A coal are described in KGS Map and Chart Series 7-8 (series 12). This chart shows original total coal thickness (Fig. 1), mined-out areas (Fig. 2), mining facies (Fig. 3), and resource calculations (Fig. 4) for the Upper Elkhorn No. 3B coal (also know as the Darby or C seam south of the Pine Mountain Overthrust Fault). The coal-thickness map is not a traditional isopach map, because the mineable bed is not composed of the same benches in all areas (Fig. 3). Discontinuities, delineated by facies boundaries on the map, indicate abrupt changes in thickness caused by splitting; discontinuities also occur between areas where entirely different beds in the Upper Elkhorn No. 3 zone are favored for mining

Coal Resources of the Lower Elkhorn Coal Bed in Eastern Kentucky

This chart is one of a series that shows the regional characteristics of the Lower Elkhorn coal. The maps were prepared as part of the U.S. Geological Survey\u27s Natural Coal Assessment Program, which compiles regional maps and databases that provide a comprehensive assessment of the most important coal beds in the nation. The Lower Elkhorn coal is one of the leading producers in the state of Kentucky and has, in some areas, a reputation as an excellent metallurgical-grade coal. It is known locally as the Pond Creek, Imboden, Path Fork, Blue Gem, Straight Creek, Bruin or Vires coal bed. This chart describes the distribution of data used for the coal assessment, generalized mined-out areas in relation to coal thickness, geologic structure of the bed, and coal-resource estimates