Glyphosate impacts on polyphenolic composition in grapevine (Vitis vinifera L.) berries and wine

Glyphosate is the most widespread herbicide for weed management, being extensively used in viticulture. In this study we tested, under field conditions, the effects of glyphosate applications on the quality of berry and wine, from cv. Ancellotta (Vitis vinifera L.), with particular regard to anthocyanin concentration and composition. Ripening and growth were monitored by analyzing berry technological parameters and weight. Additionally, microvinifications were performed, in order to analyze the concentration of anthocyanins, other flavonoids and phenolic acids in wine. Our findings indicated that, at harvest, both pH and anthocyanin concentration were significantly lower and titratable acidity higher in berries collected from vines of plots under glyphosate-treatment compared with those of non-treated parcels. Data suggest that treatment with glyphosate did not change the concentration of anthocyanins, other flavonoids and phenolic acids in the wine. Our results indicate that treatment with glyphosate may affect fruit metabolism and nutritional value in non-target plants

The Nonmydriatic Fundus Camera in Diabetic Retinopathy Screening: A Cost-Effective Study with Evaluation for Future Large-Scale Application

Aims. The study aimed to present the experience of a screening programme for early detection of diabetic retinopathy (DR) using a nonmydriatic fundus camera, evaluating the feasibility in terms of validity, resources absorption, and future advantages of a potential application, in an Italian local health authority. Methods. Diabetic patients living in the town of Ponzano, Veneto Region (Northern Italy), were invited to be enrolled in the screening programme. The “no prevention strategy” with the inclusion of the estimation of blindness related costs was compared with screening costs in order to evaluate a future extensive and feasible implementation of the procedure, through a budget impact approach. Results. Out of 498 diabetic patients eligible, 80% was enrolled in the screening programme. 115 patients (34%) were referred to an ophthalmologist and 9 cases required prompt treatment for either proliferative DR or macular edema. Based on the pilot data, it emerged that an extensive use of the investigated screening programme, within the Greater Treviso area, could prevent 6 cases of blindness every year, resulting in a saving of €271,543.32 (−13.71%). Conclusions. Fundus images obtained with a nonmydriatic fundus camera could be considered an effective, cost-sparing, and feasible screening tool for the early detection of DR, preventing blindness as a result of diabetes

New oral anti-coagulants versus vitamin K antagonists in high thromboembolic risk patients.

BackgroundOral anticoagulant therapy (VKA) is nowadays the mainstay of treatment in primary and secondary stroke prevention in patients with atrial fibrillation. Given the limited risk-benefit ratio of vitamin K antagonists, pharmacological research has been directed towards the development of products that could overcome these limits, new oral anticoagulants were recently introduced: dabigatran, rivaroxaban, apixaban, and edoxaban.AimScope of the present study was to examine patterns of use, effectiveness, safety and mean annual cost per patient of anticoagulant treatment for non-valvular AF in real clinical practice.MethodsA retrospective observational cohort study, by using administrative databases (drugs, hospitalizations, clinical visits, lab tests, population registry), was conducted in the Local Health Unit (LHU) of Treviso, Italy, from January 1, 2012 to December 31, 2016.Results5597 subjects were selected, 2171 of which satisfied all inclusion criteria. In particular 1355 patients were treated with VKA, 577 patients were treated with NOAC, and 239 patients were treated initially with VKA and subsequently switched to NOAC (switch group). NOAC treatment showed to be superior to VKA and this superiority was statistically significant on both end-points: patients in the NOAC group reported less cardiovascular events (9,9%) and less bleeding episodes (5,5%) versus VKA patients (14,6% and 11,4%; pConclusionIn this retrospective real-world study treatment with NOAC showed to be associated with significant reductions of CV events and bleeding events compared to VKA use, albeit at a higher NHS' direct cost per patient/year, mainly due to higher drug therapy cost

Silicio, alleato in vigneto per migliorare la qualit\ue0 dell'uva

L\u2019USO di diversi composti contenenti silicio ha limitato i danni di alcuni problemi che insorgono nel corso della maturazione in particolare per temperature elevate e scarsa disponibilit\ue0 idrica. Non trattandosi di soluzioni risolutive, tuttavia, devono essere sommate a un\u2019accurata gestione della chioma e del suolo, a una buona disponibilit\ue0 di nutrienti nel vigneto e a un\u2019opportuna scelta del livello di esposizione della fascia produttiva. In particolare il caolino risulta ef\ufb01 cace in annate estreme, come nel 2012, riducendo il livello di decolorazione dei grappoli, le anomalie di maturazione, i fenomeni di scottatura da sole e di disidratazione. I prodotti a base di silicio non hanno alterato le caratteristiche organolettiche e sensoriali delle uve alla raccolta

CARD11 dominant negative mutation leads to altered human Natural Killer cell homeostasis

: Dominant negative mutations in CARD11 have been reported in patients with immune dysregulation, severe atopic features, and variable T cell alterations. Data on Natural killer (NK) cells from affected patients are lacking. We report on a 12-year-old boy with severe atopic dermatitis, food induced anaphylaxis and hypogammaglobulinemia harbouring a novel de novo heterozygous variant c.169G > A; p.Glu57Lys in CARD11. The dominant negative effect of this mutation was confirmed on both CD4+ and CD8+. CTLA4+Foxp3+CD4+ Tregs were severely reduced. Patient's NK cells showed reduced expression of NKp46, NKG2D and CD69. Patient's CD56bright NK cells showed in vitro impaired production of IFN-γ. Steady state pS6 levels on patient's NK cells were increased and remained elevated upon IL2 + IL12 + IL18 overnight stimulation. Overall, the effect of CARD11 mutation on mTORC1 differs between T and NK cells. These findings may explain the increased susceptibility to viral infections and the reduced immune surveillance in affected patients

Blood Meal-Based Compound. Good Choice as Iron Fertilizer for Organic Farming

Prevention of iron chlorosis with Fe synthetic chelates is a widespread agronomical practice but implies high costs and environmental risks. Blood meal is one of the main fertilizers allowed to be used in organic farming. Through this work a novel blood meal fertilizer was audited. Measurements such as FTIR, Raman, electron paramagnetic resonance, and M\uf6ssbauer spectroscopy, UV\u2013visible properties, stability against pH, and batch experiments were performed to characterize and assess the reactivity on soil constituents and agronomic soils. The spectroscopy findings give clear indications that Fe is in the ferric oxidation state, is hexacoordinated, and has a low-spin form suggesting a similar structure to hemin and hematin. A spectrophotometric method at 400 nm was validated to quantify blood meal concentration at low electrolyte concentrations. Batch experiments demonstrated high reactivity of blood meal fertilizer with soil constituents, mainly in the presence of calcium, where aggregation processes are predominant, and its ability to take Fe from synthetic Fe (hydr)oxides. The beneficial profile of blood meal by a providing nitrogen source together with the capability to keep the Fe bound to porphyrin organic compounds makes it a good candidate to be used as Fe fertilizer in organic farming

Enteral Nutrition in Nursing Home Residents: A 5-Year (2001-2005) Epidemiological Analysis

Background. Despite controversy and increasing use of enteral nutrition (EN) among elderly people, descriptive population-based data are scarce. The aim of this study was to evaluate the epidemiological data of nursing home residents (NHRs) who received EN in a northeast area of Italy, Methods. All NHRs referred to Our Nutrition Service for EN between 200 1 and 2005 were enrolled. Data collected at EN initiation included age, gender, underlying disease, Karnofsky index, type of enteral access device, presence of pressure ulcers, weight, body mass index, and daily enteral intake. The outcomes considered were patient survival and duration of therapy. Results. The 482 NHRs (130 males; 352 females) received EN. The mean incidence (cases/million population/year) and prevalence (cases/million population) were 223.4 and 279.4, respectively, An average of 6.6% of all NHRs were tube fed. EN was prescribed for the following conditions: 27.7% cerebrovascular accident, 54.6% neurodegenerative disease, 2.7% head and neck cancer, 1.2% abdominal cancer, 1.3% head trauma, 4.8% congenital disease, 7.7% other. Almost all patients had a Karnofsky index <= 50; 42.3% of patients had pressure ulcers. The median duration of EN was 296 days and the median overall Survival was 411 days. Conclusions. NHRs receiving EN were mainly afflicted with neurodegenerative and cerebrovascular diseases, functional impairments, and a high incidence of pressure sores. The mortality rate was low compared with others reported in literature. The low EN use among NHRs in our Study may Suggest a limited use in advanced dementia and at end-stage of life. (Nutr Clin Pract. 2009;24:635-641