Effect of bacterization with Aeromonas media GS4 and Pseudomonas extremorientalis PhS1 on wheat seedlings under different abiotic conditions

We studied the effect of soft wheat seed treatment (Triticum aestivum L.) with two bacterial strains (Aeromonas media GS4 and Pseudomonas extremorientalis PhS1) isolated from earthworm coprolites on the growth and development of wheat seedlings in a 12-day laboratory experiment, as well as on root rot disease and the activity of guaiacol-dependant peroxidase under optimal conditions and abiotic stress (elevated and low temperatures and moisture content). We established that growing nonbacterized wheat plants under stress abiotic conditions reduced the height of plants compared to growing under optimal abiotic conditions, and seed bacterization with P. extremorientalis PhS1 strain increased wheat plant height (by 9-15%) under stress abiotic conditions compared to the nonbacterized plants. Bacterization with both strains decreased infestation of wheat seedlings (2.5-4 times) by root rots under unfavorable abiotic conditions compared to nonbacterized plants. In addition, under optimal and arid conditions, bacterization with P. extremorientalis PhS1 strain was the most effective, and under humid conditions it was bacterization with A. media GS4 strain. We showed that the activity of guaiacol-dependant peroxidase correlates with the development of plant resistance to abiotic stress. In our experiments, plant bacterization resulted in a 2-fold increase in peroxidase activity both in leaves and roots of wheat plants compared to the nonbacterized plants. As the result, the ability of bacteria to activate peroxidase can serve as an information indicator of strengthening protective mechanisms of plants during bacterization

Prospects for the peat using as the basis of the soil-like substrate in mini-ecosystems modelling

Global urbanization is causing a constant decline in arable land as cities and associated industrial zones are "attacking" adjacent agricultural areas. One of the promising ways to solve the problem of increasing food production for the constantly growing population of the planet against the background of rapidly decreasing land resources is the development of fundamentally new alternative methods for the production of crop products, including in greenhouses. The fundamental basis for technological optimization of plant cultivation parameters and the output of the productive process of a particular crop to the maximum of its genetic capacities can be the development of artificial mini-ecosystems based on the reproduction of nature-like processes, implying the balance and combination in one volume of the processes of plant production and reduction of organic waste, initiated directly in the zone of the rhizosphere of plants due to the introduction of technological earthworms into the reduction zone. According to the results of model studies presented in this article, peat is an acceptable basis for the substrate of the root block of a mini-ecosystem, and the introduction of earthworms Eisenia fetida Sav. into the reduction zone does not have a negative effect on lettuce plants, provided that it is used as an energy substrate for cattle manure worms in quantities not exceeding 10 - 20% of the total volume of the substrate

Pseudomonads associated with soil lumbricides as promising agents in root rot biocontrol for spring grain crops

Currently, crop yields can be increased by high farming standards which include environmentally friendly use of chemical fertilizers and pesticides, as well as their replacement by bioformulations having similar activity. That is why both search for new promising species, strains and isolates of bacterial antagonists for their potential use as biocontrol agents, and study of antifungal activity mechanisms, particularly the relationship between the activity in model tests and in agrocenoses, are relevant. The aim of this study was to estimate bacterial isolates from redworm coprolites as potential bioactive agents to control phytopathogenic fungi causing root rot of crops. The experiments were conducted in 2013-2015. In the preliminary laboratory screening for fungistatic and growth-promoting activity we selected two strains, Pseudomonas sp. GS4 and Pseudomonas sp. PhS1, and assessed their ability to decrease the growth rate of fungal colonies in Petri dish test on nutrient agar medium and to reduce seed infestation of soft wheat (Triticum aestivum L., Irgin cultivar) in sterile paper roll test. Seeds soaked in distilled water served as control. As a standard, we used seed treatment with a chemical fungicide Dividend® Star («Syngenta AG», Switzerland) (30 g/l difenoconazole, 6.3 g/l cyproconazole) at recommended rates. In field tests, we recorded root rots in soft wheat Irgin cultivar plants and in barley (Hordeum vulgare L.) Acha cultivar plants during tillering and beginning of blooming. The laboratory tests showed a statistically significant (р < 0.05) 1.5-2.5-fold decrease in the growth rate of phytopathogenic fungi Fusarium oxysporum, Bipolaris sorokiniana and Alternaria spp. as compared to control. In all experiments with bacterization, there was a 53-76 % decrease (р < 0.05) in total seed infestation by pathogens as compared to non-bacterized plants. The effect of the bacteria in planta was assessed in small model systems. The obtained data show a statistically significant (р < 0.05) reduction in the root rot disease incidence in bacterization with Pseudomonassp. GS4 (by 33-37 %) and Pseudomonas sp. PhS1 (by 57-60 %). Root rot disease severity decreases 2.1-2.4-fold and 3.3-3.5-fold, respectively. In 2015, we revealed a tendency towards a 19-70 % increase in the total number of rhizosphere microorganisms at the beginning of plant blooming depending on the crop and type of bacterization. The number of hosphate-mobilizing bacteria in the rhizosphere under bacterization was, on average, 5.5-7.2-fold higher in wheat and 2.1-3.2-fold higher in barley than that without bacterization. Our results of root rot field study in the 2013-2015 showed the efficacy of both monocultures and complex bacterization which provided a decrease in wheat and barley root rot disease severity by 6.5-57.6 % and 18.6-50.0 %, respectively, depending on the bacterial culture and the weather conditions. The maximum biological efficacy of the isolates is noted at the beginning of blooming

Terms and Need Strategic Cooperation Mechanisms of Social and Economic Systems of the Region

The increasing complexity of the socio-economic systems and the increase in the state's functions in modern regional space becoming more common complex, multi-level strategic cooperation mechanisms. In such structures, the main elements of the control system are divided into several levels. Management levels are organizationally separate elements detected in the territorial structures of governance, for example, the subject of the Federation, municipal formation. Between the different levels of the system of separation of powers, responsibilities and functions of the power vertical. Within the framework of the powers assigned to them elements of the system have the right to independent decision-making. Developing of the Russian Federation system of market relations qualitatively increased value, role, authority and responsibility of regions to ensure the functioning and development of the territories, the growth of living standards of the population living in them. Enhanced functions of the organs of power in the federal subjects is accompanied by the simultaneous complexity of the mechanism of management decision-making, and the increasing influence of the variability of environmental factors. Under these circumstances, the effective activities of the regional administrations are not possible without the coordination and integration of their efforts, which can promote inter-regional cooperation. Keywords: regional economy, social and economic system, management mechanism JEL Classifications: Q27, R12, R5

Utilizing heavy metal-laden water hyacinth biomass in vermicomposting

We studied the efficiency of water treatment by water hyacinth (Eichhornia crassipes) from heavy metals (Zn

Utilizing heavy metal-laden water hyacinth biomass in vermicomposting

We studied the efficiency of water treatment by water hyacinth (Eichhornia crassipes) from heavy metals (Zn

Effect of Pseudomonas bacteria on peroxidase activity in wheat plants when infected with Bipolaris sorokiniana

We investigated the effect of treating soft wheat seeds (Triticum aestivum L.) with two Pseudomonas bacteria strains, isolated from earthworm coprolites, showing a significant antifungal and growth-promoting action in preliminary screening on the activity of guaiacol-dependant peroxidase under phytopathogenic load in the presence of Bipolaris sorokiniana (Sacc.) Shoemaker as a mechanism for inducing plant resistance to the pathogen. We established a statistically significant decrease (P < 0.05) in root rot disease incidence and severity during bacterization, which is indicative both of antifungal activity of the used bacterial isolates and of their successful colonizing the rhizosphere of wheat plants. We noted a response of free and weakly bound peroxidase of wheat plants to infection with B. sorokiniana: the enzyme activity increased during pathogenesis. Bacterization also increased peroxidase activity in plant leaves and roots, the greatest differences from non-bacterized plants being observed in wheat roots in the presence of the pathogen. We detected a direct link between peroxidase activity in wheat roots and leaf tissues in the absence of the pathogen and the feedback between peroxidase activity and plant infestation by the root rot pathogen. In the presence of the phytopathogen, there is a lack of correlation between peroxidase activity in wheat roots and leaves, and there is a shift of activity towards its increase in roots, which plays an important role in the development of systemic resistance against the root rot pathogen that penetrates into plants through the roots and root collar