La inquietud de Erika

Duración (en horas): De 31 a 40 horas. Destinatario: Estudiante y Docente“La inquietud de Erika” presenta una situación donde los estudiantes de odontología han de analizar, sintetizar, plantear hipótesis, tomar decisiones y comunicar las mismas, para dar respuestas pertinentes a las incertidumbres que Erika plantea ante su situación odontológica. Los estudiantes, de una forma dinámica y cooperativa, deberán definir los objetivos de aprendizaje que les permita adquirir los conocimientos, de un modo autodirigido, para dar solución, no sólo a la molestia dental de la protagonista del problema, sino también a sus incertidumbres relacionadas con las pruebas radiológicas que le han de realizar. El problema permite afrontar situaciones técnicas, clínicas y éticas de la práctica odontológica relacionado con la utilización de los rayos X e interpretación de la imagen en odontologí

Las Tecnologías de la Información y Comunicación en la Docencia Universitaria Presencial. Aplicación en Distintas Titulaciones y Áreas de Conocimiento

El objetivo fundamental del presente estudio es analizar el impacto de la aplicación de las Tecnologías de la Información y Comunicación (TICs) como elemento didáctico de autoaprendizaje y facilitador motivacional en la docencia universitaria presencial. El trabajo empírico realizado consiste en la aplicación de distintas herramientas académicas en ocho asignaturas de seis titulaciones de las siguientes áreas de conocimiento: Ciencias de la Salud, Ciencias Naturales, Ciencias Sociales e Ingeniería y Tecnología.motivación, docencia universitaria, análisis de correspondencias, clasificación

H2O2-preconditioned human adipose-derived stem cells (HC016) increase theirresistance to oxidative stress byoverexpressing Nrf2 and bioenergetic adaptation

BackgroundMesenchymal stem cells, including those derived from human adipose tissue (hASCs), are currently being widely investigated for cell therapy. However, when transplanted at the site of injury, the survival and engraftment rates of hASCs are low, mainly due to the harsh microenvironment they encounter, characterized by inflammation and oxidative stress. To overcome these therapeutic limitations, cell preconditioning with low-concentration of hydrogen peroxide (H2O2) has been proposed as a plausible strategy to increase their survival and adaptation to oxidative stress. Nonetheless, the underlying mechanisms of this approach are not yet fully understood. In this study, we analyzed molecular and bioenergetic changes that take place in H2O2 preconditioned hASCs.MethodsLong-term exposure to a low concentration of H2O2 was applied to obtain preconditioned hASCs (named HC016), and then, their response to oxidative stress was analyzed. The effect of preconditioning on the expression of Nrf2 and its downstream antioxidant enzymes (HO-1, SOD-1, GPx-1, and CAT), and of NF-kappa B and its related inflammatory proteins (COX-2 and IL-1 beta), were examined by Western blot. Finally, the Seahorse XF96 Flux analysis system was used to evaluate the mitochondrial respiration and glycolytic function, along with the total ATP production.ResultsWe found that under oxidative conditions, HC016 cells increased the survival by (i) decreasing intracellular ROS levels through the overexpression of the transcription factor Nrf2 and its related antioxidant enzymes HO-1, SOD-1, GPx-1, and CAT; (ii) reducing the secretion of pro-inflammatory molecules COX-2 and IL-1 beta through the attenuation of the expression of NF-kappa B; and (iii) increasing the total ATP production rate through the adaption of their metabolism to meet the energetic demand required to survive.ConclusionsH(2)O(2) preconditioning enhances hASC survival under oxidative stress conditions by stimulating their antioxidant response and bioenergetic adaptation. Therefore, this preconditioning strategy might be considered an excellent tool for strengthening the resistance of hASCs to harmful oxidative stress.Partial funding for this project was provided by the Department of Economic Development and Competitiveness of the Basque Government, the European Regional Development Fund (PREMISE IG-2015/0000558), and the University of the Basque Country (UPV/EHU; research grants PES 17/29 and 16/37)

Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo

Partial hepatectomy (PHx) is the gold standard for the treatment of colorectal cancer liver metastases. However, after removing a substantial amount of hepatic tissue, growth factors are released to induce liver regeneration, which may promote the proliferation of liver micrometastases or circulating tumour cells still present in the patient. The aim of this study is to assess the effect of PHx on the growth of liver metastases induced by intrasplenic cell inoculation as well as on in vitro proliferation of the same cancer cell line. Liver tumours were induced in 18 WAG/RijHsd male rats, by seeding 250,000 syngeneic colorectal cancer cells (CC531) into the spleen. The left lateral lobe of the liver was mobilized and in half of the animals it was removed to achieve a 40% hepatectomy. Twenty-eight days after tumour induction, the animals were sacrificed and the liver was removed and sliced to assess the relative tumour surface area (RTSA%). CC531 cells were cultured in presence of foetal calf serum, non-hepatectomised (NRS) or hepatectomized rat serum (HRS), and their proliferation rate at 24, 48, and 72h was measured. RTSA% was significantly higher in animals which had undergone PHx than in the controls (non-hepatectomised) (46.98±8.76% vs. 18.73±5.65%; p<0.05). Analysing each lobe separately, this difference in favour of hepatectomized animals was relevant and statistically significant in the paramedian and caudate lobes. But in the right lobe the difference was scarce and not significant. In vitro, 2.5% HRS achieved stronger proliferative rates than the control cultures (10% FCS) or their equivalent of NRS. In this experimental model, a parallelism has been shown between the effect of PHx on the growth of colorectal cancer cells in the liver and the effect of the serum on those cells in vitro.This project has been partially funded by the Scientific Foundation of the Spanish Association Against Cancer (AECC) and the Basque Association for Surgical Research (AVEFIQ)

Hydrogen Peroxide-Preconditioned Human Adipose-Derived Stem Cells Enhance the Recovery of Oligodendrocyte-Like Cells after Oxidative Stress-Induced Damage

Oxidative stress associated with neuroinflammation is a key process involved in the pathophysiology of neurodegenerative diseases, and therefore, has been proposed as a crucial target for new therapies. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for neuroprotection. These cells can be preconditioned by exposing them to mild stress in order to improve their response to oxidative stress. In this study, we evaluate the therapeutic potential of hASCs preconditioned with low doses of H2O2 (called HC016 cells) to overcome the deleterious effect of oxidative stress in an in vitro model of oligodendrocyte-like cells (HOGd), through two strategies: i, the culture of oxidized HOGd with HC016 cell-conditioned medium (CM), and ii, the indirect co-culture of oxidized HOGd with HC016 cells, which had or had not been exposed to oxidative stress. The results demonstrated that both strategies had reparative effects, oxidized HC016 cell co-culture being the one associated with the greatest recovery of the damaged HOGd, increasing their viability, reducing their intracellular reactive oxygen species levels and promoting their antioxidant capacity. Taken together, these findings support the view that HC016 cells, given their reparative capacity, might be considered an important breakthrough in cell-based therapies.Partial funding for this project was provided by the Department of Economic Development and Competitiveness of the Basque Government, the European Regional Development Fund (PREMISE IG-2015/0000558) and the University of the Basque Country (UPV/EHU; research grants GIU 19/088 and PES 17/29 and 16/37)

Las Tecnologías de la Información y Comunicación en la Docencia Universitaria Presencial. Aplicación en Distintas Titulaciones y Áreas de Conocimiento

El objetivo fundamental del presente estudio es analizar el impacto de la aplicación de las Tecnologías de la Información y Comunicación (TICs) como elemento didáctico de autoaprendizaje y facilitador motivacional en la docencia universitaria presencial. El trabajo empírico realizado consiste en la aplicación de distintas herramientas académicas en ocho asignaturas de seis titulaciones de las siguientes áreas de conocimiento: Ciencias de la Salud, Ciencias Naturales, Ciencias Sociales e Ingeniería y Tecnología.Este trabajo ha sido subvencionado por el Vicerrectorado de Innovación Docente de la UPV/EHU, proyectos: PIE 19/2004 y PIE 27/2005. Este trabajo ha sido además parcialmente financiado por el Grupo de Investigación Consolidado UPV 038.321- 13631/2001

Conditioned Medium from H2O2-Preconditioned Human Adipose-Derived Stem Cells Ameliorates UVB-Induced Damage to Human Dermal Fibroblasts

Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidative modifications of cellular components and biomolecules involved in the metabolism of dermal cells. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for photoprotection due to their pro-angiogenic properties, protective activity against oxidative stress and paracrine effect on dermal cells. To enhance these therapeutic properties, hASCs can be preconditioned by exposing them to sublethal cellular stressors. In this study, we first analyzed response capacity against UVB-induced oxidative stress in H2O2-preconditioned hASCs (called HC016 cells); and second, we evaluated the photoprotective effect of HC016-conditioned medium (CM) in an in vitro UVB irradiation model in cultured human foreskin fibroblasts (hFFs). The results demonstrated that HC016 cells have a greater capacity to respond efficiently to UVB-induced oxidative stress, evidenced by higher Nrf2 antioxidant system activity and enhanced viability and migration capacity. Further, HC016-CM treatment increased viability, migratory capacity and collagen type I synthesis in hFFs exposed to UVB radiation, as well as reducing their cytotoxicity, apoptosis, senescence and IL-6 secretion. Collectively, these findings support the view that HC016 cells could protect against UVB-induced photodamage via paracrine mechanisms.This research was founded by the University of the Basque Country (UPV/EHU, grant reference numbers: GIU19/088 and PES 21/50)

The information and communication technologies in the university education : influence on students' motivation, self-learning and active participation

[Es] Este estudio analiza la opinión de los alumnos de diferentes licenciaturas sobre la utilidad didáctica de las tecnologías de la información y comunicación (TICs) en la universidad. Se utilizaron páginas web y el correo electrónico para facilitar diferentes herramientas educativas (guiones docentes, artículos, páginas web, trabajos prácticos y bibliografía). Los análisis univariante y multivariante de los datos obtenidos de las encuestas realizadas a los estudiantes al inicio y final de la asignatura, demuestran que, con independencia de la titulación, el 64% del alumnado considera que la utilización de las TICs mejora la comunicación alumno – profesor, e incrementa la motivación y la participación activa del estudiante.[En] This work analyzes the opinion of students, attending different degrees, on the didactic utility of information and communication technologies (ICTs) at the university. Web pages and e-mail were used to facilitate different educational tools (educational scripts, articles, web pages, practical classworks and references). The univariate and multivariate statistical analyses of data from surveys carried out on the students at both, the beginning and the end of the lecture period in a given term, demonstrate that, with regardless of the degree, 64% of the student body considers that the use of the ICTs improves the teacher – student communication, and it increases the students' motivation and their active participation.Este estudio ha sido realizado gracias a la subvención de dos proyectos de innovación educativa (PIE 19/2004 y PIE 27/2005) por parte del Vicerrectorado de Calidad e Innovación docente de la Universidad del País Vasco/EHU. Los autores agradecen el apoyo del Grupo de Investigación Consolidado de la Universidad del País Vasco:UPV 038.321-13631/2001