46 research outputs found
RNA interference can be used to disrupt gene function in tardigrades
How morphological diversity arises is a key question in evolutionary developmental biology. As a long-term approach to address this question, we are developing the water bear Hypsibius dujardini (Phylum Tardigrada) as a model system. We expect that using a close relative of two well-studied models, Drosophila (Phylum Arthropoda) and Caenorhabditis elegans (Phylum Nematoda), will facilitate identifying genetic pathways relevant to understanding the evolution of development. Tardigrades are also valuable research subjects for investigating how organisms and biological materials can survive extreme conditions. Methods to disrupt gene activity are essential to each of these efforts, but no such method yet exists for the Phylum Tardigrada. We developed a protocol to disrupt tardigrade gene functions by double-stranded RNA-mediated RNA interference (RNAi). We show that targeting tardigrade homologs of essential developmental genes by RNAi produced embryonic lethality, whereas targeting green fluorescent protein did not. Disruption of gene functions appears to be relatively specific by two criteria: targeting distinct genes resulted in distinct phenotypes that were consistent with predicted gene functions, and by RT-PCR, RNAi reduced the level of a target mRNA and not a control mRNA. These studies represent the first evidence that gene functions can be disrupted by RNAi in the phylum Tardigrada. Our results form a platform for dissecting tardigrade gene functions for understanding the evolution of developmental mechanisms and survival in extreme environments
Comparative genomics of the tardigrades <i>Hypsibius dujardini</i> and <i>Ramazzottius varieornatus</i>
Tardigrada, a phylum of meiofaunal organisms, have been at the center of discussions of the evolution of Metazoa, the biology of survival in extreme environments, and the role of horizontal gene transfer in animal evolution. Tardigrada are placed as sisters to Arthropoda and Onychophora (velvet worms) in the superphylum Panarthropoda by morphological analyses, but many molecular phylogenies fail to recover this relationship. This tension between molecular and morphological understanding may be very revealing of the mode and patterns of evolution of major groups. Limnoterrestrial tardigrades display extreme cryptobiotic abilities, including anhydrobiosis and cryobiosis, as do bdelloid rotifers, nematodes, and other animals of the water film. These extremophile behaviors challenge understanding of normal, aqueous physiology: how does a multicellular organism avoid lethal cellular collapse in the absence of liquid water? Meiofaunal species have been reported to have elevated levels of horizontal gene transfer (HGT) events, but how important this is in evolution, and particularly in the evolution of extremophile physiology, is unclear. To address these questions, we resequenced and reassembled the genome of H. dujardini, a limnoterrestrial tardigrade that can undergo anhydrobiosis only after extensive pre-exposure to drying conditions, and compared it to the genome of R. varieornatus, a related species with tolerance to rapid desiccation. The 2 species had contrasting gene expression responses to anhydrobiosis, with major transcriptional change in H. dujardini but limited regulation in R. varieornatus. We identified few horizontally transferred genes, but some of these were shown to be involved in entry into anhydrobiosis. Whole-genome molecular phylogenies supported a Tardigrada+Nematoda relationship over Tardigrada+Arthropoda, but rare genomic changes tended to support Tardigrada+Arthropoda
Linking PAR polarity proteins to cell fate regulation: analysis of MEX-5 localization in Caenorhabditis elegans embryos
Thesis (Ph. D.)--University of Washington, 2007.Specification of somatic and germline lineages in the nematode Caenorhabditis elegans requires the establishment of anterior-posterior polarity in early embryos. Polarization begins by a sperm-induced cue in 1-cell embryos that cortically localizes PAR polarity proteins, including the Ser/Thr kinases PAR-1 and PAR-4/LKB1. Capping at the anterior pole of non-muscle myosin and several PAR proteins leads to asymmetric localization of proteins such as MEX-5 and MEX-6. MEX-5,-6 are closely-related CCCH zinc finger proteins required for germline specification that are anteriorly localized in 1-cell embryos. While no direct targets of the PAR proteins have been described in C. elegans, MEX-5,-6 are proposed to function as key intermediaries in the transduction of polarity cues from PAR proteins to downstream cell fate regulators. To understand how MEX-5 asymmetry is established, I constructed a series of fusion proteins containing Green Fluorescent Protein (GFP) fused to all or part of the MEX-5 protein; these fusion proteins allowed me to monitor asymmetry in living embryos. Deletion analysis of GFP:MEX-5 identified a single residue, Ser458, that is necessary for anterior localization of GFP:MEX-5 in 1-cell embryos. MEX-5 is phosphorylated at Ser458 in vivo, and this phosphorylation occurs at the onset of MEX-5 expression in the gonad. In a screen of 41 Ser/Thr kinases, I found that only PAR-1 and PAR-4 are necessary for MEX-5 phosphorylation. PAR-1 kinase activity is required for the initial phosphorylation of MEX-5, as a kinase-dead allele of par-1 abolished all staining with anti-MEX-5(pSer458) in oocytes and embryos. PAR-4 kinase activity is required to maintain MEX-5 phosphorylation in mature oocytes; anti-MEX-5(pSer458) staining decreased progressively in par-4 mutant oocytes, and was present only at low levels in 1-cell embryos. While phosphorylation of MEX-5 is necessary for its asymmetry in 1-cell embryos, it is not sufficient; in par-1 alleles with mutations outside the kinase domain, MEX-5 was phosphorylated but remained symmetric. In summary, my research has described multiple roles for the PAR-1 and PAR-4 kinases in MEX-5 phosphorylation and localization. These results provide the first evidence of a role for the PAR kinases in setting up embryonic asymmetries prior to fertilization
23andMe and YOU: What Can You Learn from Your Genome (and Do You Really Want to Know?)
The last decade has seen a sharp rise in “direct-to-consumer” genetic testing, allowing anyone (and their dogs!) the opportunity to sequence their genome. Depending on the company and product you choose, you can explore your ancestry and ethnicity or identify risk factors for specific diseases. However, concerns have been raised about the privacy and security of collected data, the accuracy of disease-risk analysis and the ethics of revealing these data in the absence of expert genetic counseling. In this workshop, we will describe the types of information that an exploration of your genome can reveal, then discuss the social, ethical, legal and personal ramifications of having access to our genetic data
RNA interference can be used to disrupt gene function in tardigrades
Abstract How morphological diversity arises is a key question in evolutionary developmental biology. As a long-term approach to address this question, we are developing the water bear Hypsibius dujardini (Phylum Tardigrada) as a model system. We expect that using a close relative of two well-studied models, Drosophila (Phylum Arthropoda) and Caenorhabditis elegans (Phylum Nematoda), will facilitate identifying genetic pathways relevant to understanding the evolution of development. Tardigrades are also valuable research subjects for investigating how organisms and biological materials can survive extreme conditions. Methods to disrupt gene activity are essential to each of these efforts, but no such method yet exists for the Phylum Tardigrada. We developed a protocol to disrupt tardigrade gene functions by double-stranded RNA-mediated RNA interference (RNAi). We showed that targeting tardigrade homologs of essential developmental genes by RNAi produced embryonic lethality, whereas targeting green fluorescent protein did not. Disruption of gene functions appears to be relatively specific by two criteria: targeting distinct genes resulted in distinct phenotypes that were consistent with predicted gene functions and by RT-PCR, RNAi reduced the level of a target mRNA and not a control mRNA. These studies represent the first evidence that gene functions can be disrupted by RNAi in the phylum Tardigrada. Our results form a platform for dissecting tardigrade gene functions for understanding the evolution of developmental mechanisms and survival in extreme environments
MEX-5 enrichment in the C. elegans early embryo mediated by differential diffusion
Specification of germline and somatic cell lineages in C. elegans originates in the polarized single-cell zygote. Several cell-fate determinants are partitioned unequally along the anterior-posterior axis of the zygote, ensuring the daughter cells a unique inheritance upon asymmetric cell division. Recent studies have revealed that partitioning of the germline determinant PIE-1 and the somatic determinant MEX-5 involve protein redistribution accompanied by spatiotemporal changes in protein diffusion rates. Here, we characterize the dynamics of MEX-5 in the zygote and propose a novel reaction/diffusion model to explain both its anterior enrichment and its remarkable intracellular dynamics without requiring asymmetrically distributed binding sites. We propose that asymmetric cortically localized PAR proteins mediate the anterior enrichment of MEX-5 by reversibly changing its diffusion rate at spatially distinct points in the embryo, thus generating a stable concentration gradient along the anterior-posterior axis of the cell. This work extends the scope of reaction/diffusion models to include not only germline morphogens, but also somatic determinants