[BI019] Molecular Cloning And Characterisation Of The 5 -Untranslated Region And Promoters In Human Peroxisome Proliferator-Activated Receptor Alpha (hPPARα).

The peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor gene superfamily of ligandactivated transcription factors [Issemann and Green, 1990]

Cytotoxic Activities of Physalis minima L. Chloroform Extract on Human Lung Adenocarcinoma NCI-H23 Cell Lines by Induction of Apoptosis

Physalis minima L. is reputed for having anticancer property. In this study, the chloroform extract of this plant exhibited remarkable cytotoxic activities on NCI-H23 (human lung adenocarcinoma) cell line at dose- and time-dependent manners (after 24, 48 and 72 h of incubation). Analysis of cell-death mechanism demonstrated that the extract exerted apoptotic programed cell death in NCI-H23 cells with typical DNA fragmentation, which is a biochemical hallmark of apoptosis. Morphological observation using transmission electron microscope (TEM) also displayed apoptotic characteristics in the treated cells, including clumping and margination of chromatins, followed by convolution of the nuclear and budding of the cells to produce membrane-bound apoptotic bodies. Different stages of apoptotic programed cell death as well as phosphatidylserine externalization were confirmed using annexin V and propidium iodide staining. Furthermore, acute exposure to the extract produced a significant regulation of c-myc, caspase-3 and p53 mRNA expression in this cell line. Due to its apoptotic effect on NCI-H23 cells, it is strongly suggested that the extract could be further developed as an anticancer drug

Tissue Expression Of The 6-Pyruvoyi Tetrahydropterin Synthase Gene In Adult Zebrafish,Danio Rerio.

Teleost is one of the poikilotherms that can display different kind of colours on their body. This is due to the presence of different types of chromatophores, a group of pigment containing cells that is distributed in the epidermal layer of skin and scales

A new 4’,6-dihydroxy,4-methoxybenzophenone-2-O-β-D-gentiobioside and 4’,6-dihydroxy,4 methoxybenzophenone-2-O-β-D-glucoside isolated from methanolic extract of Phaleria macrocarpa (Scheff.) Boerl leaves

A new 4’,6-dihydroxy,4-methoxybenzophenone-2-O-β-D-gentiobioside (1) was obtained from the leaves of Phaleria macrocarpa (Scheff.) Boerl along with known 4’,6-dihydroxy,4-methoxybenzophenone-2-O-β-D-glucoside (2). Bioactivity assays indicated that compound (1) and (2) have antioxidant activity and enhancement on SR-B1 genes expression in vitro. Their structures compounds were established by the interpretation of their spectroscopic data and comparison with known compounds

Molecular cloning and characterisation of the 5’-untranslated region and promoters in human peroxisome proliferator-activated receptor alpha (hPPARα)

The peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor gene superfamily of ligandactivated transcription factors [Issemann and Green, 1990]

Preliminary in vitro cytotoxic assay of human liver carcinoma cells (HepG2) of organotin(IV) complexes: Synthesis and characterization of organotin(IV) complexes of 2,4-dinitrobenzoic and 3,5-dinitrobenzoic acids

A total of five organotin(IV) carboxylate complexes was successfully synthesized and characterized quantitatively and qualitatively. Results of the infrared spectroscopy of the parent acids and complexes showed that the coordination took place via oxygen atoms from the carboxylate group. From the preliminary in vitro cytotoxic assay study, triorganotin(IV) complexes (2 and 5) were found to exhibit better activity as compared to diorganotin(IV) complexes (1, 3 and 4) but lower activity as compared to the reference drug. In addition, within the diorganotin(IV) complexes, monomeric type (3) exhibited a slightly better activity as compared to the organodistannoxane dimer types (1 and 4).Keywords: Preliminary in vitro cytotoxic assay, organotin(IV) complexes, comparison stud

A new 4’,6-dihydroxy,4-methoxybenzophenone-2-O-β-D-gentiobioside and 4’,6-dihydroxy,4 methoxybenzophenone-2-O-β-D-glucoside isolated from methanolic extract of Phaleria macrocarpa (Scheff.) Boerl leaves

A new 4’,6-dihydroxy,4-methoxybenzophenone-2-O-β-D-gentiobioside (1) was obtained from the leaves of Phaleria macrocarpa (Scheff.) Boerl along with known 4’,6-dihydroxy,4-methoxybenzophenone-2-O-β-D-glucoside (2). Bioactivity assays indicated that compound (1) and (2) have antioxidant activity and enhancement on SR-B1 genes expression in vitro. Their structures compounds were established by the interpretation of their spectroscopic data and comparison with known compounds

Molecular characterization of peroxisome proliferator activated receptor gamma2 (PPARγ2) promoter from bovine

Peroxisome proliferator activated receptor gamma (PPARγ) is a member of the PPAR subfamily of nuclear hormone receptors (Schoonjans et al., 1996a). So far, two PPARγ isoforms; γ1 and γ2, have been cloned in bovine and produced by alternative promoter usage and differential splicing of a single gene (Sundvold et al., 1997). PPARγ functions by forming heterodimeric complexes with 9-cis-retinoic acid X receptors (RXRs). This heterodimer regulates transcription by binding to PPRE, known also as DR-1 response element in the regulatory regions of target genes (Tontonoz et al., 1994a)

THE EFFECT OF MARINE SPONGE Aaptos aaptos EXTRACT IN VIBRIOSIS TREATMENT OF BLACK TIGER SHRIMP Penaeus monodon LARVAE

Black tiger shrimp Penaeus monodon post larvae were challenged with Vibrio harveyi and butanol extract of selected marine sponge Aaptos aaptos to determine its antibacterial bioactive potential in vibriosis treatment. Based on the preliminary toxicity study, the A. aaptos butanol extract with concentrations of 31.25, 62.5, and 125 mg/L were selected in the study. Black tiger shrimp post larvae were challenged with V. harveyi at 107 cfu/mL and immersed A. aaptos butanol extract with the concentration of 125 mg/L showed significantly in (P<0.05) decrease mortality of the post larvae treated. Besides at this concentration, V. harveyi population in the rearing water and the post larvae treated decreased compared to control (untreated post larvae). Histological observation indicated that there was no changing on hepatopancreas of the black tiger shrimp post larvae. Based on this result, it is suggested that the butanol extract of A. aaptos is a potential bioactive compounds source in the treatment of vibriosis which may replaced the current antibiotics application

Biosynthetic production of human growth hormone gene in methylotrophic yeast, Pichia pastoris

Human growth hormone (hGH) is secreted from the anterior pituitary gland and exerts a wide variety of functions such as, IGF-1 production, protein synthesis, glucose metabolism, lipolysis, lipogenesis, and cell proliferation/differentiation (Isaksson et al., 1985; Press, 1988; Thorner and Vance, 1988; Strobl and Thomas, 1994)