<i>Anopheles stephensi</i> as an emerging malaria vector in the Horn of Africa with high susceptibility to Ethiopian <i>Plasmodium vivax</i> and <i>Plasmodium falciparum</i> isolates

AbstractAnopheles stephensi, an efficient Asian malaria vector, recently spread into the Horn of Africa and may increase malaria receptivity in African urban areas. We assessed occurrence, genetic complexity, blood meal source and infection status of An. stephensi in Awash Sebat Kilo town, Ethiopia. We used membrane feeding assays to assess competence of local An. stephensi to P. vivax and P. falciparum isolates from clinical patients. 75.3% of the examined waterbodies were infested with An. stephensi developmental stages that were genetically closely related to isolates from Djibouti and Pakistan. Both P. vivax and P. falciparum were detected in wild-caught adult An. stephensi. Local An. stephensi was more receptive to P. vivax compared to a colony of An. arabiensis. We conclude that An. stephensi is an established vector in this part of Ethiopia, highly permissive for local P. vivax and P. falciparum isolates and presents an important new challenge for malaria control.Summary of the articleAn. stephensi, a metropolitan malaria vector that recently expanded to the Horn of African, was highly susceptible to local P. falciparum and P. vivax isolates from Ethiopia and may increase malariogenic potential of rapidly expanding urban settings in Africa.</jats:sec

Test cross performance and combining ability of newly introduced quality protein maize (Zea mays) inbred lines for grain yield and agronomic traits evaluated in mid-altitude agro-ecological zones of Ethiopia

Development of quality protein maize (QPM) helps to reduce human malnutrition in areas where maize is the major protein source in the diet. The objective of this study was to estimate combining ability effects of QPM inbred lines for grain yield and yield-related traits. Twenty-one newly introduced QPM inbred lines were crossed with four single cross testers in a line × tester mating design and resulted in 84 F1 progenies. These F1 hybrids, including four checks, were evaluated in an 8 × 11 alpha lattice design with two replications at four locations during 2016. Mean squares due to location, crosses, general combining ability (GCA) and specific combining ability (SCA) were significant for most traits. However, the contributions of GCA sum of squares to the variation among the hybrids were larger than SCA sum of squares, suggesting that the traits were controlled predominantly by additive gene effects. Among the inbred lines, L3, L5 and L10 showed positive and significant GCA effects for grain yield and other agronomic traits. The lines can be used in synthetic variety development. Among crosses, only L3 × T3, L9 × T1 and L15 × T2 revealed positive and significant SCA effects for grain yield, implying the presence of heterosis in these particular crosses

Fast-Tracking the Development and Dissemination of a Drought-Tolerant Maize Variety in Ethiopia in Response to the Risks of Climate Change

Climate change projections suggest increased frequency of drought in many parts of sub-Saharan Africa (SSA). The replacement of old varieties of maize with new drought-tolerant (DT) varieties will be crucial to respond to the future risk of drought, as it already is today. The first group of locally developed maize hybrids in Ethiopia—BH140, BH660 and BH540—were commercialised between 1988 and 1995, but were not selected for drought tolerance. Among these, BH660 remained the most popular and widely grown maize variety in the Ethiopian maize belt between 2000 and 2010, accounting for nearly 50% of maize area under improved seed. A new DT hybrid, BH661, with better agronomic performances under optimum and random drought than BH660, was identified and released in 2011. In 2016, 9000 tonnes of certified seed—enough to plant 360,000 ha—was produced and marketed. The concerted effort of breeders and seed producers as well as governmental and non-governmental extension workers drove the development, release and rapid adoption of BH661 contributing to food and income security of more than 300,000 households by mitigating the effects of climate change in Ethiopia. The success of BH661 is a valuable and timely case study for breeders, seed companies, extension agents, regulatory bodies and policy-makers striving to develop and disseminate new DT varieties in sub-Saharan Africa

Additional file 4: of Genetic variation and population structure of maize inbred lines adapted to the mid-altitude sub-humid maize agro-ecology of Ethiopia using single nucleotide polymorphic (SNP) markers

Heat map of the 265 inbred lines based on relative kinship matrix estimated from 220,878 polymorphic SNPs. (DOCX 211 kb

An update on the distribution, bionomics, and insecticide susceptibility of Anopheles stephensi in Ethiopia, 2018–2020

Abstract Background Anopheles stephensi, an invasive malaria vector, was first detected in Africa nearly 10 years ago. After the initial finding in Djibouti, it has subsequently been found in Ethiopia, Sudan and Somalia. To better inform policies and vector control decisions, it is important to understand the distribution, bionomics, insecticide susceptibility, and transmission potential of An. stephensi. These aspects were studied as part of routine entomological monitoring in Ethiopia between 2018 and 2020. Methods Adult mosquitoes were collected using human landing collections, pyrethrum spray catches, CDC light traps, animal-baited tent traps, resting boxes, and manual aspiration from animal shelters. Larvae were collected using hand-held dippers. The source of blood in blood-fed mosquitoes and the presence of sporozoites was assessed through enzyme-linked immunosorbent assays (ELISA). Insecticide susceptibility was assessed for pyrethroids, organophosphates and carbamates. Results Adult An. stephensi were collected with aspiration, black resting boxes, and animal-baited traps collecting the highest numbers of mosquitoes. Although sampling efforts were geographically widespread, An. stephensi larvae were collected in urban and rural sites in eastern Ethiopia, but An. stephensi larvae were not found in western Ethiopian sites. Blood-meal analysis revealed a high proportion of blood meals that were taken from goats, and only a small proportion from humans. Plasmodium vivax was detected in wild-collected An. stephensi. High levels of insecticide resistance were detected to pyrethroids, carbamates and organophosphates. Pre-exposure to piperonyl butoxide increased susceptibility to pyrethroids. Larvae were found to be susceptible to temephos. Conclusions Understanding the bionomics, insecticide susceptibility and distribution of An. stephensi will improve the quality of a national response in Ethiopia and provide additional information on populations of this invasive species in Africa. Further work is needed to understand the role that An. stephensi will have in Plasmodium transmission and malaria case incidence. While additional data are being collected, national programmes can use the available data to formulate and operationalize national strategies against the threat of An. stephensi

Additional file 1: of Genetic variation and population structure of maize inbred lines adapted to the mid-altitude sub-humid maize agro-ecology of Ethiopia using single nucleotide polymorphic (SNP) markers

Summary of the 265 inbred lines and population structure. (XLSX 47 kb