Designing Audience-Centered Interactive Voice Response Messages to Promote Cancer Screenings Among Low-Income Latinas

Introduction: Cancer screening rates among Latinas are suboptimal. The objective of this study was to explore how Latinas perceive cancer screening and the use and design of interactive voice response (IVR) messages to prompt scheduling of 1 or more needed screenings. Methods: Seven focus groups were conducted with Latina community health center patients (n = 40) in need of 1 or more cancer screenings: 5 groups were of women in need of 1 cancer screening (breast, cervical, or colorectal), and 2 groups were of women in need of multiple screenings. A bilingual researcher conducted all focus groups in Spanish using a semistructured guide. Focus groups were recorded, transcribed, and translated into English for analysis. Emergent themes were identified by using thematic content analysis. Results: Participants were familiar with cancer screening and viewed it positively, although barriers to screening were identified (unaware overdue for screening, lack of physician referral, lack of insurance or insufficient insurance coverage, embarrassment or fear of screening procedures, fear of screening outcomes). Women needing multiple screenings voiced more concern about screening procedures, whereas women in need of a single screening expressed greater worry about the screening outcome. Participants were receptive to receiving IVR messages and believed that culturally appropriate messages that specified needed screenings while emphasizing the benefit of preventive screening would motivate them to schedule needed screenings. Conclusion: Participants’ receptiveness to IVR messages suggests that these messages may be an acceptable strategy to promote cancer screening among underserved Latina patients. Additional research is needed to determine the effectiveness of IVR messages in promoting completion of cancer screening

Methodology of a diabetes prevention translational research project utilizing a community-academic partnership for implementation in an underserved Latino community

<p>Abstract</p> <p>Background</p> <p>Latinos comprise the largest racial/ethnic group in the United States and have 2–3 times the prevalence of type 2 diabetes mellitus as Caucasians.</p> <p>Methods and design</p> <p>The Lawrence Latino Diabetes Prevention Project (LLDPP) is a community-based translational research study which aims to reduce the risk of diabetes among Latinos who have a ≥ 30% probability of developing diabetes in the next 7.5 years per a predictive equation. The project was conducted in Lawrence, Massachusetts, a predominantly Caribbean-origin urban Latino community. Individuals were identified primarily from a community health center's patient panel, screened for study eligibility, randomized to either a usual care or a lifestyle intervention condition, and followed for one year. Like the efficacious Diabetes Prevention Program (DPP), the LLDPP intervention targeted weight loss through dietary change and increased physical activity. However, unlike the DPP, the LLDPP intervention was less intensive, tailored to literacy needs and cultural preferences, and delivered in Spanish. The group format of the intervention (13 group sessions over 1 year) was complemented by 3 individual home visits and was implemented by individuals from the community with training and supervision by a clinical research nutritionist and a behavioral psychologist. Study measures included demographics, Stern predictive equation components (age, gender, ethnicity, fasting glucose, systolic blood pressure, HDL-cholesterol, body mass index, and family history of diabetes), glycosylated hemoglobin, dietary intake, physical activity, depressive symptoms, social support, quality of life, and medication use. Body weight was measured at baseline, 6-months, and one-year; all other measures were assessed at baseline and one-year. All surveys were orally administered in Spanish.</p> <p>Results</p> <p>A community-academic partnership enabled the successful recruitment, intervention, and assessment of Latinos at risk of diabetes with a one-year study retention rate of 93%.</p> <p>Trial registration</p> <p>NCT00810290</p

Awareness of diabetes risk factors and prevention strategies among a sample of low-income Latinos with no known diagnosis of diabetes

PURPOSE: This study assessed awareness of type 2 diabetes risk and severity, perceived risk factors, knowledge of diabetes prevention strategies, and challenges of and opportunities for prevention among low-income Latinos in Lawrence, Massachusetts. METHODS: Qualitative research design. Latinos with no known diagnosis of diabetes participated in 4 focus groups, conducted in Spanish, which were recorded and transcribed for systematic analysis. RESULTS: The sample, (N = 41) was largely female (85%) with a wide age range (22-76 years), most (71%) had an educational level of high school or less, and less than half (46%) were employed. Participants had basic knowledge of diabetes, but gaps were apparent. Many perceived family history of diabetes, poor diet, emotional distress, and stress associated with the United States as risk factors for diabetes. There was little or no awareness of risk associated with Latino ethnicity, gestational diabetes, hypertension, lipid abnormalities, or obesity. Few cited physical activity or weight loss as diabetes prevention strategies. More than half the participants perceived themselves at low risk for diabetes. CONCLUSIONS: This Latino sample had limited knowledge of diabetes risk factors and lifestyle changes that can prevent or delay diabetes onset. Insights for intervening for diabetes prevention are offered

Bioactive Phytochemicals from Mercurialis spp. Used in Traditional Spanish Medicine

Plants from the genus Mercurialis have a long history of use as herbal remedies in traditional Spanish medicine. The growing interest in the conservation of knowledge related to biodiversity has encouraged us to review the bioactive phytochemicals from the four most widespread Mercurialis species in the Iberian Peninsula (M. annua L., M. ambigua L., M. perennis L., and M. tomentosa L.). First, the medicinal uses of these four species throughout Spain were compiled, and then a bibliographical search on their chemical composition was conducted in an attempt to justify their reported traditional uses. We found that most of the medicinal uses of Mercurialis spp. are supported by scientific evidence. This includes its antidiabetic and antihypertensive properties attributable to the flavonoid rutin and narcissin, respectively; its benefits in the treatment of skin dark spots, attributable to mequinol; and its anti-inflammatory activity, attributable to scopoletin, kaempferol, squalene, and cycloartenol. This review contributes to the validation of the medicinal uses of Mercurialis spp. in Spain and provides some new avenues for further investigations on the biological activity of this interesting medicinal plant

Eating disorders diagnostic: From the DSM-IV to DSM-5 / El diagnóstico de los trastornos alimentarios del DSM-IV-TR al DSM-5

Abstract The purpose of this study is to analyze the changes in the eating disorders diagnostic criteria and its implications for clinical and research work. Comparison tables were done for each eating disorder included in DSM-5: pica, rumination, anorexia nervosa, bulimia nervosa, avoidant /restrictive food intake disorder, binge eating disorder, other feeding and eating disorder specified and unspecified. As a conclusion important conceiving changes can be obser- ved, as in the onset time, therefore its definition considers feeding behavior, and the absorption of food problems were added, as well as worsening areas are specified. Particulary the avoidant /restrictive food intake disorder and anorexia nervosa are those that more changes present. Resumen El presente trabajo tiene por objetivo analizar los cambios del DSM-IV-TR al DSM-5 en los trastornos alimentarios, en cuanto a sus criterios diagnósticos y sus implicaciones en el ámbito clínico. Para ello se realizaron tablas de comparación para cada uno de los trastornos alimentarios incluidos en el DSM-5: pica, rumiación, trastorno de la evitación/restricción de alimentos, anorexia nerviosa, bulimia nerviosa, trastorno de atracones, otro trastorno de la conducta alimentaria o de la ingesta de alimentos especificado y no especificado. En conclusión podemos observar importantes cambios en su concepción, como en el tiempo de inicio, por ende su definición considera a la conducta alimentaria y an ̃ade los problemas en la absorción de los alimentos, además de explicitar áreas de deterioro. En particular, los trastornos de la evitación/restricción de alimentos y anorexia nerviosa son los que mayores cambios presentan

Association of depressive symptoms and lifestyle behaviors among Latinos at risk of type 2 diabetes

Little is known about depression among Latinos at risk for type 2 diabetes. The purpose of this cross-sectional study was to determine the rate of depression in Latinos at risk for type 2 diabetes and to examine the associations between depressive symptoms, diet, physical activity, and body mass index (BMI; calculated as kg/m(2)). Latinos at risk for type 2 diabetes (n=210) were surveyed from July 2007 to August 2008. Depressive symptoms were assessed using the Center for Epidemiological Studies Depression Scale (CES-D). Three 24-hour recalls were used to assess diet and physical activity. Linear regression analyses were used to examine the association between depression, BMI, and lifestyle factors. The sample (78% female) was largely of Caribbean origin (83%) and mean age was 52 years (standard deviation [SD]=11 years). Mean BMI was 34.2 (SD=5.9) and 77% were obese. Average CES-D score was 16.3 (SD=11.3; range=0-45) and 50% had CES-D score \u3e or =16, suggesting clinical depression. Higher CES-D scores were associated with lower diet quality (P\u3c0.05), but were not associated with BMI or physical activity. Depression and lower dietary quality can contribute to risk for type 2 diabetes among Latinos

Designing audience-centered interactive voice response messages to promote cancer screenings among low-income Latinas.

IntroductionCancer screening rates among Latinas are suboptimal. The objective of this study was to explore how Latinas perceive cancer screening and the use and design of interactive voice response (IVR) messages to prompt scheduling of 1 or more needed screenings.MethodsSeven focus groups were conducted with Latina community health center patients (n = 40) in need of 1 or more cancer screenings: 5 groups were of women in need of 1 cancer screening (breast, cervical, or colorectal), and 2 groups were of women in need of multiple screenings. A bilingual researcher conducted all focus groups in Spanish using a semistructured guide. Focus groups were recorded, transcribed, and translated into English for analysis. Emergent themes were identified by using thematic content analysis.ResultsParticipants were familiar with cancer screening and viewed it positively, although barriers to screening were identified (unaware overdue for screening, lack of physician referral, lack of insurance or insufficient insurance coverage, embarrassment or fear of screening procedures, fear of screening outcomes). Women needing multiple screenings voiced more concern about screening procedures, whereas women in need of a single screening expressed greater worry about the screening outcome. Participants were receptive to receiving IVR messages and believed that culturally appropriate messages that specified needed screenings while emphasizing the benefit of preventive screening would motivate them to schedule needed screenings.ConclusionParticipants' receptiveness to IVR messages suggests that these messages may be an acceptable strategy to promote cancer screening among underserved Latina patients. Additional research is needed to determine the effectiveness of IVR messages in promoting completion of cancer screening

PKC-Dependent GlyT1 Ubiquitination Occurs Independent of Phosphorylation: Inespecificity in Lysine Selection for Ubiquitination

<div><p>Neurotransmitter transporter ubiquitination is emerging as the main mechanism for endocytosis and sorting of cargo into lysosomes. In this study, we demonstrate PKC-dependent ubiquitination of three different isoforms of the glycine transporter 1 (GlyT1). Incubation of cells expressing transporter with the PKC activator phorbol ester induced a dramatic, time-dependent increase in GlyT1 ubiquitination, followed by accumulation of GlyT1 in EEA1 positive early endosomes. This occurred via a mechanism that was abolished by inhibition of PKC. GlyT1 endocytosis was confirmed in both retinal sections and primary cultures of mouse amacrine neurons. Replacement of only all lysines in the <i>N</i>-and <i>C</i>-termini to arginines prevented ubiquitination and endocytosis, displaying redundancy in the mechanism of ubiquitination. Interestingly, a 40–50% reduction in glycine uptake was detected in phorbol-ester stimulated cells expressing the WT-GlyT1, whereas no significant change was for the mutant protein, demonstrating that endocytosis participates in the reduction of uptake. Consistent with previous findings for the dopamine transporter DAT, ubiquitination of GlyT1 tails functions as sorting signal to deliver transporter into the lysosome and removal of ubiquitination sites dramatically attenuated the rate of GlyT1 degradation. Finally, we showed for the first time that PKC-dependent GlyT1 phosphorylation was not affected by removal of ubiquitination sites, suggesting separate PKC-dependent signaling events for these posttranslational modifications.</p></div

Schematic representation of the predicted topology of GlyT1 isoforms and PKC- induced endocytosis of GlyT1.

<p><b>A)</b> The twelve membrane-spanning segments are depicted by cylinders, and intracellular <i>N</i> and <i>C</i> termini, loops and extracellular glycosylation sites by solid lines. The position of lysine residues in the three different <i>N</i>-terminal splice variants are presented by beads (GlyT1a, 1b and 1c). Conserved lysines are highlighted by gray beads. <b>B)</b> PAE cells stably expressing FH-GlyT1b and <b>C)</b> PAE cells stably expressing FH-GlyT1c were incubated with DMSO or PMA for 30–60 min at 37°C, fixed and immunostained with anti-GlyT1 and anti-EAA1 antibodies followed by incubation with a CY-3 and Alexa 488 labeled secondary antibodies. Images were selected to represent the cell population and acquired through YFP (green) and CY3 (red) filter channels. Single optical sections through the middle of the cells are shown. ‘Yellow’ in the merged images signifies co-localization of CY3 (GlyT1) and YFP (EEA1). D) Co-localization was quantified in pixel by pixel bases from images obtained by confocal microscopy using the Mander’s overlap coefficient of merged images. A value of 1 represents 100% co-localization of both fluorescence signals in 15 randomly selected endosomes, whereas a zero value denotes complete absence of co-localization. <i>p</i> values were determine by student’s <i>t</i>-test. <i>Scale bars</i>, 10 μm.</p