58 research outputs found
The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
Purpose: The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. Methods: C57BL/6 female mice were infected using intravitreal injections of either 10 6 tachyzoites of Toxoplasma gondii (RH strain; T. gondii) or PBS only (control groups). After 24, 48, and 72 h, animals were sacrificed and their eyes were harvested for histopathological, immunohistochemical, and ultrastructural immunocytochemical analysis of ANXA1. Human retinal pigment epithelial (RPE) cells (ARPE-19) were infected in vitro with T. gondii and collected after 60, 120, 240 min, and 24 h. Results: Compared with non-infected eyes, an intense inflammatory response was observed in the anterior (24 h after infection) and posterior segments (72 h after infection) of the infected eye, characterized by neutrophil infiltration and by the presence of tachyzoites and their consequent destruction along with disorganization of normal retina architecture and RPE vacuolization. T. gondii infection was associated with a significant increase of ANXA1 expression in the neutrophils at 24, 48, and 72 h, and in the RPE at 48 and 72 h. In vitro studies confirmed an upregulation of ANXA1 levels in RPE cells, after 60 and 120 min of infection with T. gondii. Conclusions: The positive modulation of endogenous ANXA1 in the inflammatory and RPE cells during T. gondii infection suggests that this protein may serve as a therapeutic target in ocular toxoplasmosis. © 2012 Molecular Vision
Casamentos mistos: novas sociabilidades e quadros coletivos. Aspectos da imigração de brasileiras na Itália
Rhizosphere pH and phosphorus forms in an Oxisol cultivated with soybean, brachiaria grass, millet and sorghum
Mitigação das emissões de amônia por zeólitas naturais durante a compostagem de dejetos de suínos
Composição mineral, qualidade e degenerescência de polpa de maçãs 'Fuji' em diferentes porta-enxertos durante armazenamento em atmosfera controlada
Qualidade de maçãs 'Fuji' influenciada pela adubação nitrogenada e potássica em dois tipos de solo
Atividade do herbicida acetochlor em solo submetido à semeadura direta e ao preparo convencional
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