World Handbook of Political Indicators IV

Research project funded for academic years 2006-07, 2007-08, and 2008-09The University Archives has determined that this item is of continuing value to OSU's history.The World Handbook of Political Indicators has been published by Yale University Press since 1963 to provide statistics and data to help scholars studying political processes and political change. While the handbook has been the dominant source for analyzing conflict and violence internationally, data collection for the last edition stopped in 1982. In this fourth edition, Jenkins and his team aim to bring the handbook current to 2003 and make the data available over the Internet. In the process, they have made several revolutionary changes that will prepare the handbook for 21st century research.Mershon Center for International Security StudiesProject summar

Three-dimensional structural modelling and calculation of electrostatic potentials of HLA Bw4 and Bw6 epitopes to explain the molecular basis for alloantibody binding: toward predicting HLA antigenicity and immunogenicity.

BACKGROUND: We have previously shown that qualitative assessment of surface electrostatic potential of HLA class I molecules helps explain serological patterns of alloantibody binding. We have now used a novel computational approach to quantitate differences in surface electrostatic potential of HLA B-cell epitopes and applied this to explain HLA Bw4 and Bw6 antigenicity. METHODS: Protein structure models of HLA class I alleles expressing either the Bw4 or Bw6 epitope (defined by sequence motifs at positions 77 to 83) were generated using comparative structure prediction. The electrostatic potential in 3-dimensional space encompassing the Bw4/Bw6 epitope was computed by solving the Poisson-Boltzmann equation and quantitatively compared in a pairwise, all-versus-all fashion to produce distance matrices that cluster epitopes with similar electrostatics properties. RESULTS: Quantitative comparison of surface electrostatic potential at the carboxyl terminal of the α1-helix of HLA class I alleles, corresponding to amino acid sequence motif 77 to 83, produced clustering of HLA molecules in 3 principal groups according to Bw4 or Bw6 epitope expression. Remarkably, quantitative differences in electrostatic potential reflected known patterns of serological reactivity better than Bw4/Bw6 amino acid sequence motifs. Quantitative assessment of epitope electrostatic potential allowed the impact of known amino acid substitutions (HLA-B*07:02 R79G, R82L, G83R) that are critical for antibody binding to be predicted. CONCLUSIONS: We describe a novel approach for quantitating differences in HLA B-cell epitope electrostatic potential. Proof of principle is provided that this approach enables better assessment of HLA epitope antigenicity than amino acid sequence data alone, and it may allow prediction of HLA immunogenicity.This is the author accepted manuscript. The final version is available from Wolters Kluwer via http://dx.doi.org/10.1097/TP.000000000000054

The LWA1 Radio Telescope

LWA1 is a new radio telescope operating in the frequency range 10-88 MHz, located in central New Mexico. The telescope consists of 258 pairs of dipole-type antennas whose outputs are individually digitized and formed into beams. Simultaneously, signals from all dipoles can be recorded using one of the instrument's "all dipoles" modes, facilitating all-sky imaging. Notable features of the instrument include high intrinsic sensitivity (about 6 kJy zenith system equivalent flux density), large instantaneous bandwidth (up to 78 MHz), and 4 independently-steerable beams utilizing digital "true time delay" beamforming. This paper summarizes the design of LWA1 and its performance as determined in commissioning experiments. We describe the method currently in use for array calibration, and report on measurements of sensitivity and beamwidth.Comment: 9 pages, 14 figures, accepted by IEEE Trans. Antennas & Propagation. Various minor changes from previous versio

The diversity of sulfide oxidation and sulfate reduction genes expressed by the bacterial communities of the Cariaco Basin, Venezuela

© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Open Microbiology Journal 10 (2016): 140-149, doi:10.2174/1874285801610010140.Qualitative expression of dissimilative sulfite reductase (dsrA), a key gene in sulfate reduction, and sulfide:quinone oxidoreductase (sqr), a key gene in sulfide oxidation was investigated. Neither of the two could be amplified from mRNA retrieved with Niskin bottles but were amplified from mRNA retrieved by the Deep SID. The sqr and sqr-like genes retrieved from the Cariaco Basin were related to the sqr genes from a Bradyrhizobium sp., Methylomicrobium alcaliphilum, Sulfurovum sp. NBC37-1, Sulfurimonas autotrophica, Thiorhodospira sibirica and Chlorobium tepidum. The dsrA gene sequences obtained from the redoxcline of the Cariaco Basin belonged to chemoorganotrophic and chemoautotrophic sulfate and sulfur reducers belonging to the class Deltaproteobacteria (phylum Proteobacteria) and the order Clostridiales (phylum Firmicutes).Support for this work came from NSF grant MCB03-47811 to AYC, MIS, and GTT and NSF grant OCE-1061774 to VPE and CT