The Reinstatement of the Genus Kloeckeraspora Niehaus (1932) (Apiculate Yeast)

<p><strong>Abstract:</strong> The genus Kloeckeraspora Niehaus (1932) was not accepted for a long time. This paper has tried to reinstate the genus. Two phylogenetic trees were constructed for three Kloeckeraspora and four representative Hanseniaspora species. In addition, the pair-wise sequence similarities were calculated. In the phylogeneetic tree based on the 26S rRNA gene D1/D2 domain sequences derived from the neighbour-joining method, the three species of the genus Kloeckeraspora represented an extremely long branch. The calculated pair-wise sequence similarities were abnormally low (86.2 - 88.5%) between the two genera. Incidentally, the sequence similarity between Vanderwatozyma polyspora and Saccharomyces cerevisiae used as references was 94.0%. The genus Kloeckeraspora should be unequivocally accepted and retained. However, the two genera were not yet perfectly taxonomic homogeneous-natured.</p> <p><strong>Keywords:</strong> Kloeckeraspora osmophila; Kloeckerasora vineae; Kloeckeraspora occidentalis; Hanseniaspora valvyensis; Hanseniaspora uvarum; Hanseniaspora guilliermondii.</p> <p><strong>Title:</strong> The Reinstatement of the Genus Kloeckeraspora Niehaus (1932) (Apiculate Yeast)</p> <p><strong>Author:</strong> Taweesak Malimas, Huong Thi Lan Vu, Pattaraporn Yukphan, Somboon Tanasupawat, Yuzo Yamada</p> <p><strong>International Journal of Novel Research in Life Sciences                                                    </strong></p> <p><strong>ISSN 2394-966X</strong></p> <p><strong>Vol. 10, Issue 2, March 2023 - April 2023</strong></p> <p><strong>Page No: 32-37</strong></p> <p><strong>Novelty Journals</strong></p> <p><strong>Website: www.noveltyjournals.com</strong></p> <p><strong>Published Date: 03-April-2023</strong></p> <p><strong>Amendment Date: 05-October-2023</strong></p> <p><strong>DOI: <a href="https://doi.org/10.5281/zenodo.8409923">https://doi.org/10.5281/zenodo.8409923</a></strong></p> <p><strong>Paper Download Link (Source)</strong></p> <p><a href="https://www.noveltyjournals.com/upload/paper/The%20Reinstatement%20of%20the%20Genus-03042023-1.pdf"><strong>https://www.noveltyjournals.com/upload/paper/The%20Reinstatement%20of%20the%20Genus-03042023-1.pdf</strong></a></p>International Journal of Novel Research in Life Sciences, ISSN 2394-966X, Novelty Journals, Website: www.noveltyjournals.co

The generic circumscription of Mrakia and Mrakiella: The proposal of Thomashallia gen. nov.

<p><strong>Abstract:</strong> In the family Mrakiaceae, the type genus <em>Mrakia</em> senseu stricto included five teleomorphic species with the type species,<em> Mrakia frigida</em>. In contrast, the anamorphic genus <em>Mrakiella</em> sensu stricto contained nine species with the type species, <em>Mrakiella cryoconiti</em>. Between the two genera, the completely separated clusters were shown respectively in the phylogenetic tree based on the 28S rRNA gene D1/D2 domain sequences derived from the neighbour-joining method. Between <em>Mrakia frigida</em> and <em>Mrakiella</em> <em>cryoconiti</em> as well as <em>Mrakiella aquatica</em>, the pair-wise sequence similarities were 98.6% and 97.4% (1.2% width) respectively. However, the similarities between <em>Mrakia frigida</em> and the remaining four <em>Mrakia</em> species were extremely high (99.4 - 100% with 0.6% width). On the other hand, the similarity was relatively low (98.2%) between <em>Mrakiella</em> <em>cryoconiti</em> and <em>Mrakiella aquatica</em>, showing the wide range or the diversity of the anamorphic genus phylogenetically. The teleomorphic genus <em>Thomashallia</em> was newly introduced based on the formation of basidia and basidiospores with the type species, <em>Thomashallia stelviica</em>. The genus <em>Krasilnikovozyma</em> contained four species with the type species, <em>Krasilnikovozyma curviuscula</em>. Thus, the three teleomorphic genera were respectively taxonomic homogeneous-natured, and the two of the three were surely characteristic of Q-8.   </p> <p><strong>Keywords:</strong> <em>Mrakia frigida</em>; <em>Mrakiella cryoconiti</em>; <em>Thomashallia </em>gen. nov.; <em>Thomashallia stelviica</em> comb. nov.; <em>Thomashallia montana</em> comb. nov.</p> <p><strong>Title:</strong> The generic circumscription of <em>Mrakia </em>and <em>Mrakiella</em>: The proposal of <em>Thomashallia</em> gen. nov.</p> <p><strong>Author:</strong> Taweesak Malimas, Huong Thi Lan Vu, Pattaraporn Yukphan, Somboon Tanasupawat, Yuzo Yamada</p> <p><strong>International Journal of Novel Research in Life Sciences                                                    </strong></p> <p><strong>ISSN 2394-966X</strong></p> <p><strong>Vol. 10, Issue 5, September 2023 - October 2023</strong></p> <p><strong>Page No: 33-41</strong></p> <p><strong>Novelty Journals</strong></p> <p><strong>Website: www.noveltyjournals.com</strong></p> <p><strong>Published Date: 10-October-2023</strong></p> <p><strong>DOI: <a href="https://doi.org/10.5281/zenodo.8424882">https://doi.org/10.5281/zenodo.8424882</a></strong></p> <p><strong>Paper Download Link (Source)</strong></p> <p><strong><a href="https://www.noveltyjournals.com/upload/paper/The%20generic%20circumscription%20of%20Mrakia-10102023-4.pdf">https://www.noveltyjournals.com/upload/paper/The%20generic%20circumscription%20of%20Mrakia-10102023-4.pdf</a></strong></p>International Journal of Novel Research in Life Sciences, ISSN 2394-966X, Novelty Journals, Website: www.noveltyjournals.co

The Revision of Schizosaccharomycetaceae

<p><strong>Abstract:</strong> Although the genus <i>Hasegawaea </i>was introduced along with the recognition of the genus <i>Octosporomyces</i> in the classification of fission yeasts, the two additional genera were neither accepted nor recognized. However, the genus <i>Schizosaccharomyces</i> sensu Kurtzman et Robnett was taxonomically heterogeneous-natured and corresponded to a higher-ranked taxon, i.e., a monotypic family. Thus, the following three genera were confirmed in<i> </i>the family Schizosaccharomycetaceae. The genus <i>Schizosaccharomyces</i> sensu stricto was comprised of <i>Schizosacchromyces pombe,</i> the genus <i>Octosporomyces </i>was of the four species, <i>Octosporomyces octosporus</i>, <i>Octosporomyces osmophilus</i>, <i>Octosporomyces lindneri</i> and <i>Octosporomyces cryophilus</i> and the genus <i>Hasegawaea</i> was of <i>Hasegawaea japonica</i>. In conclusion, the precise classification of microorganisms will not be able to be expected in the generic designation without the presence of taxonomic homogeneous-natured taxa. The phylogenetic distances have to be absolutely considered. Namely, the longer the distances are, the more taxonomic heterogeneous-natures will be increased in the resulting genus.</p><p><strong>Keywords:</strong> <i>Schizosaccharomyces</i> sensu stricto;<i> Octosporomyces</i>; <i>Hasegawaea</i>; fission yeasts.</p><p><strong>Title:</strong> The Revision of Schizosaccharomycetaceae</p><p><strong>Author:</strong> Taweesak Malimas, Huong Thi Lan Vu, Pattaraporn Yukphan, Somboon Tanasupawat, Kozaburo Mikata, Yuzo Yamada</p><p><strong>International Journal of Novel Research in Physics Chemistry & Mathematics</strong></p><p><strong>ISSN 2394-9651</strong></p><p><strong>Vol. 10, Issue 3, September 2023 - December 2023</strong></p><p><strong>Page No: 100-106</strong></p><p><strong>Novelty Journals</strong></p><p><strong>Website: www.noveltyjournals.com</strong></p><p><strong>Published Date: 02-November-2023</strong></p><p><strong>DOI: </strong><a href="https://doi.org/10.5281/zenodo.10066158"><strong>https://doi.org/10.5281/zenodo.10066158</strong></a></p><p><strong>Paper Download Link (Source)</strong></p><p><a href="https://www.noveltyjournals.com/upload/paper/The%20Revision%20of%20Schizosaccharomycetaceae-02112023-4.pdf"><strong>https://www.noveltyjournals.com/upload/paper/The%20Revision%20of%20Schizosaccharomycetaceae-02112023-4.pdf</strong></a></p&gt

\u418\u434\u435\u43d\u442\u438\u444\u438\u43a\u430\u446\u438\u44f \u41d\u430 \u429\u430\u43c\u43e\u432\u435, \u418\u437\u43e\u43b\u438\u440\u430\u43d\u438 \u412 \u422\u430\u439\u43b\u430\u43d\u434 \u418 \u41e\u442\u43d\u435\u441\u435\u43d\u438 \u41a\u44a\u43c \u420\u43e\u434\u43e\u432\u435\u442\u435 Kozakia \u418 Swaminathania

Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora (\u201ekhem\u201d in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from a total of 181 isolated acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a water-soluble brown pigment from D-glucose on CaCO3-containing agar slants. The isolates produced acetic acid from ethanol and oxidized acetate and lactate to carbon dioxide and water, but the intensity of the acetate and lactate oxidation was weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. The isolates did not grow on 30 % D-glucose (w/v), and utilization of methanol was not found. Isolates SI15-1 and SI15-2 had peritrichous flagella and grew in the presence of either 0.35 % acetic acid (v/v) at pH 3.5, 3 % NaCl (w/v), or 1 % KNO3 (w/v). Acetate and lactate were oxidized to carbon dioxide and water, but the intensity was weak. The isolates grew on mannitol agar and glutamate agar as well as on 30 % Dglucose (w/v), but did not utilize methanol. The 16S rRNA gene sequence analysis and DNA-DNA hybridization indicated that isolates CT8-1 and CT8-2 and isolates SI15-1 and SI15-2 were unequivocally identified respectively as Kozakia baliensis and Swaminathania salitolerans .\u418\u437\u441\u43b\u435\u434\u432\u430\u43d\u438 \u441\u430 \u442\u430\u43a\u441\u43e\u43d\u43e\u43c\u438\u447\u43d\u43e \u447\u435\u442\u438\u440\u438 \u449\u430\u43c\u430, \u438\u437\u43e\u43b\u438\u440\u430\u43d\u438 \u441\u44a\u43e\u442\u432\u435\u442\u43d\u43e \u43e\u442 \u43f\u43b\u43e\u434 \u43d\u430 \u441\u430\u43f\u43e\u434\u438\u43b\u430 \u432 \u427\u430\u43d\u442\u430\u431\u443\u440\u438, \u43e\u437\u43d\u430\u447\u435\u43d\u438 \u43a\u430\u442\u43e CT8-1 \u438 CT8-2, \u438 \u43e\u442 \u441\u435\u43c\u435\u43d\u430 \u43d\u430 \u438\u43a\u441\u43e\u440\u430 (\u201e\u43a\u435\u43c\u201d \u43d\u430 \u442\u430\u439\u432\u430\u43d\u441\u43a\u438) \u432 \u420\u430\u439\u43e\u43d\u433 \u438 \u43e\u437\u43d\u430\u447\u435\u43d\u438 \u43a\u430\u442\u43e SI15-1 and SI15-2. \u427\u435- \u442\u438\u440\u438\u442\u435 \u449\u430\u43c\u430 \u441\u430 \u43f\u43e\u434\u431\u440\u430\u43d\u438 \u43e\u442 \u43e\u431\u449\u43e 181 \u438\u437\u43e\u43b\u430\u442\u430 \u43d\u430 \u43e\u446\u435\u442\u43d\u43e\u43a\u438\u441\u435\u43b\u438 \u431\u430\u43a\u442\u435\u440\u438\u438. \u429\u430\u43c\u43e\u432\u435 CT8-1 \u438 CT8-2 \u441\u430 \u43d\u435\u43f\u43e\u434\u432\u438\u436\u43d\u438 \u438 \u43f\u440\u43e\u434\u443\u446\u438\u440\u430\u442 \u43b\u435\u432\u430\u43d\u43e\u43f\u43e\u434\u43e\u431\u435\u43d \u43c\u443\u43a\u43e- \u43f\u43e\u43b\u438\u437\u430\u445\u430\u440\u438\u434 \u43e\u442 \u437\u430\u445\u430\u440\u43e\u437\u430 \u438\u43b\u438 D-\u444\u440\u443\u43a\u442\u43e\u437\u430, \u43d\u43e \u43d\u435 \u43e\u442\u434\u435\u43b\u44f\u442 \u432\u43e\u434\u43d\u43e\u440\u430\u437\u442\u432\u43e\u440\u438\u43c \u43a\u430\u444\u44f\u432 \u43f\u438\u433\u43c\u435\u43d\u442 \u43e\u442 D-\u433\u43b\u44e\u43a\u43e\u437\u430 \u432\u44a\u440\u445\u443 \u441\u43a\u43e\u441\u435\u43d \u430\u433\u430\u440, \u441\u44a\u434\u44a\u440\u436\u430\u449 CaCO3. \u418\u437\u43e\u43b\u430- \u442\u438\u442\u435 \u43f\u440\u43e\u438\u437\u432\u435\u436\u434\u430\u442 \u43e\u446\u435\u442\u43d\u430 \u43a\u438\u441\u435\u43b\u438\u43d\u430 \u43e\u442 \u435\u442\u430\u43d\u43e\u43b \u438 \u43e\u43a\u438\u441\u43b\u44f\u432\u430\u442 \u430\u446\u435\u442\u430\u442 \u438 \u43b\u430\u43a\u442\u430\u442 \u434\u43e \u432\u44a\u433\u43b\u435\u440\u43e\u434\u435\u43d \u434\u432\u443\u43e\u43a\u438\u441 \u438 \u432\u43e\u434\u430, \u43d\u43e \u438\u43d\u442\u435\u43d\u437\u438\u432\u43d\u43e\u441\u442\u442\u430 \u43d\u430 \u43e\u43a\u438\u441\u43b\u435\u43d\u438\u435\u442\u43e \u435 \u441\u43b\u430\u431\u430. \u422\u435\u445\u43d\u438\u44f\u442 \u440\u430\u441\u442\u435\u436 \u43d\u435 \u441\u435 \u438\u43d\u445\u438\u431\u438\u440\u430 \u43e\u442 0.35 % \u43e\u446\u435\u442\u43d\u430 \u43a\u438\u441\u435\u43b\u438\u43d\u430 (v/v) \u43f\u440\u438 pH 3.5. \u418\u437\u43e\u43b\u430\u442\u438\u442\u435 \u43d\u435 \u440\u430\u441\u442\u430\u442 \u432\u44a\u440\u445\u443 30 % D-\u433\u43b\u44e\u43a\u43e\u437\u430 (w/v), \u430 \u443\u441\u432\u43e\u44f\u432\u430\u43d\u435 \u43d\u430 \u43c\u435\u442\u430\u43d\u43e\u43b \u43d\u435 \u441\u435 \u443\u441\u442\u430\u43d\u43e\u432\u44f\u432\u430. \u429\u430\u43c\u43e\u432\u435 SI15-1 \u438 SI15-2 \u438\u43c\u430\u442 \u43f\u435\u440\u438\u442\u440\u438\u445\u430\u43b\u43d\u43e \u440\u430\u437\u43f\u43e\u43b\u43e\u436\u435\u43d\u438 \u440\u435\u441\u43d\u438 \u438 \u440\u430\u441\u442\u430\u442 \u432 \u43f\u440\u438\u441\u44a\u441\u442\u432\u438\u435 \u43a\u430\u43a\u442\u43e \u43d\u430 0.35 % \u43e\u446\u435\u442\u43d\u430 \u43a\u438\u441\u435\u43b\u438\u43d\u430 (v/v) \u43f\u440\u438 pH 3.5, \u442\u430\u43a\u430 \u438 \u43d\u430 3 % NaCl (w/v) \u438\u43b\u438 1 % KNO3 (w/v). \u410\u446\u435\u442\u430\u442\u44a\u442 \u438 \u43b\u430\u43a\u442\u430\u442\u44a\u442 \u441\u435 \u43e\u43a\u438\u441\u43b\u44f\u432\u430\u442 \u434\u43e \u432\u44a\u433\u43b\u435\u440\u43e\u434\u435\u43d \u434\u432\u443\u43e\u43a\u438\u441 \u438 \u432\u43e\u434\u430, \u43d\u43e \u438\u43d\u442\u435\u43d\u437\u438\u432\u43d\u43e\u441\u442\u442\u430 \u435 \u441\u43b\u430\u431\u430. \u418\u437\u43e\u43b\u430\u442\u438\u442\u435 \u440\u430\u441\u442\u430\u442 \u432\u44a\u440\u445\u443 \u430\u433\u430\u440 \u441 \u43c\u430\u43d\u438\u442\u43e\u43b \u438\u43b\u438 \u433\u43b\u443\u442\u430\u43c\u430\u442, \u43a\u430\u43a\u442\u43e \u438 \u432\u44a\u440\u445\u443 30 % D-\u433\u43b\u44e\u43a\u43e\u437\u430 (w/v), \u43d\u43e \u43d\u435 \u443\u441\u432\u43e\u44f\u432\u430\u442 \u43c\u435\u442\u430\u43d\u43e\u43b. \u41f\u440\u43e\u432\u435\u434\u435\u43d\u438\u442\u435 \u441\u435\u43a\u432\u435\u43d- \u446\u438\u43e\u43d\u435\u43d \u430\u43d\u430\u43b\u438\u437 \u43d\u430 16S \u440\u420\u41d\u41a \u438 \u414\u41d\u41a-\u414\u41d\u41a \u445\u438\u431\u440\u438\u434\u438\u437\u430\u446\u438\u44f \u43f\u43e\u43a\u430\u437\u432\u430\u442, \u447\u435 \u449\u430\u43c\u43e\u432\u435 CT8-1 and CT8-2 \u431\u435\u437 \u441\u44a\u43c\u43d\u435\u43d\u438\u435 \u441\u435 \u438\u434\u435\u43d\u442\u438\u444\u438\u446\u438\u440\u430\u442 \u43a\u430\u442\u43e Kozakia baliensis , \u430 \u438\u437\u43e\u43b\u430\u442\u438 SI15-1 \u438 SI15-2 \u43a\u430\u442\u43e Swaminathania salitolerans

Идентификация На Щамове, Изолирани В Тайланд И Отнесени Към Родовете Kozakia И Swaminathania

Four isolates, isolated from fruit of sapodilla collected at Chantaburi and designated as CT8-1 and CT8-2, and isolated from seeds of ixora („khem” in Thai, Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were examined taxonomically. The four isolates were selected from a total of 181 isolated acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a water-soluble brown pigment from D-glucose on CaCO3-containing agar slants. The isolates produced acetic acid from ethanol and oxidized acetate and lactate to carbon dioxide and water, but the intensity of the acetate and lactate oxidation was weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. The isolates did not grow on 30 % D-glucose (w/v), and utilization of methanol was not found. Isolates SI15-1 and SI15-2 had peritrichous flagella and grew in the presence of either 0.35 % acetic acid (v/v) at pH 3.5, 3 % NaCl (w/v), or 1 % KNO3 (w/v). Acetate and lactate were oxidized to carbon dioxide and water, but the intensity was weak. The isolates grew on mannitol agar and glutamate agar as well as on 30 % Dglucose (w/v), but did not utilize methanol. The 16S rRNA gene sequence analysis and DNA-DNA hybridization indicated that isolates CT8-1 and CT8-2 and isolates SI15-1 and SI15-2 were unequivocally identified respectively as Kozakia baliensis and Swaminathania salitolerans .Изследвани са таксономично четири щама, изолирани съответно от плод на саподила в Чантабури, означени като CT8-1 и CT8-2, и от семена на иксора („кем” на тайвански) в Районг и означени като SI15-1 and SI15-2. Че- тирите щама са подбрани от общо 181 изолата на оцетнокисели бактерии. Щамове CT8-1 и CT8-2 са неподвижни и продуцират леваноподобен муко- полизахарид от захароза или D-фруктоза, но не отделят водноразтворим кафяв пигмент от D-глюкоза върху скосен агар, съдържащ CaCO3. Изола- тите произвеждат оцетна киселина от етанол и окисляват ацетат и лактат до въглероден двуокис и вода, но интензивността на окислението е слаба. Техният растеж не се инхибира от 0.35 % оцетна киселина (v/v) при pH 3.5. Изолатите не растат върху 30 % D-глюкоза (w/v), а усвояване на метанол не се установява. Щамове SI15-1 и SI15-2 имат перитрихално разположени ресни и растат в присъствие както на 0.35 % оцетна киселина (v/v) при pH 3.5, така и на 3 % NaCl (w/v) или 1 % KNO3 (w/v). Ацетатът и лактатът се окисляват до въглероден двуокис и вода, но интензивността е слаба. Изолатите растат върху агар с манитол или глутамат, както и върху 30 % D-глюкоза (w/v), но не усвояват метанол. Проведените секвен- ционен анализ на 16S рРНК и ДНК-ДНК хибридизация показват, че щамове CT8-1 and CT8-2 без съмнение се идентифицират като Kozakia baliensis , а изолати SI15-1 и SI15-2 като Swaminathania salitolerans