Efeito do óleo essencial de Piper hispidinervum sobre Moniliophthora perniciosa.

O objetivo deste trabalho foi avaliar o efeito do óleo essencial de Piper hispidinervum sobre a germinação de esporos e do crescimento micelial de M. perniciosa in vitro.Resumo:94-1

Comparative parasitism of the fish plagioscion squamosissimus in native and invated river basins.

Biological invasions are considered a major threat to biodiversity around the world, but the role of parasites in this process is still little investigated. Here, we compared parasite infections of a host species in the areas where it originated and where it was introduced, and in native and introduced species in the same environment, using the endoparasites of the fish Plagioscion squamosissimus (Sciaenidae) in 3 Brazilian basins. Samples were taken in 2 rivers where the species is native, i.e., Solimões River (SO) and Tocantins River (TO), and where the species was introduced, the upper Parana´ River (PR). In addition, abundances of diplostomids and larval nematodes were compared between P. squamosissimus and 2 native competitors in the PR, Hoplias malabaricus and Raphiodon vulpinus. In total, 13 species of endoparasites were recorded, but only Austrodiplostomum sp. and cestode cysts were present in all localities. Although infracommunity richness was similar, their species composition was slightly different among localities. General linear models using the relative condition factor of fish as response variables, and abundance of the most prevalent parasites as possible predictors showed that the condition of fish is negatively correlated with parasite abundance only in the native range (TO). Abundance of diplostomid eye flukes was higher in the PR, and in the native species H. malabaricus when compared to the invader, which might present an advantage for P. squamosissimus if they compete for prey. However, although P. squamosissimus may have lost some of its native parasites during its introduction to the PR, it is now possibly acting as a host for native generalist parasites

Avaliação de fungicidas para o controle da ferrugem da videira.

Suplemento, ref. 257. Edição dos Resumos do XXXV Congresso Paulista de Fitopatologia, Jaguariúna, fev. 2012

Fontes de resistência de videira ao fungo oídio no Nordeste brasileiro.

Foram avaliadas 135 variedades distintas em três ciclos, observando-se as mesmas plantas sem nenhum oídicida

Supported ionic liquid materials for L-asparaginase immobilization

L-asparaginase (ASNase) (L-asparagine amidohydrolase EC 3.5.1.1) has been widely used as a therapeutic agent in the treatment of acute lymphoblastic leukemia (ALL) and in the food industry for the removal of toxic acrylamide (formed in foods cooked at high temperatures). Accordingly, ASNase is also used in biosensors for leukemia diagnosis. To improve the performance of ASNase and overcome the limitations of free enzymes, namely low stability and biocatalytic activity, enzyme immobilization is one of the most used strategies. Several supports as carbon nanotubes, graphene and chitosan have been reported for ASNase immobilization. Among them, nanomaterials, and in particular silica, have emerged as a promising alternative support for enzyme immobilization due to their unique characteristics, such as biological compatibility and high surface to volume ratio, being thus identified as promising supports for ASNase. In this work, supported ionic liquid materials (SILs) based on silica were used as novel immobilization supports for ASNase by a simple adsorption method. Different experimental conditions, namely contact time, medium pH and ASNase/SILs ratio were evaluated. The performance of the immobilized enzyme was studied by measuring its activity through the monitoring of the hydrolysis of the substrate, Lasparagine. Characterization of the ASNase-SILs bioconjugate was carried out to evaluate the adsorption of the ASNase onto the supports. The immobilization of ASNase onto the SILs was successfully achieved with an activity of immobilized ASNase ranging from 0.6 to 0.86 U of enzyme per mg of SILs under the optimum immobilization conditions (60 min, pH 8.0 and 0.06 mg.mL-1 of ASNase in 10 mg of SILs).publishe

Static quantities of a neutral bilepton in the 331 model with right-handed neutrinos

A neutral vector boson can possess static electromagnetic properties provided that the associated field is no self-conjugate. This possibility is explored in the $SU_C(3) X SU_L(3) X U_N(1)$ model with right-handed neutrinos, which predicts a complex neutral gauge boson $Y^0$ in a nontrivial representation of the electroweak group. In this model the only nonvanishing form factors are the CP-even ones, which arise from both the quark and gauge sectors, and contribute to the magnetic dipole and the electric quadrupole moments of this neutral particle.Comment: 10 pages, 6 figures, submitted to Phys. Rev.

In vitro efficacy of emamectin benzoate and glyphosate against monogenean parasites from Colossoma macropomum.

Este estudo investigou a eficácia in vitro do benzoato de emamectina e do glifosato contra monogenéticos de Colossoma macropomum e a tolerância dessa espécie a essas drogas terapêuticas. Ensaios in vitro demonstraram que as concentrações de benzoato de emamectina (12,5; 16,6; 20,8 e 25,0g/L) e de glifosato (2.000; 3.000; 4.000 e 5.000mg/L) foram 100% eficazes contra Anacanthorus spathulatus, Notozothecium janauachensis e Mymarothecium boegeri. As maiores concentrações de benzoato de emamectina (20,8 e 25,0g/L) foram 100% efetivas após 30 minutos de exposição. Para o glifosato, as maiores concentrações foram as mais efetivas, sendo que 4.000 e 5.000 mg/L imobilizaram 100% dos parasitos em 45 minutos e 30 minutos, respectivamente. A microscopia eletrônica de varredura revelou que os parasitos expostos a 20,8 e 25,0g/L de benzoato de emamectina e 5.000 mg/L de glifosato apresentaram tegumento coberto por rugas mais profundas. Alterações comportamentais ocorreram em C. macropomum exposto a todas as concentrações de ambas as drogas, como mortalidade após exposição a benzoato de emamectina e glifosato a 2,0;12,5 e 25,0g/L e 500; 1.000; 2.000; 3.000; 4.000 e 5.000mg/L, respectivamente. Baixas concentrações de benzoato de emamectina e de glifosato foram toleradas por C. macropomum, mas essas baixas concentrações podem não ter eficácia contra infecções por monogenéticos

Low-cost class a evapotranspiration pan for reference evapotranspiration estimation in protected and field environments: a practical approach.

Currently, there are various methods for determining the Class A pan coefficient (Kp) in estimating reference evapotranspiration (ETo), which is of utmost importance for water management in agriculture. This study aimed to estimate ETo inside and outside agricultural greenhouses using a low-cost constructed Class A pan (TCA-c) method. To estimate ETo, it is necessary to establish the Kp, as ETo is the product of ECA multiplied by Kp. This study employed the calibration method, comparing ETo determined by Penman-Monteith (EToPM) with Class A pan evaporation (ECA) to determine Kp. EToPM served as the standard for correlations with reference evapotranspiration using the TCA-c method inside and outside an agricultural greenhouse, avoiding the installation of a "Class A" pan inside the greenhouse. The experiment was conducted at UFF's Gragoatá campus in Niterói - RJ. Four TCA-c pans were installed (three inside and one outside an agricultural greenhouse) and managed for one year. Principal Component Analyses (PCA) revealed significant differences in ETo throughout the seasons. Adjusted Kps were established for all pans. It was observed that ETo inside the greenhouse was lower than that estimated outside. It is recommended to install the TCA-c pan inside the greenhouse for ETo estimation, utilizing different Kps throughout the seasons

Out-of-phase oscillation between superfluid and thermal components for a trapped Bose condensate under oscillatory excitation

The vortex nucleation and the emergence of quantum turbulence induced by oscillating magnetic fields, introduced by Henn E A L, et al. 2009 (Phys. Rev. A 79, 043619) and Henn E A L, et al. 2009 (Phys. Rev. Lett. 103, 045301), left a few open questions concerning the basic mechanisms causing those interesting phenomena. Here, we report the experimental observation of the slosh dynamics of a magnetically trapped $^{87}$Rb Bose-Einstein condensate (BEC) under the influence of a time-varying magnetic field. We observed a clear relative displacement in between the condensed and the thermal fraction center-of-mass. We have identified this relative counter move as an out-of-phase oscillation mode, which is able to produce ripples on the condensed/thermal fractions interface. The out-of-phase mode can be included as a possible mechanism involved in the vortex nucleation and further evolution when excited by time dependent magnetic fields.Comment: 5 pages, 5 figures, 25 reference

Reusability of L-asparaginase immobilized on silica-based supported ionic liquids

L-asparaginase (ASNase) is an aminohydrolase enzyme used as an anticancer drug, e.g. in the treatment of acute lymphoblastic leukemia, in acrylamide reduction and in biosensing. Nevertheless, its low stability and thermolability, and susceptibility to proteases, hinder its application in the health and food industries. Hence, the improvement of its properties through efficient immobilization methods is in high demand. Thus, this work aims the development of silica-based supported ionic liquids (SILs) for the ASNase immobilization to improve its stability and enable its reusability. While activated silica with no ILs only kept total initial ASNase activity during the first cycle of reaction, SILs allowed 5 cycles of reaction, keeping 82% of initial ASNase activity, reinforcing their potential as alternative enzymatic supports.publishe