Human Fetal Tissue: Scientific Uses and Ethical Concerns

Human fetal tissue has been used in research for decades, but recent attempts to implant fetal neural tissue as therapy for Parkinson\u27s disease have stimulated discussion of ethical and policy issues. In late 1989 a moratorium on federal support of fetal tissue transplantation research was indefinitely extended, based on the connection between this research and elective abortion. Four abortion-related objections to the use of fetal tissue can be identified: 1. The procedures of abortion and tissue procurement are linked in practice; 2. One who uses fetal tissue is complicit with the abortions which provided the tissue; 3. The prospect of therapeutic use of tissue could influence some women to choose abortion; 4. The therapeutic success of fetal tissue transplants could lead to greater public acceptance of elective abortion. The moral significance of these objections is currently being debated

In Vitro Fertilization: Are There Still Ethical Problems?

The initial development of the technique of in vitro fertilization (IVF) was accompanied by discussion of related ethical questions. Two significant ones were: Did the development of IVF justify the wastage of human embryos which made it possible? Was it justifiable to attempt human embryo transfer without laboratory studies to determine the safety of the technique? Thousands of births as a result of IVF have largely made these two questions moot. But four others remain: Is IVF immoral because it accomplishes procreation in a laboratory rather than through an act of marital union? Is it permissible to maximize the success of IVF through practices which result in the intentional destruction of human embryos or fetuses? Is it ethical to conduct basic scientific research which utilizes laboratory-fertilized embryos? Given uneven and often very low success rates for IVF, should public policy act to protect clients or to promote improved IVF practice? While our society is deeply divided as to the resolution of these problems, it is beginning to engage in public debate, particularly on the last two questions

Swelling of latex particles—towards a solution of the riddle

The assumption that during emulsion polymerization, the monomer molecules simply diffuse through the aqueous phase into the latex particles is a commonplace. However, there are experimental hints that this might not be that easy. Here, simulation results are discussed based on Fick’s diffusion laws regarding the swelling of latex particles. The results of quantitative application of these laws for swelling of latex particles allow the conclusion that the instantaneous replenishment of the consumed monomer during emulsion polymerization requires a close contact between the monomer and the polymer particles

Towards a consistent mechanism of emulsion polymerization—new experimental details

The application of atypical experimental methods such as conductivity measurements, optical microscopy, and nonstirred polymerizations to investigations of the ‘classical’ batch ab initio emulsion polymerization of styrene revealed astonishing facts. The most important result is the discovery of spontaneous emulsification leading to monomer droplets even in the quiescent styrene in water system. These monomer droplets with a size between a few and some hundreds of nanometers, which are formed by spontaneous emulsification as soon as styrene and water are brought into contact, have a strong influence on the particle nucleation, the particle morphology, and the swelling of the particles. Experimental results confirm that micelles of low-molecular-weight surfactants are not a major locus of particle nucleation. Brownian dynamics simulations show that the capture of matter by the particles strongly depends on the polymer volume fraction and the size of the captured species (primary free radicals, oligomers, single monomer molecules, or clusters)

The Complexity of Packing Edge-Disjoint Paths

We introduce and study the complexity of Path Packing. Given a graph G and a list of paths, the task is to embed the paths edge-disjoint in G. This generalizes the well known Hamiltonian-Path problem. Since Hamiltonian Path is efficiently solvable for graphs of small treewidth, we study how this result translates to the much more general Path Packing. On the positive side, we give an FPT-algorithm on trees for the number of paths as parameter. Further, we give an XP-algorithm with the combined parameters maximal degree, number of connected components and number of nodes of degree at least three. Surprisingly the latter is an almost tight result by runtime and parameterization. We show an ETH lower bound almost matching our runtime. Moreover, if two of the three values are constant and one is unbounded the problem becomes NP-hard. Further, we study restrictions to the given list of paths. On the positive side, we present an FPT-algorithm parameterized by the sum of the lengths of the paths. Packing paths of length two is polynomial time solvable, while packing paths of length three is NP-hard. Finally, even the spacial case Exact Path Packing where the paths have to cover every edge in G exactly once is already NP-hard for two paths on 4-regular graphs

Polyamide capsules via soft templating with oil drops—1. Morphological studies of the capsule wall

Poly(terephthalamide) microcapsules can be reproducibly and easily prepared by interfacial polycondensation around emulsion droplets in water. Oil drops of cyclohexane/chloroform mixture stabilized with poly(vinyl alcohol) containing terephthaloylchloride serve as soft template. The interfacial polycondensation starts immediately after addition of an amine mixture (hexamethylenediamine/diethylenetriamine). Light and scanning electron microscopy prove the formation of capsules with size distribution in the range from a few up to 100 µm depending on particular composition of the reaction mixture. The morphology of the capsule wall is characterized by precipitated particles. If instead of pure organic solvents a reactive oil phase is used as template, the capsules can serve in subsequent reactions as templates for the synthesis of composite particles. In this way, styrene can be radically polymerized inside the capsule leading to composite capsules. The capsule morphology is determined by the partition of all components between all phases

Studying Side Effects of Tyrosine Kinase Inhibitors in a Juvenile Rat Model with Focus on Skeletal Remodeling

The tyrosine kinase (TK) inhibitor (TKI) imatinib provides a highly effective treatment for chronic myeloid leukemia (CML) targeting at the causative oncogenic TK BCR-ABL1. However, imatinib exerts off-target effects by inhibiting other TKs that are involved, e.g., in bone metabolism. Clinically, CML patients on imatinib exhibit altered bone metabolism as a side effect, which translates into linear growth failure in pediatric patients. As TKI treatment might be necessary for the whole life, long-term side effects exerted on bone and other developing organs in children are of major concern and not yet studied systematically. Here, we describe a new juvenile rat model to face this challenge. The established model mimics perfectly long-term side effects of TKI exposure on the growing bone in a developmental stage-dependent fashion. Thus, longitudinal growth impairment observed clinically in children could be unequivocally modeled and confirmed. In a “bench-to-bedside” manner, we also demonstrate that this juvenile animal model predicts side effects of newer treatment strategies by second generation TKIs or modified treatment schedules (continuous vs. intermittent treatment) to minimize side effects. We conclude that the results generated by this juvenile animal model can be directly used in the clinic to optimize treatment algorithms in pediatric patients