Infection of Chicken Anaemia Virus: Etiology, Epidemiology, Clinical Sign, Pathological Changes and Disease Control

Initially, Chicken anaemia virus (CAV) is known as CAA, which was first isolated by Yuasa in Japan in 1976. CAV particles are non enveloped with a diameter of 19 .1-20 .7nm, belonging to the family Circoviridae, genus Gyrovirus . CAV infection was first appeared in Indonesia at the same time as the outbreak of stunting and runting syndrome in 1996 with a mortality rate of 5-15% but it may reach to 60%. CAV can be transmitted vertically or horizontally. Chicken all ages is susceptible to infection. Infection of CAV occurred in young chicken flock at 2-3 weeks growth of age, causing clinical signs while in old chicken flock which is sub clinical. The signs of infectious of CAV are retarded, anaemia, anorexia, pale of face and pial. The pathology anatomy changes are pale carcases, yellowish bone marrow, atrophy of thymus and bursa fabricius . Whereas, histophatological changes are thymic necrosis of cortex and medulla, lymphocyte depletion of thymus, bursa fabricius and bone marrow. Diagnose of CAV is based on pathological changes and followed by the isolation of certain lymphoblastoid chicken cell MDCC-MSB I and then, is identified by virus neutralization. The presence of virus can also be identified by immunofluorescent and immunoperoxidase staining, in situ hybridization technique or PCR. For antibody detection, ELISA technique can be used. The syndromes of CAV infection are closely associated with those of osteopetrosis, reovirus, infectious bursal disease (IBD) and Marek. Vaccination programme in breeding farm is needed to induce maternal antibody. This paper describes the CAV disease and its occurrence in Indonesia. Key word : Chicken anaemia virus, diagnose, pathology anatomy, histopathology, contro

Seroepidemiology of Canine parvovirus infection in dogs

Canine parvovirus is an acute and fatal viral disease in dogs. A total of 209 local, cross breed and breed dogs sera from Kodya Bogor, Kabupaten Bogor, Sukabumi, and Jakarta, had been tested using Haemagglutination Inhibition Test (HI) with pig red blood cells. A total of 64 breed and cross breed dogs from Sukabumi and Kodya Bogor, were used as a sentinel dogs to study the epidemiology of Canine parvovirus (CPV) infection and its immunological responses caused by vaccination. The results indicated that 78% (95) breed and cross bred dogs and 59% (51) local dogs had antibody to CPV. Sentinel dogs results indicated that dogs had been vaccinated showed antibody response with the varied titre dependant upon prevaccination titre. Low prevaccinated titre gave better response than protective level titre. From 19 puppies observed, Maternal antibodi were still detected until 5 weeks old puppies. First vaccination given at less than 3 months old, should be boosted after 3 months old puppied. Antibodi titre produced by natural infection will keep untill 2 years. These data concluded that the dog condition and time of vaccination will affect the optimum antibody response.   Key words: Canine parvovirus, haemagglutination inhibition, isolatio

Ragam Jenis Nyamuk di Sekitar Kandang Babi dan Kaitannya dalam Penyebaran Japanese Encephalitis

The mosquitoes species in pig pen area and its relation to the trasmission of Japanese Encephalitis(JE) in North Sumatra Province was studied as the first step of the investigation on JE prevalence inIndonesia. The aim of this research is to determined the mosquitoes species that can be a potential vectorsof JE and its relation to the prevalence of JE reactor of pigs in North Sumatra. Mosquito collections werecarried out by using ultra violete light trap, Magoon trap and human landing collections. Serological studywas done by using competitive enzyme linked-immunosorbent assay (C-ELISA) methode. The resultsshowed several mosquitoes species i.e. Culex tritaeniorhynchus, Cx. quinquefasciatus, and Cx. fuscocephalusthat can be a potential vectors of JE was found in this province. The environmental conditions and the pigfarm and horses stable management supported the development of ideal breeding places for mosquitoesand other blood sucking flies. It was reported also that the prevalence of JE reactors in pig farms and horsestable in North Sumatra was high (71.67%). The high prevalence of JE reactors indicated that the infectionof JE was actively occured between pigs-mosquitoes-pigs in thise area

Epidemiology of Japanese–B– encephalitis infection in pigs in Riau and North Sumatera Provinces

Epidemiology study on Japanese-B-Encephalitis (JE) was conducted in Riau and North Sumatera Provinces. A total of 190 pig sera from Riau Province and 164 pig sera from North Sumatera were tested using competitive ELISA (C-ELISA) to detect antibodies against JE virus. Insect collection was also conducted using several methods near pig farms in those provinces and identified into species to gain more information on its role to distribute JE infection. Serological results indicated that 70% pig in Sumatera and 94% pig in Riau had antibodies against JE virus. The highest prevalence of reaktor was detected in pig of more than 4 months age in both Provinces. The results of insect collection showed that Culex tritaeniorchynchus and Culex quinquefasciatus were the most dominant species in both provinces. Based on serological testing, indicated that JE virus infected pig in Sumatera and Riau Provinces, and higher reactor was obtained in older pig. Culex tritaeniorchynchus and Culex quinquefasciatus were the dominant insect species in both provinces, hence those species had a possibility to play an important role of JE transmission.   Key words: JE, pigs, serology, insect

Seroepidemiologi Nipah Virus Pada Kalong Dan Ternak Babi Di Beberapa Wilayah Di Indonesia

Nipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia

Developing Farm-Level Post-vaccination Sero-Monitoring Systems for H5N1 Highly Pathogenic Avian Influenza in an Endemically Infected Country

Whilst the serological responses of poultry following vaccination against highly pathogenic avian influenza H5N1 has been extensively investigated under laboratory conditions, there have been fewer studies conducted in the field. This applies particularly to the endemically infected countries routinely practicing vaccination, where the combination of multiple circulating clades and/or the use of vaccines with different seed strains makes the design and interpretation of field studies especially problematic. To address this for the particular situation of layer hens in the small to medium commercial sector in Indonesia, we developed a sampling regime before and after the vaccination given to point-of-lay pullets, and assessed serological response with a panel of test antigens. This confirmed that high titres were induced in those birds vaccinated with locally produced homologous H5N1 vaccines administered two or more times, but in flocks using imported heterologous H5N2 vaccines median titres were significantly lower, and unlikely to provide protection throughout the production cycle, without additional vaccination. Comparing the HI responses against the panel of antigens enabled the detection of the flock's exposure to different vaccine antigens, and made possible the detection of mislabelled vaccine seed strains. Furthermore, we show that test antigens need not be exactly matched to assess sero-protection in well vaccinated birds. Finally our study suggests that the POL vaccination serves as a useful reference point for following cohorts of layers throughout their production cycle, and thus enabling robust vaccination field effectiveness studies

Gambaran seroepidemiologi dan histopatologi infeksi virus parainfluenza tipe 3 pada sapi

A study to gain seroepidemiological feature and histopathological changes in order to obtain a viral causative agent had been conducted against parainfluenza virus type 3 (PI-3) in infection cattle in Indonesia. Serological survey was conducted in different areas in Indonesia and from serum Bank to gain the information on the distribution of parainfluenza type 3 (PI-3) in large ruminants. A total of 1334 sera had been tested using serum neutralization test, and the result indicated that prevalence of reactors was varied from 0 to 60 %. The highest prevalence was 60% in sera detected from Bogor abbatoir. Reactors were also found in other areas such as West Java, Central Java, East Java, NTT and Papua. Titration results indicated that the distribution of titre was varied from 4 to 256, and titre of 8 to 32 was the most common. Titre of 128 and 256 was only found in each of 1 sera only. Isolation results indicated that no isolate was obtained from 237 samples processed. Histological examination showed that more than 60% had interstitial pneumonia, which indicated vairal infection had been occurred. This serological result indicated that PI-3 infection was detected in Indonesian large ruminants

Gambaran seroepidemiologi dan histopatologi infeksi virus parainfluenza tipe 3 pada sapi

A study to gain seroepidemiological feature and histopathological changes in order to obtain a viral causative agent had been conducted against parainfluenza virus type 3 (PI-3) in infection cattle in Indonesia. Serological survey was conducted in different areas in Indonesia and from serum Bank to gain the information on the distribution of parainfluenza type 3 (PI-3) in large ruminants. A total of 1334 sera had been tested using serum neutralization test, and the result indicated that prevalence of reactors was varied from 0 to 60 %. The highest prevalence was 60% in sera detected from Bogor abbatoir. Reactors were also found in other areas such as West Java, Central Java, East Java, NTT and Papua. Titration results indicated that the distribution of titre was varied from 4 to 256, and titre of 8 to 32 was the most common. Titre of 128 and 256 was only found in each of 1 sera only. Isolation results indicated that no isolate was obtained from 237 samples processed. Histological examination showed that more than 60% had interstitial pneumonia, which indicated vairal infection had been occurred. This serological result indicated that PI-3 infection was detected in Indonesian large ruminants.   Key words: Parainfluenza type 3 virus, serology, histopatholog

Serological study against transmissible gastroenteritis (TGE) virus in several area in Indonesia

A number of 1,168 pig and dog sera from 8 provinces in Indonesia were tested serologically for transmissible gastroenteritis (TGE) antibodies using serum neutralisation test to detect the prevalence of TGE in Indonesia. The sera were obtained from serum bank at Research Institute for Veterinary Science, Bogor. All sera collected before 1995 were negative antibody to TGE. However, sera collected from 2 provinces Sumatera Utara and Sulawesi Utara in 1996 had antibodies against TGE virus (14.03%). Titration of reacted sera showed varied between titres of 8 to 128.   Key words: Transmissible gastroenteritis virus, serum neutralization tes

Infection of Parainfluenza type 3 (PI-3) as one of the causative agent of pneumonia in sheep and goats

Serological survey was conducted to obtain the prevalence of Parainfluenza type 3 (PI-3) reactor as one of the causative agent of pneumonia in sheep and goats in abatoir at Jakarta and some small holder farms in Indonesia. Serological test using serum neutralization from 724 goat sera and 109 sheep sera indicated that only 1% of goats were serologically reactors and none of sheep sera had antibodies against PI-3 virus. Isolation of the virus from 56 bronchus and trachea swab and 345 lungs indicated that only one sampel from lung showed cythopathic effect (CPE) in Madin Darby Bovine Kidney (MDBK) cell lines identification of the virus using serum neutralization test indicated that the virus neutralized reference PI-3 antisera. The isolate came from one lung (7%) of 24 that showed histopathologically pneumonia intertitialis that usually caused by viral infection