HPLC analysis of technetium(I)-99m labelled C60(OH)22

Fullerenols, water-soluble polihydroxylated fullerenes, are very important kinds of fullerene derivatives because it is suitable for biological study. In order to get convenient substance for studies (in vivo and in vitro) we investigate possibilities of labeling fullerenol. The HPLC results performed by isocratic HPLC, confirmed that hydrophilic organometallic [99mTc(CO)3(H2O)3]+ precursor allows forming of 99mTc(I) complexes with fullerenol.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

The influence of heparin on the biological distribution of 99mTc-glucoheptonate

Ispitan je uticaj antikoagulantnog leka heparina na radiohemijski sastav i biološku distribuciju renalnog preparata 99mTc-glukoheptonata. Međudejstvo leka i radiofarmaceutika ispitano je u invitro uslovima, primenom radiohromatografske metode. Povećana koncentracija heparina dala je smanjenje frakcije 99mTc-glukoheptonata i povećan sadržaj frakcije koja odgovara 99mTc-heparinu. Biološkim eksperimentima potvrđeno je medudejstvo leka i radiofarmaceutika u in vivo uslovima, što se manifestovalo u smanjenju lokalizacije 99mTc-glukoheptonata u bubrezima.The influence of heparin as the anticoagulant drug on the radiochemical composition and biological distribution of the renal reagent 99mTc-Glucoheptonate was examined. The interaction of the drug and radiopharmaceutical was examined under »in vitro« conditions, by radiochromatographic method. The increased concentration of heparin caused the decrease of 99mTc-Glucoheptonate fracton and, increased content of 99mTc-heparin fraction. Biological experiment confirmed interaction of drug and radiopharmaceutical also in »in vivo« conditions. Decreased localization of 99mTc-Glucoheptonate in kidneys was observed

The influence of heparin on the biological distribution of 99mTc-glucoheptonate

Ispitan je uticaj antikoagulantnog leka heparina na radiohemijski sastav i biološku distribuciju renalnog preparata 99mTc-glukoheptonata. Međudejstvo leka i radiofarmaceutika ispitano je u invitro uslovima, primenom radiohromatografske metode. Povećana koncentracija heparina dala je smanjenje frakcije 99mTc-glukoheptonata i povećan sadržaj frakcije koja odgovara 99mTc-heparinu. Biološkim eksperimentima potvrđeno je medudejstvo leka i radiofarmaceutika u in vivo uslovima, što se manifestovalo u smanjenju lokalizacije 99mTc-glukoheptonata u bubrezima.The influence of heparin as the anticoagulant drug on the radiochemical composition and biological distribution of the renal reagent 99mTc-Glucoheptonate was examined. The interaction of the drug and radiopharmaceutical was examined under »in vitro« conditions, by radiochromatographic method. The increased concentration of heparin caused the decrease of 99mTc-Glucoheptonate fracton and, increased content of 99mTc-heparin fraction. Biological experiment confirmed interaction of drug and radiopharmaceutical also in »in vivo« conditions. Decreased localization of 99mTc-Glucoheptonate in kidneys was observed

Study of corrosion of aluminium alloys of nuclear purity in ordinary water, пart one

Effects of corrosion of aluminum alloys of nuclear purity in ordinary water of the spent fuel storage pool of the RA research reactor at VINČA Institute of Nuclear Sciences has been examined in the frame work of the International Atomic Energy Agency Coordinated Research Project "Corrosion of Research Reactor Aluminum-Clad Spent Fuel in Water" since 2002. The study presented in this paper comprises activities on determination and monitoring of chemical parameters and radio activity of water and sludge in the RA spent fuel storage pool and results of the initial study of corrosion effects obtained by visual examinations of surfaces of various coupons made of aluminum alloys of nuclear purity of the test racks exposed to the pool water for a period from six months to six years

Radiochemical purity and particles number determinations of modified Tc-99m-macroaggregated albumin

A new procedure for the aggregation of human albumin and Tc-99m-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in Tc-99m-MAA were tested. Thus, 85% methanol was used as the mobile phase in paper and ITL chromatography with Whatman N-o 1 and ITLC-SA strips. A system of two solvents (acetone and 1 M NaCl or 0.9% NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Filtration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency ( GT 98.5%) and percent radiochemical impurities of Tc-99m-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000-350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes

Particle size analysis: (90)Y and (99m)Tc-labelled colloids

Colloidal particle size is an important characteristic to consider when choosing a radiopharmaceutical for diagnosis and therapeutic purposes in nuclear medicine. Photon correlation spectroscopy (PCS) and transmission electron microscopy (TEM) were used to determine the particle-size distribution of (90)Y- and (99m)Tc-labelled antimony trisulfide (Sb(2)S(3)) and tin colloids (Sn-colloid). (90)Y-Sb(2)S(3) and (99m)Tc-Sb(2)S(3) were found to have a diameter of 28.92 +/- 0.14 and 35.61 +/- 0.11 nm, respectively, by PCS. By TEM, (90)Y-Sb(2)S(3) particles were measured to be 14.33 +/- 0.09 nm. (90)Y-labelled Sn colloid were found to exist with a d(v(max1)) of 805 nm and a d(v(max2)) of 2590 nm, by PCS, whereas (99m)Tc-Sn colloid was shown to have more than 80% of radioactive particles of approximately 910 nm by PCS. For (90)Y-labelled Sb(2)S(3) and Sn colloid, a comparison of TEM and PCS indicates that these techniques found significantly different mean diameters. TEM has an excellent resolution necessary for radiocolloid particle-sizing analysis, and it is a desirable size-measuring technique because it is more reliable than PCS

Preparation of 99mTc PLGA and its Distribution Studies

Poster presented at the 10th Conference of the Materials Research Society of Serbia - YUCOMAT 2008, Herceg Novi, Montenegro, September 8-12, 2008

Cu(II) immobilization onto a one-step synthesized poly(4-vinylpyridine-co-ethylene glycol dimethacrylate) resin: Kinetics and XPS analysis

Synthesis of an unconventional resin based on 4-vinylpyridine (4-VP) and its Cu(II) sorption behavior were studied. Three samples of macroporous crosslinked poly(4-vinylpyridine-co-ethylene glycol dimethacrylate) (P4VPE) with different porosity parameters were prepared by suspension copolymerization by varying the n-heptane amount in the inert component. The samples were characterized by mercury porosimetry, elemental analysis and X-ray photoelectron spectroscopy (XPS). The sorption of P4VPE for Cu(II) ions, determined under non-competitive conditions, was relatively rapid, i.e., the maximum capacity was reached within 30 min. The maximum experimental sorption capacity for the sample with the highest values of pore diameter and specific pore volume (sample 3, Q(eq) = 89 mg g(-1)) was 17.5 times higher than for the sample with the lowest values of pore diameter and specific pore volume (sample 1, Q(eq) = 5.1 mg g(-1)). Since the values for pyridine content in all P4VPE samples were almost the same, it was concluded that the porosity parameters have predominant influence on Cu(II) sorption rates on P4VPE. The sorption behavior and the rate-controlling mechanisms were analyzed using six kinetic models (pseudo-first order, pseudo-second order, Elovich, intraparticle diffusion, Bangham and Boyd models). XPS study clarified the nature of the formed P4VPE-Cu(II) species

An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres

Nanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography