In vitro evaluation of the modified forwarder knot used to end a continuous suture pattern in large‐gauge suture

Objective To evaluate the strength and size of forwarder end (FE) knots modified to end continuous suture lines compared with Aberdeen (AB), square (SQ), and surgeon's (SU) knots. Study design In vitro mechanical study. Study population Knotted suture. Methods Knots were tied with 2 USP (United States Pharmacopeia) polydioxanone, 2 USP, and 3 USP polyglactin 910 and tested on a universal testing machine under linear tension. Mode of failure and knot holding capacity (KHC) were recorded, and relative knot security (RKS) was calculated. Knot volume and weight were determined by digital micrometer and balance. Knot holding capacity, RKS, size, and weight between knot type, number of throws, and suture type and size were compared by using analysis of variance testing, with P  .080). Forwarder end/AB knots failed by suture breakage at the knot, whereas some SQ/SU knots unraveled. Forwarder end knots in 2 and 3 USP polyglactin 910 were 21.1% to 44.4% (1.2‐1.4 fold) smaller compared with SQ/SU knots (P < .028). Forwarder end knots in 2 and 3 USP polyglactin 910 were 40% to 99% (1.4‐2.0 fold) larger compared with AB knots (P < .001). Conclusion Forwarder end knots provided increased KHC/RKS compared with SQ/SU knots. Clinical relevance Forwarder end knots should be considered for closures when suture is placed under tension

Electrosurgical bipolar vessel sealing for a standing flank ovariectomy in beef heifers

Traditional ovariectomy techniques for heifers (e.g. colpotomy) have adverse effects ranging from hemorrhage to mortality. Electrosurgical bipolar vessel sealing devices offer a superior ligation of ovarian pedicle and associated structures. In ruminants, standing flank laparotomy has been used for abdominal visibility. We performed ovariectomy in 2 companion beef heifers via a unilateral flank approach, combined with electrosurgical ligation. Surgeries were performed in standing animals, under local anesthesia, with no observed short- or long-term adverse effects. This technique is worth considering for cases where the security of electrosurgical ligation combined with the accessibility of a flank laparotomy are desired. Additional research is needed to determine the viability of the technique for cattle of various ages and breeds

Gastrointestinal ulceration in calves presented to a central Iowa veterinary referral facility: An underappreciated morbidity?

The objective of this retrospective investigation is to identify the incidence of gastrointestinal ulceration as a co-morbidity in calves presenting to a referral veterinary hospital. Approximately 24% of calves presented to the hospital that died or were euthanized had evidence of gastrointestinal ulceration. Previous administration of an NSAID was significantly associated with the presence of ulcers, whereas antibiotic administration, age at presentation, gender, or breed were not. Clinicians and producers should consider the risk of ulceration in calves treated with NSAIDs, in light of antiulcer therapies.This is a pre-print of the article Mosichuk, Allison, Joe Smith, Dane Tatarniuk, and Amanda Kreuder. "Gastrointestinal ulceration in calves presented to a central Iowa veterinary referral facility: An underappreciated morbidity?." bioRxiv (2020): 2020-12. DOI: 10.1101/2020.12.17.423269. Copyright 2020 The Authors. Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0). Posted with permission

Meropenem Administered via Intravenous Regional Limb Perfusion for Orthopedic Sepsis in Horses: A Clinical Retrospective Study

Septic synovitis is a critical orthopedic condition in horses. Early intervention is key, with antibiotic therapy typically initiated prior to culture and susceptibility reports becoming available. The pharmacokinetics of several antibiotics have been studied in horses for use in intravenous regional limb perfusion (IVRLP) for septic synovitis, including the carbapenem antibiotic, meropenem. For a variety of factors, some veterinary clinicians may select IVRLP meropenem as therapy for these cases. Meropenem is a vital antibiotic in human medicine, making veterinary use divisive. However, verifying the efficacy of meropenem contrasted to other IVRLP antibiotics is essential for appropriate antimicrobial stewardship. To investigate this, equine patient medical records at a single veterinary teaching hospital were examined. Cases treated with meropenem or gentamicin via IVRLP for septic synovitis were retrospectively analyzed for demographics, diagnostics, treatments, outcomes, and adverse effects. Twenty-three meropenem and 37 gentamicin treated horses were analyzed; demographic information was similar between groups. In the meropenem group, nine horses received meropenem only; the remainder received another antibiotic initially then changed to meropenem. Structures infected included joints (meropenem = 13, gentamicin = 17), tendon sheaths (meropenem = 5, gentamicin = 8) and navicular bursae (meropenem = 2, gentamicin = 6). Overall survival to discharge was 86% (52/60), with meropenem 91% (21/23) and gentamicin 84% (31/37), with no statistically significant differences noted between meropenem or gentamicin groups for overall survival to discharge or outcome after discharge. Twenty-four of 26 bacterial isolates obtained from culture were reported as sensitive to imipenem, a carbapenem antibiotic similar to meropenem. Reported susceptibility to other antibiotics such as ceftiofur (n = 22/26), ampicillin (n = 18/26), amikacin (n = 15/26), or gentamicin (n = 12/26) was also frequently present. In the population of this study, antimicrobial activity augmented with IVRLP using either meropenem or gentamicin both appear to be an effective treatment for septic synovial structures, therefore, less critical antimicrobials may be a viable and more judicious treatment option.This article is published as Mosichuk, Allison P., Joseph S. Smith, Dane M. Tatarniuk, Jarrod R. Troy, and Amanda J. Kreuder. "Meropenem Administered via Intravenous Regional Limb Perfusion for Orthopedic Sepsis in Horses: A Clinical Retrospective Study." Frontiers in Veterinary Science 8 (2021): 629627. DOI: 10.3389/fvets.2021.629627. Copyright 2021 Mosichuk, Smith, Tatarniuk, Troy and Kreuder. Attribution 4.0 International (CC BY 4.0). Posted with permission

Luteoma in two mares treated by ovariectomy

A 15-year-old Paint mare (Case 1) and a 15-year-old Arabian mare (Case 2) were presented for aggressive and undesired behaviour. Reproductive evaluation revealed, initially, an enlarged ovary with a smaller/normal sized contralateral ovary in each case. Granulosa cell tumour (GCT) panel testing revealed elevated inhibin B in both cases and elevated anti-Mullerian hormone (AMH) levels only in Case 1 determined from currently established reference ranges. Based on a presumptive diagnosis of GCT, bilateral standing ovariectomy was elected for both mares. In each case neoplastic tissue consistent with luteoma was detected only on histological examination in the smaller/normal sized left ovary; the right ovary appearing normal. Long-term follow-up was performed 1 year and 5 years post-operatively by telephone. The owners were satisfied with the outcome and each patient demonstrated resolution of aggressive stallion-like behaviour. Each case returned to the owners' intended use. In conclusion, luteoma should be considered as a differential diagnosis for ovarian neoplasms in horses related to behavioural abnormalities, even in normal-sized ovaries.This is the published version of the following article: Combs, E., T. Beachler, J. R. Troy, A. Olds‐Sanchez, J. M. Howard, A. Fales‐Williams, M. Yaeger, and D. M. Tatarniuk. "Luteoma in two mares treated by ovariectomy." Equine Veterinary Education (2021). DOI: 10.1111/eve.13582. Copyright 2021 The Authors. Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Posted with permission

Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

Background: Neonatal calves are at risk of developing abomasal ulceration, but there is a lack of pharmacokinetic data for potential anti-ulcerative therapies, such as pantoprazole, in ruminant species. Objective: The study objectives were to estimate plasma pharmacokinetic parameters for pantoprazole in neonatal dairy calves after intravenous (IV) administration. A secondary objective was to quantify the concentrations of pantoprazole in edible tissues after IV dosing. Methods: Pantoprazole was administered to 9 neonatal Holstein calves at a dose of 1 mg/kg IV. Plasma samples were collected over 24 h and analyzed via HPLC-MS for determining pantoprazole concentrations. Pharmacokinetic parameters were derived via non-compartmental analysis. Tissue samples were collected at 1, 3, and 5 days after administration and analyzed via HPLC-MS. Results: Following IV administration, plasma clearance, elimination half-life, and volume of distribution of pantoprazole were estimated at 4.46 mL/kg/min, 2.81 h, and 0.301 L/kg, respectively. The global extraction ratio was estimated at 0.053 ± 0.015. No pantoprazole was detected in the edible tissues 1, 3, or 5 days after administration. A metabolite, pantoprazole sulfone was detected in all the edible tissues 1 and 3 days after administration. Conclusion: The reported plasma clearance for pantoprazole is less than that reported for alpacas but higher than reported in foals. The elimination half-life in calves appears to be longer than observed in foals and alpacas. While pantoprazole sulfone was detected in the tissues after IV administration, further research is needed as to the metabolism and potential tissue accumulation of other pantoprazole metabolites in calves. Future pharmacodynamic studies are necessary to determine the efficacy of pantoprazole on abomasal acid suppression in calves.This article is published as Olivarez, Jeff D., Amanda J. Kreuder, Dane M. Tatarniuk, Larry W. Wulf, Katarzyna A. Dembek, Jonathan P. Mochel, and Joe S. Smith. "Pharmacokinetics and tissue levels of pantoprazole in neonatal calves after intravenous administration." Frontiers in Veterinary Science 7 (2020): 580735. DOI: 10.3389/fvets.2020.580735. Copyright 2020 Olivarez, Kreuder, Tatarniuk, Wulf, Dembek, Mochel and Smith. Attribution 4.0 International (CC BY 4.0). Posted with permission

In vitro evaluation of the modified forwarder knot used to end a continuous suture pattern in large-gauge suture

Objective To evaluate the strength and size of forwarder end (FE) knots modified to end continuous suture lines compared with Aberdeen (AB), square (SQ), and surgeon's (SU) knots. Study design In vitro mechanical study. Study population Knotted suture. Methods Knots were tied with 2 USP (United States Pharmacopeia) polydioxanone, 2 USP, and 3 USP polyglactin 910 and tested on a universal testing machine under linear tension. Mode of failure and knot holding capacity (KHC) were recorded, and relative knot security (RKS) was calculated. Knot volume and weight were determined by digital micrometer and balance. Knot holding capacity, RKS, size, and weight between knot type, number of throws, and suture type and size were compared by using analysis of variance testing, with P  Results In all suture types and number of throws, FE knot KHC/RKS was 28% to 66.99% (1.2‐1.6 fold) stronger compared with SQ/SU knots (P  .080). Forwarder end/AB knots failed by suture breakage at the knot, whereas some SQ/SU knots unraveled. Forwarder end knots in 2 and 3 USP polyglactin 910 were 21.1% to 44.4% (1.2‐1.4 fold) smaller compared with SQ/SU knots (P  Conclusion Forwarder end knots provided increased KHC/RKS compared with SQ/SU knots. Clinical relevance Forwarder end knots should be considered for closures when suture is placed under tension.This article is published as Gillen, Alex, Amelia Munsterman, Erin Barrett, Stephanie Caston, Kevin Kersh, Dane Tatarniuk, Tamara Swor, and R. Reid Hanson. "In vitro evaluation of the modified forwarder knot used to end a continuous suture pattern in large-gauge suture." Veterinary Surgery (2020). DOI: 10.1111/vsu.13519. Posted with permission.</p

Electrosurgical bipolar vessel sealing for a standing flank ovariectomy in beef heifers

Traditional ovariectomy techniques for heifers (e.g. colpotomy) have adverse effects ranging from hemorrhage to mortality. Electrosurgical bipolar vessel sealing devices offer a superior ligation of ovarian pedicle and associated structures. In ruminants, standing flank laparotomy has been used for abdominal visibility. We performed ovariectomy in 2 companion beef heifers via a unilateral flank approach, combined with electrosurgical ligation. Surgeries were performed in standing animals, under local anesthesia, with no observed short- or long-term adverse effects. This technique is worth considering for cases where the security of electrosurgical ligation combined with the accessibility of a flank laparotomy are desired. Additional research is needed to determine the viability of the technique for cattle of various ages and breeds.This article is published as Smith, Joe, Guillermo Salazar, Ciara Peace, Jeff Olivarez, Katie Vermeulen, Kelley Black, Camille Guss, Dane Tatarniuk, Tyler Dohlman, and Caitlin Wiley. "Electrosurgical bipolar vessel sealing for a standing flank ovariectomy in beef heifers." Clinical Theriogenology 13, no. 1 (2021): 60-67. DOI: 10.58292/ct.v13.9364. Copyright 2021 The Authors. Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Posted with permission

Evaluation of the in vitro performance of the double forwarder knot, compared to square and surgeon's knots using large gauge suture.

ObjectiveThe aim of the study was to evaluate the strength and size of the double forwarder (DF) knot in 2 and 3 USP polyglactin 910 when used to form a ligature and to compare the knot holding capacity (KHC), size and weight of the DF knot to surgeon's (SU) and square (SQ) knots with varying numbers of throws.Study designLaboratory study.Study populationKnotted suture.MethodsKnots were tied using 2 and 3 USP polyglactin 910 and tested on a universal testing machine under linear tension. Mode of failure and (KHC) were recorded. Knot volume and weight were determined by digital micrometer and balance. KHC, size, and weight between knot type, number of throws, and suture type and size were compared using ANOVA testing, with p ResultsIn both suture types, DF knots had a higher KHC than SQ/SU knots (p  .42). All DF knots failed by suture breakage at the knot, as did all SQ/SU knots with >6 throws. DF knots in 2 and 3 USP polyglactin 910 were larger and heavier than SQ and SU knots when the same number of throws was applied (p ConclusionSelf-locking DF knots provided increased strength compared to SU/SQ in large gauge suture but only when fewer than six throws are applied to SU/SQ knots.Clinical relevanceThe new DF knot could be an alternative for a secure ligature

Case Report: Trochlear Wedge Sulcoplasty, Tibial Tuberosity Transposition, and Lateral Imbrication for Correction of a Traumatic Patellar Luxation in a Miniature Companion Pig: A Case Report and Visual Description

The objective of this case report was to describe successful surgical and post-operative management of a medial patellar luxation in a Vietnamese Potbellied Pig. A two-year old, castrated, Vietnamese Potbellied Pig presented to a veterinary teaching hospital for right pelvic limb lameness of 2 weeks duration. Upon physical examination a grade 3 patellar luxation was diagnosed on the right pelvic limb. Surgical repair included a trochlear wedge sulcoplasty, tibial tuberosity transposition, and lateral imbrication as described for canine patellar luxation. The pig was managed post-operatively with meloxicam and a physical therapy regimen of seven weeks duration. At recheck examination the pig was sound, no complications were observed, and the owners were satisfied with the outcome. As miniature companion pigs, such as Vietnamese Potbellied Pigs are currently increasing in popularity as pets, this case demonstrated that comparative techniques from other veterinary species should be considered when considering a treatment plan for a pig with a medial patellar luxation.This article is published as Høy-Petersen, Jennifer, Joe S. Smith, Paul T. Merkatoris, Kelley E. Black, Cosette M. Faivre, Kristina G. Miles, Dane M. Tatarniuk, and Karl H. Kraus. "Case report: trochlear wedge sulcoplasty, tibial tuberosity transposition, and lateral imbrication for correction of a traumatic patellar luxation in a miniature companion pig: a case report and visual description." Frontiers in Veterinary Science 7 (2021): 567886. DOI: 10.3389/fvets.2020.567886. Copyright 2021 Høy-Petersen, Smith, Merkatoris, Black, Faivre, Miles, Tatarniuk and Kraus. Attribution 4.0 International (CC BY 4.0). Posted with permission