1,236 research outputs found

    Skill Acquisition and Development Issues with Predictable Badminton Feeding Routines

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    Badminton practice designs contain feeding routines that allow players to practice technical skills. Optimal skill acquisition and development is gained through practice simulating competitive match play rather than approaches centred around mass repetition with low levels of uncertainty. This study aimed to evaluate the biomechanical differences in twelve youth elite UK badminton players in activities with varying levels of predictability. The three experimental trials were handfeed, multifeed and match play trials. Motion capture cameras were used to record participant biomechanical data. Data analysis revealed several significant biomechanical differences between trials. Results showed that an increase in trial unpredictability led to increased depth of the forward lunge shot and a lower racket shuttlecock strike position with greater forward trunk bend. This study showed that visual search behaviour in badminton is a key component of the forward lunge shot. Predictable practice feeding routines may cause poor skill acquisition and development through maladaptive learning behaviours. Coaches are recommended to implement unpredictable feeding practice routines to simulate the visual search behaviour of competitive badminton match play to enhance skill acquisition and development in youth elite badminton players

    Protective Immunity against Infection with <i>Mycoplasma haemofelis</i>

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    Hemoplasmas are potentially zoonotic mycoplasmal pathogens, which are not consistently cleared by antibiotic therapy. Mycoplasma haemofelis is the most pathogenic feline hemoplasma species. The aim of this study was to determine how cats previously infected with M. haemofelis that had recovered reacted when rechallenged with M. haemofelis and to characterize the immune response following de novo M. haemofelis infection and rechallenge. Five specific-pathogen-free (SPF)-derived naive cats (group A) and five cats that had recovered from M. haemofelis infection (group B) were inoculated subcutaneously with M. haemofelis. Blood M. haemofelis loads were measured by quantitative PCR (qPCR), antibody response to heat shock protein 70 (DnaK) by enzyme-linked immunosorbent assay (ELISA), blood lymphocyte cell subtypes by flow cytometry, and cytokine mRNA levels by quantitative reverse transcriptase PCR. Group A cats all became infected with high bacterial loads and seroconverted, while group B cats were protected from reinfection, thus providing the unique opportunity to study the immunological parameters associated with this protective immune response against M. haemofelis. First, a strong humoral response to DnaK was only observed in group A, demonstrating that an antibody response to DnaK is not important for protective immunity. Second, proinflammatory cytokine interleukin-6 (IL-6) mRNA levels appeared to increase rapidly postinoculation in group B, indicating a possible role in protective immunity. Third, an increase in IL-12p35 and -p40 mRNA and decrease in the Th2/Th1 ratio observed in group A suggest that a Th1-type response is important in primary infection. This is the first study to demonstrate protective immunity against M. haemofelis reinfection, and it provides important information for potential future hemoplasma vaccine design

    Isolating signatures of major cloud-cloud collisions - II. The lifetimes of broad bridge features

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    This article has been accepted for publication in Monthly Notices of the Royal Astronomical Society. © 2015 The Authors. Published by Oxford University Press on behalf of the Royal Astronomical Society.We investigate the longevity of broad bridge features in position–velocity diagrams that appear as a result of cloud–cloud collisions. Broad bridges will have a finite lifetime due to the action of feedback, conversion of gas into stars and the time-scale of the collision. We make a series of analytic arguments with which to estimate these lifetimes. Our simple analytic arguments suggest that for collisions between clouds larger than R ∼ 10 pc the lifetime of the broad bridge is more likely to be determined by the lifetime of the collision rather than the radiative or wind feedback disruption time-scale. However, for smaller clouds feedback becomes much more effective. This is because the radiative feedback time-scale scales with the ionizing flux Nly as R7/4N−1/4ly R7/4Nly−1/4 so a reduction in cloud size requires a relatively large decrease in ionizing photons to maintain a given time-scale. We find that our analytic arguments are consistent with new synthetic observations of numerical simulations of cloud–cloud collisions (including star formation and radiative feedback). We also argue that if the number of observable broad bridges remains ∼ constant, then the disruption time-scale must be roughly equivalent to the collision rate. If this is the case, our analytic arguments also provide collision rate estimates, which we find are readily consistent with previous theoretical models at the scales they consider (clouds larger than about 10 pc) but are much higher for smaller clouds.Peer reviewe

    Contrast Mechanisms for the Detection of Ferroelectric Domains with Scanning Force Microscopy

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    We present a full analysis of the contrast mechanisms for the detection of ferroelectric domains on all faces of bulk single crystals using scanning force microscopy exemplified on hexagonally poled lithium niobate. The domain contrast can be attributed to three different mechanisms: i) the thickness change of the sample due to an out-of-plane piezoelectric response (standard piezoresponse force microscopy), ii) the lateral displacement of the sample surface due to an in-plane piezoresponse, and iii) the electrostatic tip-sample interaction at the domain boundaries caused by surface charges on the crystallographic y- and z-faces. A careful analysis of the movement of the cantilever with respect to its orientation relative to the crystallographic axes of the sample allows a clear attribution of the observed domain contrast to the driving forces respectively.Comment: 8 pages, 8 figure

    Abatement of circumneutral mine drainage by Co-treatment with secondary municipal wastewater

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    Acid mine drainage is a persistent and problematic source of water pollution. Co-treatment with municipal wastewater at existing wastewater treatment plants has several advantages; however, potential impacts on plant physicochemical and biological processes have not been well explored. The primary purpose of this bench-scale study was to examine the impact of co-treatment by combining a mild acid mine drainage at various ratios with municipal wastewater, followed by sludge settling and supernatant comparative analysis using a variety of effluent water quality parameters. These measurements were combined with carbonate system and adsorption isotherm modeling to elucidate the mechanisms underlying the experimental results. Acid mine drainage addition decreased municipal wastewater effluent PO43− concentrations below 0.2 mg/L with greater than 97% removal, demonstrating co-treatment as an alternative solution for municipal wastewater nutrient removal. Biochemical oxygen demand remained similar to controls with \u3c10% variation after co-treatment. Coagulation from metals in acid mine drainage was incomplete due to PO43− adsorption, confirmed by comparing experimental results with Langmuir isotherm behavior. Sweep flocculation was the dominating particle aggregation mechanism, and co-treatment led to improved particle clarification outcomes. Improved clarification led to up to 50% Fe removal. Final pH had little variation with all conditions having pH \u3e 6.0. Carbonate system modeling adequately explains pH effects, and can also be applied to varying acid mine drainage matrices. The impact of acid mine drainage addition on the municipal wastewater microbial community was also investigated which provided evidence of microbial adaptation. This study demonstrates post-aeration co-treatment enables mitigation of mild acid mine drainage without adversely affecting wastewater treatment plant processes. Reported results also frame required future studies to address extant questions prior to full-scale adaptation

    Non-ribosomal phylogenetic exploration of Mollicute species:New insights into haemoplasma taxonomy

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    AbstractNine species of uncultivable haemoplasmas and several Mycoplasma species were examined by partial sequencing of two protein-encoding housekeeping genes. Partial glyceraldehyde-3-phosphate dehydrogenase (gapA) and heat shock protein 70 (dnaK) gene sequences were determined for these Mollicute species; in total nine gapA sequences and ten dnaK sequences were obtained. Phylogenetic analyses of these sequences, along with those of a broad selection of Mollicute species downloaded from GenBank, for the individual genes, and for the gapA and dnaK concatenated data set, revealed a clear separation of the haemoplasmas from other species within the Mycoplasma genus; indeed the haemoplasmas resided within a single clade which was phylogenetically detached from the pneumoniae group of Mycoplasmas. This is the first report to examine the use of gapA and dnaK, as well as a concatenated data set, for phylogenetic analysis of the haemoplasmas and other Mollicute species. These results demonstrate a distinct phylogenetic separation between the haemoplasmas and Mycoplasmas that corresponds with the biological differences observed in these species, indicating that further evaluation of the haemoplasmas’ relationship with the Mycoplasma genus is required to determine whether reclassification of the haemoplasmas is necessary

    Potential Implications of Acid Mine Drainage and Wastewater Cotreatment on Solids Handling: A Review

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    Acid mine drainage (AMD) is a persistent and extensive source of water pollution and ecological degradation. Cotreating munici- pal wastewater (MWW) with AMD using existing infrastructure at conventional wastewater treatment plants (WWTPs) may serve as a potential option for AMD abatement. However, commonly elevated iron and aluminum concentrations and low pH of AMD could negatively impact various processes at a WWTP. The focus of this mini review was on determining how cotreating MWW with AMD could impact the solids handling processes at a WWTP. While no studies have explored the solids that could be generated during cotreatment in a WWTP, numerous articles separately discuss the solids generated during AMD or MWW treatment. Reviewing this literature revealed that iron and aluminum, common metals in AMD, are already present in MWW sludge and typically benefit most solids handling processes. The addition of AMD would elevate iron and aluminum concentration but would likely result in improved sludge dewatering, removal of odor-causing compounds during processing, and a decreased bioavailability of trace metals and water-soluble P in land applications. This review concludes that cotreating MWW with moderate to low volumes (\u3c50%) of AMD at WWTPs will have minimal impact on, and likely improve, solids handling processes
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