20 research outputs found
Fractionation of oligomeric triacylglycerides and the relation to rejection limits for used frying oils
Precipitates enriched in oligomeric triacylglycerides were separated from thermally oxidized olive residue oil, conventional and high-oleic sunflower oils, and soybean oil by solvent fractionation in methanol/acetone at 4-5°C for 16 h. Different fractionation conditions were evaluated in an effort to isolate the oligomeric triacylglycerides (OTG). OTG, formed in frying oils upon heating at low concentations, were not detectable with conventional methods to determine polymeric compounds. The best conditions found from the different assays were the following: (i) weight of oil sample-to-solvent volume ratio of 1:20; and (ii) solvent system methanol/acetone 10:90 (vol/vol) for monounsaturated oils and 15:85 (vol/vol) for polyunsaturated oils. Precipitates, enriched in oligomers, were formed when heated oils and used frying oils contained more than 27% polar compounds, a value which is widely accepted as the upper limit for use of frying oils
Quantitation and distribution of altered fatty acids in frying fats
The distribution and quantity of polar compounds and altered fatty acids in used frying oils, collected by Food Inspection Services of the Junta de Andalucía in Spain, was measured. Additional samples evaluated were sunflower oil, high-oleic sunflower oil, and palm olein that were subjected to thermoxidation and frying in laboratory experiments. A combination of adsorption and high-performance size-exclusion chromatography was applied to the oil samples both before and after transesterification. Through analysis of fatty acid methyl ester derivatives, differentiation of four groups of altered fatty acids (oxidized monomers, nonpolar dimers, oxidized dimers, and polymers) could be attained. Evaluation of real frying samples with polar compound levels around the limit for fat rejection (21.1–27.6% polar compounds) gave values of total altered fatty acids ranging from 8.1 to 11.3%, and levels higher than 20% were found in the most degradated samples. The results obtained clearly support the need for control and improvement of the quality of used fats in fried-food outlets.This research work was supported by EC (Project AIR1-CT92-0687) and by CICYT (Project ALI 91-0594).Peer reviewe
Effect of standard phenolic compounds and olive oil phenolic extracts on acrylamide formation in an emulsion system.
In this work the effect of standard phenolic compounds such as trolox, phenolic acids (ferulic, gallic, protocatechuic and caffeic acid) and secoiridoids, extracted from virgin olive oil (VOO), on acrylamide formation in an emulsion system was investigated. The emulsion system was heated in an oil bath at different temperatures and for different periods of time. At 105 and 115°C the generated amounts of acrylamide were negligible during the monitoring time. At 125°C addition of standard phenolic compounds reduced acrylamide formation which was more pronounced at initial time of heating (20. min) reaching up to 70%. Protocatechuic and gallic acid were more effective than trolox, caffeic and ferulic acid in acrylamide reduction at the final time intervals of heating (30-60. min). On the contrary, addition of VOO extract in the emulsion system resulted in a remarkable increase of acrylamide level, which was ascribed to secoiridoids' structure containing aldehydic groups
Quantitation of oxidized fatty acids in frying fats and identification of major structures by GLC-MS
Trabajo presentado en el EURO FOOD CHEM VIII, celebrado en Viena (Austria) del 18 al 20 de septiembre de 1995.Used frying fats and oils from several restaurants and fried food outlets in southern Spain were collected and 6 samples of olive and/or sunflower oils were selected for analysis. Mono-, di- and polymeric groups of altered fatty acids were quantified by high performance size exclusion chromatography, and monomeric fractions were further characterized by GLC-MS. Levels of polar compounds and altered fatty acids (total, oxidized monomers, nonpolar dimers, oxidized dimers and polymers) in the oils are tabulated. Levels and distributions of altered fatty acids depended largely on alteration levels and unsaturation degrees of fats and oils, although major monomeric compounds present (in all cases, aldehydes, epoxyacids, ketoacids and hydroxyacids) appeared generally independent of differences in variables in the frying process
Characterization and classification of Western Greek olive oils according to cultivar and geographical origin based on volatile compounds
The aim of the present study was to characterize and classify olive oils from Western Greece according to
cultivar and geographical origin, based on volatile compound composition, by means of Linear Discriminant
Analysis. A total of 51 olive oil samples were collected during the harvesting period 2007–2008 from
six regions of Western Greece and from six local cultivars. Forty-five of the samples were characterized
as extra virgin olive oils. The analysis of volatile compounds was performed by Headspace Solid Phase
Microextraction-Gas Chromatography/Mass Spectrometry (HS-SPME-GC/MS). Fifty-three (53) different
volatile compounds were tentatively identified and semi-quantified. Using selected volatile compound
composition data (selection was based on the application of ANOVA to total volatiles to determine those
variables showing substantial differences among samples of different geographical origin/cultivar), the
olive oil samples were satisfactorily classified according to geographical origin (87.2%) and cultivar (74%)
Effect of storage on quality parameters and phenolic content of Italian extra-virgin olive oils
Characterisation of the geographical origin of Western Greek virgin olive oils based on instrumental and multivariate statistical analysis
In this work, measurements of free acidity, peroxide content, spectrophotometric parameters,
chlorophyll content, phenols, sterols, fatty acids and triacylglycerol composition, were carried out
on samples of virgin olive oils (VOOs) coming from four different Greek Ionian islands, i.e.
Zakynthos, Kefalonia, Lefkada and Kerkyra. An analysis of variance (ANOVA) highlighted
statistically significant differences (p<0.01) in the values of 26 analytical parameters among the
VOOs produced in the four different geographical regions but a Post-Hoc test showed that no
variable was able to distinguish all four origins. Analogously, a Principal Component Analysis
(PCA) showed a modest grouping of VOOs according to geographical origin except for Kerkyra
samples which were more distinct from others. Applying discriminant function analysis (DFA) a
good separation of the four geographical groups was achieved with classification and prediction
abilities equal to 97.7% and 95.3%, respectively. Moreover, the analysis of the standardized
coefficients showed that the fatty acids and triacylglycerols were the most discriminant variables.
This last outcome was confirmed by comparison of the prediction performances obtained applying
DFA on four subdatasets containing fatty acids (69.8%), triacylglycerols (76.7%), sterols (62.8%),
and remaining parameters (65.1%) together, respectively. As the results showed, the
multidisciplinary approach that combines different types of analytical determinations improved the
discrimination of geographical origin for Greek virgin olive oils
FTIR Spectroscopy and Multivariate Analysis Can Distinguish the Geographic Origin of Extra Virgin Olive Oils
This work investigates whether Fourier transform infrared spectroscopy (FTIR), in combination with multivariate analysis, can distinguish extra virgin olive oils from different producing countries. Duplicate spectra were collected from 60 oils from four European countries. Two approaches to data analysis were used as follows: first, the “whole spectrum” method of partial least squares (PLS) followed by distance-based linear discriminant analysis (LDA) applied to the PLS scores, and second, a genetic algorithm (GA) for variate selection from the raw data, followed by LDA applied to the selected subset. The PLS−LDA approach produced a cross-validation success rate of 96%, whereas the GA−LDA approach achieved a 100% cross-validation success rate, from subsets comprising only eight variates. Neither the selected variate nor the whole spectrum approach was able to offer insight into the origin of the discrimination in biochemical terms. However, FTIR analysis is rapid, and this work shows that it has the required discriminatory power to potentially offer a “black box” method of screening oils to verify their country of origin