Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes

We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number of sperm bound to the PVM in fresh (T1) and frozen–thawed (T2) semen from both species was compared to the sperm quality observed in routine tests. The PVM was stretched on a circular silicone device to create a standardized area for analysis. In both treatments and for both species, the spermatozoa were able to bind to the PVM, indicating that PVM may be used for a sperm binding assay in ocelot and oncilla. The S-PVM assay did not differ in fresh and frozen–thawed ocelot sperm (p > 0.05). However, fewer oncilla sperm (p < 0.05) were bound to the PVM in T2, indicating that the proposed test may be able to detect injuries that compromise sperm binding abilities. The device maintained the PVM stretched during the processing and defined the evaluation area

Monitoramento por radiotelemetria da área de uso de onça parda reintroduzida no entorno do Parque Estadual da Serra do Brigadeiro - MG, Brasil Radiotelemetry monitoring of the home range of cougar reintroduced at the surroundings of the State Park of Serra do Brigadeiro - MG, Brazil

Um exemplar macho, subadulto de onça parda reintroduzido foi monitorado por técnica de radiotelemetria. O monitoramento foi de 110 dias, realizado de forma aleatória, com intervalos descontínuos. Os dados do acompanhamento sugerem que a área utilizada por este espécime seja de, aproximadamente, 26km². Esta configura o menor índice já descrito para a espécie, porém, deve-se ressaltar a importância deste monitoramento básico, visto a incipiência do comportamento de um felino de grande porte reintroduzido em habitat natural após período em cativeiro.<br>One subadult male specimen of a reintroduced mountain lion was monitored using the radio telemetry technique. The monitoring lasted 110 days, being randomly distributed. Data of the monitoring through radiotelemetry suggested that the home range of this specimen was of approximately 26km². This sets as the smallest home range described for this species. However, the importance of this basic monitoring should be emphasized, since information of the behavior of a large feline reintroduced in its natural habitat after a period in captivity is incipient