in silico testing of primers for Scelionidae and Pentatomidae

Output for in silico testing of Scel_F1, PENT_F2, HCO-2198 on Scelionidae, Telenomus, Trissolcus, and Pentatomidae consensus sequences. Results generated using FastPCR v6.4 (Kalendar et al. 2011; http://primerdigital.com/fastpcr.html) to screen target sequences to determine primer location, orientation, efficiency of binding, and complementarity

Alignment of public sequences for the COI gene for the Scelionidae

Alignment of public sequences available on the Barcode of Life Datasystems (BOLD) for the DNA barcoding region of the COI gene for the Scelionidae. Alignment was performed in MegAlign (DNASTAR Lasergene) using ClustalW. BOLD Sequence ID number and Genus name provided for each specimen

Primer Picking Results for Scelionidae

Location, direction, suitability, and amplification parameters for primers designed to amplify Scelionidae. Primer stats generated using Primer3 v0.4.0 (Untergrasser et al. 2012; http://bioinfo.ut.ee/primer3-0.4.0/)

Primer Picking Results for Pentatomidae

Location, direction, suitability, and amplification parameters for primers designed to amplify Pentatomidae. Results generated using Primer3 v0.4.0 (Untergrasser et al. 2012; http://bioinfo.ut.ee/primer3-0.4.0/)

Alignment of COI barcode sequences for Telenomus spp. (Hymenoptera: Scelionidae)

Alignment of sequences for the DNA barcoding region of the COI gene for species of Telenomus collected in the field in Canada, Switzerland, and China. Alignment was performed in MegAlign (DNASTAR Lasergene) using ClustalW. BOLD Sequence ID number and Genus name provided for each specimen

Alignment of public sequences for the COI gene for the Pentatomidae

Alignment of public sequences available on the Barcode of Life Datasystems (BOLD) for the DNA barcoding region of the COI gene for the Pentatomidae. Alignment was performed in MegAlign (DNASTAR Lasergene) using ClustalW. BOLD Sequence ID number and Genus name provided for each specimen