Individual Professional Practice in the Company

Import 23/08/2017Cílem této bakalářské práce je popsat absolvování odborné praxe ve firmě HS Interactive s.r.o. Praxe byla zaměřena na vývoj mobilní aplikace pro operační systém Android. Aplikace je mobilním klientem pro sociální síť MatchToMe. V úvodu popisuji důvody, které vedly k výběru odborné praxe. Dále se věnuji úkolům, které mi byly zadány s jejich implementací a postupem řešení problémů, které se objevily při vývoji. Závěr práce je věnován zhodnocení získaných zkušeností a dosažených výsledků.Purpose of this bachelor thesis is to describe a professional practice in company HS Interactive s.r.o. Practice was focused on the development of mobile application for the operating system Android. The application is a mobile client for social network MatchToMe. In the introduction I describe reasons that led to the selection of professional practice. Then I describe tasks that I have been awarded with their implementations and process of solution issues that have emerged during development. The conclusion of thesis is dedicated to the evaluation of the experience gained and the results achieved.440 - Katedra telekomunikační technikyvýborn

Urban expansion patterns of 291 Chinese cities, 1990–2015

<p>China has experienced rapid and massive urban expansion in recent decades. To address the opportunities and challenges posed by urbanization, it is important to investigate this process. Although many studies have characterized China’s urbanization, mainly based on individual cities or urban agglomerations, few studies have compared the urban expansion patterns among cities of different regions and sizes at a national scale. Additionally, the source of the newly developed urban land has largely been ignored in previous research. To fill these gaps, we provide a comprehensive view of the growth patterns of 291 Chinese cities during 1990–2015, quantifying the rates, spatial forms, and corresponding landscape characteristics of the urban expansion. More importantly, we further investigate what their new urban land is converted from. Key findings include: (1) the annual expansion (AE) and annual growth rate (AGR) of all 291 cities were 1869.81 km² and 4.81% during 1990–2015; (2) cropland and rural settlements were the predominant source, accounting for 65.22% and 17.49% of the new urban land during 1990–2015; (3) edge-expansion was the dominant growth form for most cities, followed by infilling and leapfrogging growth; and (4) the landscape characteristics of the occupied cropland differed across various cities.</p

The effects of immune protein CD3ζ development and degeneration of retinal neurons after optic nerve injury

<div><p>Major histocompatibility complex (MHC) class I molecules and their receptors play fundamental roles in neuronal death during diseases. T-cell receptors (TCR) function as MHCI receptor on T-cells and both MHCI and a key component of TCR, CD3ζ, are expressed by mouse retinal ganglion cells (RGCs) and displaced amacrine cells. Mutation of these molecules compromises the development of RGCs. We investigated whether CD3ζ regulates the development and degeneration of amacrine cells after RGC death. Surprisingly, mutation of CD3ζ not only impairs the proper development of amacrine cells expressing CD3ζ but also those not expressing CD3ζ. In contrast to effects of MHCI and its receptor, PirB, on other neurons, mutation of CD3ζ has no effect on RGC death and starburst amacrine cells degeneration after optic nerve crush. Thus, unlike MHCI and PirB, CD3ζ regulates the development of RGCs and amacrine cells but not their degeneration after optic nerve crush.</p></div

The MLEs of the QTL position and effect parameters based on 200 simulation replicates under different heritabilities and sample sizes.

<p>The squared roots of the mean squared errors (RMSE) of the MLEs are given in parentheses.</p><p>The locations of the QTL is described by the map distances (in cM) from the first marker of the linkage group. The hypothesized value is 3.81 for , 2.04 for and 1.26 for .</p

RGC death after ONC is associated with quick dendritic reorganization of SACs/DSACs.

<p>(<b>A</b>) Optic nerve exposing and crushing. (<b>B</b>) An image of the longitude cross section of the proximal portion of the optic nerve and the posterior portion of the eye. RGCs and their axons are labeled with CTB conjugated with Alexa Fluor 594 (yellow) and the section was co-labeled with DAPI (red). Please note that the axonal transport of CTB along RGC axons is completely blocked at the crush site. (<b>C</b>) Magnification from whole mount retina of a mouse without ONC showing the density of DAPI stained nuclei in the GCL (C1, blue), the density of anti-ChAT stained DSACs (C2, red) and the overlay of the DAPI and anti-ChAT stainings (C3). (<b>D</b>) Magnification from whole mount retina of a mouse with ONC showing the density of DAPI stained nuclei in the GCL (D1, blue), the density of anti-ChAT stained DSACs (D2, red) and the overlay of the DAPI and anti-ChAT stainings (D3). (<b>E</b>) Representative maximum projection images of DSACs without (E1) and with (E2) ONC. (<b>F</b>) The tracing results of the DSACs shown in panel E.</p

Quantify dendritic structure of mouse SACs/DSACs.

<p>(<b>A</b>) Whole mount retina of a CreER-ChAT:Stop-YFP mouse stained with anti-GFP (green) and DAPI (blue). The four dashed-line boxes indicate the areas used for cell density calculation. (<b>B</b>) An enlarged area of the retinal ganglion cell layer showing YFP staining of Cre activated DSACs (green), anti-ChAT antibody staining of all DSACs (red) and DAPI (blue) staining of the GCL. (<b>C</b>) A cross section of the retina of a CreER-ChAT:Stop-YFP mouse showing YFP staining of Cre activated SACs and DSACs (green) and anti-ChAT antibody staining of SACs and DSACs (red) in the retina. (<b>D</b>) A maximum projection of the dendrites of a DSAC. (<b>E</b>) The tracing results of the DSAC shown in panel D (green, dendrites; red, soma; blue, dendritic field). (<b>F</b>) The dendrogram of the DSAC shown in panel D. The total length of dendrites, the number of dendritic branches, the order of each dendritic branch and the length of each dendritic branch were derived from this dendrogram. (<b>G</b>) Average size of dendritic field of SACs and DSACs. (<b>H</b>) Average length of dendrites of SACs and DSACs. (<b>I</b>) Average density of SACs and DSACs. (<b>J</b>) The number of dendritic branch as a function of dendritic order of SACs and DSACs. (<b>K</b>) The average number of dendritic branch of SACs and DSACs. (<b>L</b>) The length of dendritic branch as a function of dendritic order of SACs and DSACs. (<b>M</b>) The average length of dendritic branch of SACs and DSACs. The numbers in the columns of panels G, H, K and M indicate number of cells analyzed. The numbers in the columns of panel I indicate the numbers of images analyzed. In this figure and all following figures, * indicates 0.01</p

QTL genotypes and corresponding genetic components under different backcross designs.

<p>QTL genotypes and corresponding genetic components under different backcross designs.</p

Mutation of CD3ζ impairs the development of SACs/DSACs.

<p>(<b>A</b>) Representative maximum projection images with the dendritic tracing of SACs and DSACs of WT and CD3ζ-/- mice. (<b>B</b>) A cross section of the retina of a WT mouse showing the co-labeling of ChAT (red) and CD3ζ (green) antibody staining (scale bar: 20μm). (<b>C</b>) A cross section of the retina a CD3ζ-/- mouse showing anti-ChAT antibody staining of SACs/DSACs (red) and DAPI (blue) staining of the retina (scale bar: 20μm). (<b>D</b>) Average size of the dendritic field of SACs and DSACs in both WT and CD3ζ-/- mice. (<b>E</b>) The total dendritic length of SACs and DSACs in both WT and CD3ζ-/- mice. (<b>F</b>) The average densities of DAPI stained nuclei of GCL in both WT and CD3ζ-/- mice. (<b>G</b>) The average densities of SACs in both WT and CD3ζ-/- mice. (<b>H</b>) The average densities of DSACs in both WT and CD3ζ-/- mice. The numbers in the columns of panels D-E indicate the number of cells analyzed. The numbers in the columns of panels F-H indicate the numbers of images analyzed.</p

Quantitative analysis of the dendritic structure of SACs/DSACs after ONC.

<p>(<b>A</b>) The average densities of DAPI stained nuclei of GCL of mice with and without ONC. (<b>B</b>) The average densities of anti-ChAT antibody stained SACs and DSACs before and after ONC. (<b>C</b>) Average total length of dendrites of SACs and DSACs of mice before and after ONC. (<b>D</b>) Average size of dendritic field of SACs and DSACs of mice before and after ONC. (<b>E</b>) The number of dendritic branch as a function of dendritic order of SACs before and 10 days after ONC. (<b>F</b>) The number of dendritic branch as a function of dendritic order of DSACs before and 10 days after ONC. (<b>G</b>) The length of dendritic branch as a function of dendritic order of SACs before and 10 days after ONC. (<b>H</b>) The length of dendritic branch as a function of dendritic order of DSACs before and 10 days after ONC. The numbers in the columns of panels A-B indicate number of images analyzed. The numbers in the columns of panels C-D indicate the numbers of cells analyzed.</p

Mutation of CD3ζ does not alter the dendritic reorganization of SACs/DSACs after ONC.

<p>(<b>A</b>) Magnification from whole mount retina of a CD3ζ-/- mouse without ONC showing the density of DAPI stained nuclei in the GCL (A1), the density of anti-ChAT stained DSACs (A2) and the overlay of the DAPI and anti-ChAT stainings (A3). (<b>B</b>) Magnification from whole mount retina of a CD3ζ-/- mouse with ONC showing the density of DAPI stained nuclei in the GCL (B1), the density of anti-ChAT stained DSACs (B2) and the overlay of the DAPI and anti-ChAT stainings (B3). (<b>C</b>) The densities of DAPI stained nuclei of GCL of WT and CD3ζ-/- mice before and 10 days after ONC. (<b>D</b>) The densities of SACs of WT and CD3ζ-/- mice before and 10 days after ONC. (<b>E</b>) The densities of DSACs of WT and CD3ζ-/- mice before and 10 days after ONC. (<b>F</b>) The size of dendritic field of SACs of WT and CD3ζ-/- mice before and 10 days after ONC. (<b>G</b>) The size of dendritic field of DSACs of WT and CD3ζ-/- mice before and 10 days after ONC. (<b>H</b>) The total length of dendrites of SACs of WT and CD3ζ-/- mice before and 10 days after ONC. (<b>I</b>) The total length of dendrites of DSACs of WT and CD3ζ-/- mice before and 10 days after ONC. The numbers in the columns of panels B-D indicate number of images analyzed. The numbers in the columns of panels E-H indicate the numbers of cells analyzed.</p