6 research outputs found
Type 2 diabetes mellitus and dyslipidaemia: effects of genetic variation in African populations
Background: Low-density lipoproteins (LDL) have been associated with damage to the
cardiovascular system in patients with type 2 diabetes mellitus (T2DM). These patients are
two (2X) to four (4X) times more likely to develop cardiovascular diseases (CVD) compared
to non-diabetic patients due to dysfunctional lipoprotein metabolism. Normal lipid
metabolism involves interconversion and transfer of molecules regulated by several enzymes
such as Apolipoprotein E (ApoE) and proprotein convertase subtilisin/kexin type 9 (PCSK9).
ApoE and PCSK9 are involved in clearance of lipoproteins and therefore, influence lipid
profiles. Association between ApoE and T2DM in cardiovascular diseases have been widely
reported. PCSK9 on the other hand is emerging as an important player in lipid metabolism
but its effects in diabetes are not known. Studies on both ApoE and PCSK9 in African
populations are in its infancy. Each year, CVD kills more people than any other cause of
death. Many CVDs can be traced back to pathological process of atherosclerosis, in which
fatty material collects along walls of arteries, limiting flexibility and obstructing blood flow.
T2DM alone has been classified as a major factor for development of CVD and one of its
complications is the development of dyslipidaemia. Unlike PCSK9, genetic polymorphisms
in ApoE have been well characterised as important dyslipidaemia genetic markers associated
with coronary artery disease. The association between ApoE and PCSK9 gene
polymorphisms with dyslipidaemia in T2DM was evaluated. Diabetic dyslipidaemia presents
as a triad of high triglycerides, high LDL and low high-density lipoprotein (HDL).
Aims and Objectives: This study aimed to evaluate the role of genetic variation in genes
coding for ApoE2 and PCSK9 on dyslipidaemia in South African diabetic patients. Main
objectives’ included recruitment of participants, genetic characterisation of ApoE and PCSK9
and determination of the lipid profiles for the recruited participants.
Methods: Two hundred and forty-four (n=244) participants were recruited from the
Baragwaneth diabetic clinic, using a retrospective approach. The participants comprised of
two groups, (i) dyslipidaemic, and (ii) non-dyslipidaemic (controls). The dyslipidaemic group
was further divided into three groups; i) those with high cholesterol only, ii) those with high
triglycerides only and iii) those with both high cholesterol and triglycerides which is referred
to as the mixed group. Clinical and demographic parameters were retrieved from hospital
records with the consent of the participants. Ethical clearance was obtained from the University of Cape Town and University of Witwatersrand. Genetic characterisation of ApoE
was carried out using polymerase chain reaction (PCR) coupled to restriction fragment length
polymorphism (RFLP) and confirmed through sequencing while characterisation for PCSK9
was carried out through Sanger sequencing.
Results: Of the 244 participants, 165 were dyslipidaemic while 79 were not dyslipidaemic.
The 165 dyslipidaemic participants were further divided into 33.3% (n=55) those with high
cholesterol, 29.1% (n=48) those with high triglycerides and 37.6% and (n= 62) those with
high cholesterol and triglycerides (mixed). The cohort comprised of 128 (52%) females,
median (IQR) age 56.0 (48.0 – 64.0) years and 116 (48%) males with median (IQR) age of
56.5 (48.0 – 63.0) years. Most of the characteristics between the dyslipidaemic and nondyslipidaemic participants were significantly different as expected in a purposive sampling
technique. ApoE3/4 genotype had the highest frequency distribution (41%) while ApoE2/3
genotype had the lowest frequency (7%). An uncharacterised ApoE referred to in the study as
ApoE X with a frequency distribution of 6%, was reported for the first time. The selected
measured parameters evaluated against a set of variables showed a significant association
between HbA1c and age (p=<0.008) is reported. TC (p=0.00092), LDL (p=0.0184) and TG
(p=0.0175) were strongly associated with poor glycaemic. Both LDL (p=0.0174 and HDL
(p=0.0072) were associated with age. Homozygous ApoE2/2 and heterozygous ApoE2/3
genotypes correlated with poor glycaemic control with a median HbA1c of 10.95% (IQR
5.88-14.98%) and 10.20% (IQR 6.20-15.80%), respectively; while homozygous ApoE4/4
carriers displayed good glycaemic control with a median HbA1c of 6.60% (IQR 5.70 –
2.30%). Carriers of homozygous ApoE3/3 genotype had the highest median TC of
6.06mmol/L (IQR 5.48 -– 6.71mmol/L) while homozygous ApoE4/4 carriers had the highest
median triglycerides of (2.94 (IQR 1.75 – 5.13 mmol/L). Carriers of homozygous PCSK9
rs505151 A/A (E670G) genotype had the highest frequency distribution in both groups of
participants with dyslipidaemic (55.1%) and non-dyslipidaemic (63. 5%), followed by
carriers of heterozygous PCSK9 rs505151G/A at 40.6% and lastly carriers of PCSK9
rs505151G/G at (9.5%). On the other hand, carriers of homozygous PCSK9 rs28362286 C/C
genotype were predominantly distributed with a frequency of 94.2% and
PCSK9rs28362286C/A had a very small frequency distribution of 5.8% while
PCSK9rs28362286A/A was absent in this population. Carriers of PCSK9 rs505151A/A
genotype had higher HbA1c with a median of 10.10% (IQR 7.48 – 12.90) compared to PCSK9 rs505151 G/A genotype with a median of 9.00% (IQR 7.03 –11.35). The results
show that PCSK9 rs505151G/A with lower HbA1c had non-significantly higher TC, LDL,
TG and non-HDL but lower HDL compared to PCSK9 rs505151A/A genotype. The results
revealed no direct reciprocal relationship between glycaemic control and level or type of
dyslipidaemia.
Conclusions: The study showed the effects of ApoE and PCSK9 genetic variation on the
dyslipidaemia seen in black South African diabetic participants. Therefore, this study through
ApoE and PCSK9 genotypes show that the diabetic dyslipidaemia has an underlying genetic
influence. In addition, to the well-characterised ApoE genotypes, an uncharacterised
genotype referred to as ApoE X genotype is reported. With these findings, consideration to
explore possible underlying genetic predisposition is recommended especially in diabetic
patients with dyslipidaemia that responds poorly to standard therapy
The influence of ApoE genotype on the lipid profile and lipoproteins during normal pregnancy in a Southern African population
Background: Pregnancy is associated with increases in fasting triglycerides and total cholesterol.1 ApoE isoforms are known to influence the concentration of cholesterol, with apoE2 homozygosity lowering and apoE4 homozygosity raising the cholesterol concentration compared with E3 homozygosity.2 The lipid profiles ApoE status and prevalence of small dense LDL species were evaluated for subjects attending an antenatal clinic.Results: Samples from 690 women aged between 16 and 42 years of age were analyzed during and after pregnancy. The fasting plasma triglyceride concentration (in mmol/L) was significantly higher in pregnancy (median = 1.5, IQR 1.0-2.0 vs median = 0.6, IQR 0.5-0.8 respectively, p < 0.0001). Similarly, the total cholesterol (in mmol/L) was increased during pregnancy (median=4.1, IQR 3.6-4.7 vs median 3.5, IQR 3.1-3.5, respectively p=0.0167). The median LDL cholesterol and HDL cholesterol did not change. Higher proportions of small density LDL species were seen during pregnancy compared to after pregnancy. The distribution of the LDL species during pregnancy and 6 weeks post-partum were significantly different p<0.0001 with the smaller species being much higher during pregnancy.Conclusion: ApoE4 genotype was associated with increased total cholesterol and LDL cholesterol concentrations during pregnancy. Pregnancy results in a reversible remodeling of LDL to smaller species, the significance of which is unknown but mayindicate a predisposition to atherosclerosisKeywords: Apolipoprotein E. Lipids, pregnancy, small dense LDL species, African.Due to errors in the previous PDF especially in the 'Cite as' authors names, the PDF fulltext has been reloaded. This information is now correct
The influence of ApoE genotype on the lipid profile and lipoproteins during normal pregnancy in a Southern African population.
Background: Pregnancy is associated with increases in fasting
triglycerides and total cholesterol. ApoE isoforms are known to
influence the concentration of cholesterol, with apoE2 homozygosity
lowering and apoE4 homozygosity raising the cholesterol concentration
compared with E3 homozygosity. The lipid profiles ApoE status and
prevalence of small dense LDL species were evaluated for subjects
attending an antenatal clinic. Results: Samples from 690 women aged
between 16 and 42 years of age were analyzed during and after
pregnancy. The fasting plasma triglyceride concentration (in mmol/L)
was significantly higher in pregnancy (median = 1.5, IQR 1.0-2.0 vs
median = 0.6, IQR 0.5-0.8 respectively, p < 0.0001). Similarly, the
total cholesterol (in mmol/L) was increased during pregnancy
(median=4.1, IQR 3.6-4.7 vs median 3.5, IQR 3.1-3.5, respectively
p=0.0167). The median LDL cholesterol and HDL cholesterol did not
change. Higher proportions of small density LDL species were seen
during pregnancy compared to after pregnancy. The distribution of the
LDL species during pregnancy and 6 weeks post-partum were significantly
different p<0.0001 with the smaller species being much higher during
pregnancy. Conclusion: ApoE4 genotype was associated with increased
total cholesterol and LDL cholesterol concentrations during pregnancy.
Pregnancy results in a reversible remodeling of LDL to smaller species,
the significance of which is unknown but may indicate a predisposition
to atherosclerosi
Apolipoprotein E variants, plasma lipids, lipoproteins and dysApolipoprotein E variants, plasma lipids, lipoproteins and dysβLipoproteinaemia during pregnancy in Zimbabwean women.
Includes bibliographical references (leaves 117-138).This study of pregnant women in Zimbabwe therefore set itself the following aims: To describe lipid and lipoproteins during and after pregnancy, To examine the prevalence of apoE variants, To evaluate dysβlipoproteinaemia in pregnancy, The correlation between dysβplipoproteinaemia and the apoE genotypes. This is the first study to systematically examine lipids and lipoproteins during pregnancy in black Africans