第925回千葉医学会例会・第20回千葉大学医学部放射線医学教室同門会例会

Teriflunomide does not affect cell death in cultured primary microglia. Isolated microglia were treated with LPS (100 ng/ml) and different concentrations of teriflunomide (0.25–5 μM) for 12, 48, or 72 h followed by staining with propidium iodide (PI). (A, B, C) The percentage of gated PI+ CD11b/c+ cells is presented as mean + SD (n = 4). (TIF 718 kb

Additional file 3: Figure S1. of In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia

Teriflunomide inhibits rodent T cell proliferation. Single-cell suspensions from spleens of adult Sprague-Dawley rats (Crl:CD) were prepared in complete IMDM medium. Freshly isolated rat CD4+ T cells were labeled with 2.5 μM CFSE, stimulated with plate-bound anti-CD3/CD28 mAb and treated with different concentrations of teriflunomide (0.25–5 μM) for 65–72 h. Control presents unstimulated CFSE-labeled cells. (A) Total number of T cells as shown in histograms for 0.5 μM teriflunomide was determined by flow cytometry. (B) For comparison of proliferation, the number of proliferating T cells (n = 4) repeated measures ANOVA with Bonferroni’s multiple comparison t test were used. Significant effects are indicated by asterisks (***p < 0.001). (TIF 162 kb