Additional file 3: Figure S1. of In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
Teriflunomide inhibits rodent T cell proliferation. Single-cell suspensions from spleens of adult Sprague-Dawley rats (Crl:CD) were prepared in complete IMDM medium. Freshly isolated rat CD4+ T cells were labeled with 2.5 μM CFSE, stimulated with plate-bound anti-CD3/CD28 mAb and treated with different concentrations of teriflunomide (0.25–5 μM) for 65–72 h. Control presents unstimulated CFSE-labeled cells. (A) Total number of T cells as shown in histograms for 0.5 μM teriflunomide was determined by flow cytometry. (B) For comparison of proliferation, the number of proliferating T cells (n = 4) repeated measures ANOVA with Bonferroni’s multiple comparison t test were used. Significant effects are indicated by asterisks (***p < 0.001). (TIF 162 kb
